發(fā)布時(shí)間：2018-09-06 11:58

【摘要】：以聚乙二醇-聚乳酸-聚乙醇酸嵌段共聚物(PEG-PLGA)為囊材,添加脫鎂葉綠酸作為光敏劑,采用復(fù)乳法制備了光響應(yīng)的眼鏡蛇神經(jīng)毒素納米囊。以納米囊的包封率、載藥量和粒徑為指標(biāo),采用單因素法對納米囊的制備條件進(jìn)行優(yōu)化;以差示量熱掃描分析其熱流變性能,并以累積釋藥量研究其光控釋行為。優(yōu)化的PEGPLGA、眼鏡蛇神經(jīng)毒素及光敏劑脫鎂葉綠酸質(zhì)量配比為40∶12.5∶1,獲得的納米囊包封率為72.3%±3.6%,載藥量為15.1%±1.3%,平均粒徑為(862±23)nm,電位為(-46.5±3.8)m V,呈緊密球形,光敏劑分布在囊殼;在650 nm半導(dǎo)體激光照射30 min,體外釋放明顯加快。該納米囊在不光照時(shí)具有增強(qiáng)藥物穩(wěn)定和緩釋作用,而紅光可促進(jìn)藥物釋放,因而可實(shí)現(xiàn)光控靶向。
[Abstract]:The optically responsive cobra neurotoxin nanocapsules were prepared using poly (ethylene glycol) polylactic acid-polyethanolid block copolymer (PEG-PLGA) as capsule material and demagnesium chloric acid as Guang Min. Based on the encapsulation efficiency, drug loading and particle size, the preparation conditions were optimized by single factor method, and the thermal fluidity was analyzed by differential scanning calorimetry (DSC), and the photoluminescence behavior was studied by cumulative drug release. The optimized mass ratio of PEGPLGA, neurotoxin and Guang Min was 40: 12.5: 1, the encapsulation efficiency was 72.3% 鹵3.6, the drug loading was 15.1% 鹵1.3 and the mean particle size was (862 鹵23) nm, potential (-46.5 鹵3.8) m V,) was compact spherical, and Guang Min was distributed in the capsule. In vitro release was accelerated at 650 nm semiconductor laser irradiation for 30 min,. The nanoparticles can enhance drug stability and sustained release without illumination, while red light can promote drug release, so it can achieve light-controlled targeting.
【作者單位】： 廣東嶺南職業(yè)技術(shù)學(xué)院;華南理工大學(xué)化學(xué)與化工學(xué)院;
【基金】：國家自然科學(xué)基金(81173646) 廣州市科技計(jì)劃項(xiàng)目(1563000123)~~
【分類號】：TB383.1;TQ460.1

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