本文選題：納米容器 + 介孔二氧化硅納米微球(MSNs)�。� 參考：《南京理工大學(xué)》2016年碩士論文

【摘要】：納米容器通常是指具有納米尺寸并能夠存儲、釋放目標(biāo)物質(zhì)的復(fù)合材料。由于介孔二氧化硅納米微球(MSNs)具有包裹量大、表面易改性及生物無毒性等優(yōu)點,已作為理想的納米容器應(yīng)用在生物醫(yī)藥領(lǐng)域中。特別是具有刺激響應(yīng)性能的介孔二氧化硅納米容器,通過外界刺激,納米容器能夠作出刺激響應(yīng),實現(xiàn)對客體分子的裝載與釋放。常見的外界刺激有pH、溫度和氧化還原反應(yīng)等,其中以pH作為外界刺激的研究最多。為此,本文以構(gòu)建酸性刺激響應(yīng)智能納米容器藥物載體為目標(biāo),開展了酸性刺激響應(yīng)介孔二氧化硅納米容器的制備及其藥物分子的酸性可控釋放研究,具體的工作如下。以S tober方法制得的介孔二氧化硅納米微球(MSNs)作為納米容器,吸附藥物分子羅丹明B,首次使用新物質(zhì)2-(2-溴-乙氧基)-2-(2-疊氮基-乙氧基)丙烷將氨基-p-環(huán)糊精連接到納米容器的介孔處封堵羅丹明B,形成一種新型具有靶向抗癌作用的酸性智能納米容器。采用掃描透射電子顯微鏡(SEM/TEM), X射線衍射(XRD)和比表面積分析(BET)等手段表征MSNs形貌和結(jié)構(gòu)；使用固體核磁共振(SS NMR)、傅里葉紅外光譜(FTIR)和熱重分析(TGA)等手段驗證了MSNs表面改性過程。利用熒光分光光度計(RF-5201)實時監(jiān)測該智能納米容器在不同酸性pH值下釋放的動力學(xué)曲線；通過紫外分光光度計(UV-Vis)測得該智能納米容器的載藥量為15.23 mg/g。實驗結(jié)果表明,利用末尾封端技術(shù)制得的以縮酮鏈作為刺激響應(yīng)單元的智能納米容器實現(xiàn)了在中性條件下“零釋放”,酸性條件下大量釋放(仿抗癌靶標(biāo)溶酶體和內(nèi)涵體條件下pH=4-5適量緩慢釋放)的效果,此項研究在腫瘤細胞靶向方面具有很好的應(yīng)用前景。同時又由于其具有在中性條件下“零釋放”、酸性條件下大量釋放的效果,使得該智能納米容器也能在納米催化、防腐涂層自修復(fù)等方面獲得更好的應(yīng)用。
[Abstract]:Nanovessels are usually composite materials that are nano-sized and can store and release the target material. Mesoporous silica nanospheres (MSNs) have been widely used in biomedical applications due to their advantages of large encapsulation, easy surface modification and biological nontoxicity. Especially for mesoporous silica nanovessels with stimulative response, the nanovessels can respond to the stimuli by external stimulation and realize the loading and releasing of guest molecules. The common external stimuli are pH, temperature and redox reaction, among which pH is the most widely studied. Therefore, the preparation of acidic stimulus-responsive mesoporous silica nanovessels and the controlled acidic release of drug molecules were studied in order to construct an intelligent drug carrier for acidic stimuli response. The main work is as follows. Mesoporous silica nanospheres (MSNs) prepared by S tober method were used as nano-containers. Rhodamine B, an adsorbed drug molecule, was used for the first time to bind amino-pcyclodextrin to the mesoporous site of the nano-container to block Rhodamine B with the new substance 2- (2-bromo-ethoxy) -2- (2-azido-ethoxy) propane. Action of the acid smart nano container. The morphology and structure of MSNs were characterized by scanning transmission electron microscopy (SEM / TEM), X-ray diffraction (XRD) and specific surface area analysis (BET), and the surface modification process of MSNs was verified by solid nuclear magnetic resonance (SS NMR), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). Fluorescence spectrophotometer (RF-5201) was used to monitor the release kinetics curve of the smart nano-vessel under different acidic pH, and the drug load of the smart nano-vessel was 15.23 mg / g by UV-Vis. The experimental results show that the intelligent nanovessel with Ketal chain as the stimulus response unit has achieved zero release under neutral conditions. The effect of a large amount of release (lysosome mimicking anticancer target and slow release of pH 4 to 5) under acidic conditions has a good prospect in the field of tumor cell targeting. At the same time, due to its effect of "zero release" in neutral condition and large amount of release in acidic condition, the intelligent nano-vessel can also be better applied in nano-catalysis and self-repair of anticorrosive coating.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：TQ460.1;TB383.1

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