【摘要】：本文研究了基于CdSe量子點(diǎn)和Au納米粒子的毛細(xì)管電泳電化學(xué)分析研究。首先研究了CdSe量子點(diǎn)和CdSe/ZnS核殼結(jié)構(gòu)量子點(diǎn)的合成方法,成功合成且進(jìn)行了一系列表征以及性能比較;將合成的CdSe量子點(diǎn)和CdSe/ZnS核殼結(jié)構(gòu)量子點(diǎn)與毛細(xì)管電泳進(jìn)行聯(lián)用,構(gòu)建出毛細(xì)管電泳電化學(xué)發(fā)光平臺(tái),檢測(cè)了不同種類生物胺分子對(duì)量子點(diǎn)的增強(qiáng)作用,并同時(shí)對(duì)其進(jìn)行分離檢測(cè);同時(shí)分析分離檢測(cè)了萊克多巴胺和克侖特羅,并對(duì)實(shí)際樣品進(jìn)行檢測(cè),得到了較好的效果。本文還探討了基于納米金輔助信號(hào)生成順序堆積的毛細(xì)管電泳免疫分析研究。實(shí)驗(yàn)中利用納米金(AuNPs)作為載體,將辣根過(guò)氧化物酶(HRP)和毒素抗體(Ab)同時(shí)固定到納米金(Au NPs)表面,該方法通過(guò)納米金輔助信號(hào)生成和順序堆積在線富集技術(shù)實(shí)現(xiàn)了對(duì)扇貝樣品中BTX-B的快速靈敏檢測(cè)。全文共分為五章:第一章系統(tǒng)介紹了毛細(xì)管電泳(CE)的發(fā)展歷程和應(yīng)用前景,以及它的基本理論、進(jìn)樣方法、富集技術(shù)等;介紹了電化學(xué)發(fā)光(ECL)的基本原理、電化學(xué)發(fā)光體系、反應(yīng)類型、特點(diǎn)等;詳細(xì)介紹了量子點(diǎn)特點(diǎn)、發(fā)光原理、電化學(xué)發(fā)光方式以及合成方法和應(yīng)用等等;簡(jiǎn)要介紹了納米粒子的有關(guān)概念及應(yīng)用,包括金納米粒子的特點(diǎn)及應(yīng)用。第二章探討了CdSe量子點(diǎn)和CdSe/ZnS核殼結(jié)構(gòu)量子點(diǎn)的合成方法,并對(duì)實(shí)驗(yàn)中合成的兩種量子點(diǎn)進(jìn)行表征以及性能分析。第三章主要運(yùn)用合成的CdSe量子點(diǎn)與毛細(xì)管電泳聯(lián)用,構(gòu)建出一種毛細(xì)管電泳量子點(diǎn)電化學(xué)發(fā)光分析平臺(tái),并對(duì)胺類物質(zhì)進(jìn)行檢測(cè)。我們對(duì)實(shí)驗(yàn)條件進(jìn)行優(yōu)化,具體包括了實(shí)驗(yàn)所用緩沖溶液的種類,pH,濃度,以及分離電壓、檢測(cè)電位,進(jìn)樣時(shí)間等。并且在最優(yōu)條件下,利用構(gòu)建的電化學(xué)分析平臺(tái)進(jìn)行胺類物質(zhì)混合樣品的檢測(cè)。第四章運(yùn)用合成的CdSe/ZnS核殼結(jié)構(gòu)量子點(diǎn)與毛細(xì)管電泳相結(jié)合,建成一種毛細(xì)管電泳量子點(diǎn)電化學(xué)發(fā)光平臺(tái)。我們繼續(xù)探索了實(shí)驗(yàn)影響因素,包括實(shí)驗(yàn)所用緩沖溶液的種類,pH,濃度,以及分離電壓、檢測(cè)電位,進(jìn)樣時(shí)間等,并在最優(yōu)條件下,對(duì)萊克多巴胺和克倫特羅進(jìn)行分析分離檢測(cè)。第五章介紹了一種海生毒素短裸甲藻毒素(BTX)的污染現(xiàn)狀及傳統(tǒng)檢測(cè)方法,并且探索了一種納米材料與毛細(xì)管電泳相結(jié)合的新型檢測(cè)方式;進(jìn)行了一系列實(shí)驗(yàn)條件的優(yōu)化,找出最優(yōu)條件;在最優(yōu)條件下測(cè)定了貝類樣品中的短裸甲藻毒素(BTX)。
[Abstract]:The electrochemical analysis of capillary electrophoresis based on CdSe quantum dots and Au nanoparticles was studied in this paper. Firstly, the synthesis method of CdSe quantum dots and CdSe/ZnS core-shell quantum dots was studied, and a series of characterization and performance comparison were carried out successfully. The synthesized CdSe quantum dots and CdSe/ZnS core-shell structure quantum dots were combined with capillary electrophoresis. A capillary electrophoretic electroluminescent platform was constructed to detect the enhancement of quantum dots by different kinds of biogenic amine molecules and the separation and detection of ractopamine and clenbuterol. And the actual samples were detected and good results were obtained. The immunoassay of capillary electrophoresis based on the stacking of nanocrystalline gold-assisted signals was also discussed in this paper. In the experiment, (AuNPs) was used as carrier to immobilize horseradish peroxidase (HRP) and toxin antibody (Ab) to the surface of nano-gold (Au NPs) at the same time. The rapid and sensitive detection of BTX-B in scallop samples was realized by means of nanocrystalline gold assisted signal generation and sequential stacking on-line enrichment. The thesis is divided into five chapters: the first chapter systematically introduces the development course and application prospect of capillary electrophoresis (CE), its basic theory, sample injection method, enrichment technology, and introduces the basic principle of electroluminescent (ECL), electroluminescent system, and so on. The characteristics of quantum dots, luminescence principle, electrochemiluminescence methods, synthesis methods and applications are introduced in detail. The concepts and applications of nanoparticles are briefly introduced, including the characteristics and applications of gold nanoparticles. In chapter 2, the synthesis methods of CdSe quantum dots and CdSe/ZnS core-shell quantum dots are discussed. In chapter 3, a platform for electrochemiluminescence analysis of quantum dots in capillary electrophoresis was constructed by combining the synthesized CdSe quantum dots with capillary electrophoresis, and the amines were detected. We optimized the experimental conditions, including the pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. And under the optimal conditions, the electrochemical analysis platform was used to detect the mixed amines. In chapter 4, we combine the synthesized CdSe/ZnS core-shell quantum dots with capillary electrophoresis to construct a kind of electrochemiluminescence platform for capillary electrophoresis quantum dots. We continue to explore the experimental factors, including pH, concentration, separation voltage, detection potential and injection time of the buffer solution used in the experiment. Under the optimal conditions, the ractopamine and clenbuterol were analyzed and detected. In chapter 5, the pollution status and traditional detection methods of marine toxin (BTX) are introduced, and a new detection method combining nano-material with capillary electrophoresis is explored, and a series of experimental conditions are optimized. The optimum conditions were found and the (BTX). In shellfish samples was determined under the optimum conditions.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB383.1;O657.1

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