發(fā)布時間：2018-06-04 09:27

  本文選題：熒光碳點 + 摻雜�。� 參考：《南京理工大學(xué)》2017年碩士論文

【摘要】：熒光碳點是一種小于10nm的零維新興碳材料,由于優(yōu)異并可調(diào)的熒光性質(zhì)、良好的生物相容性,在催化、生物成像、藥物遞送、傳感和基因轉(zhuǎn)染等方面具有諸多應(yīng)用。在此背景下我們制備了兩種熒光碳點,分別為氮、氯共摻雜的碳量子點(N,Cl-CQDs)以及聚乙烯亞胺(PEI)修飾的石墨烯量子點(GQDs-PEI)并分別研究了相關(guān)應(yīng)用。熒光碳點的低量子產(chǎn)率限制了在一些方面的應(yīng)用,針對這一問題本文提出通過元素摻雜的方式提高熒光量子產(chǎn)率。首先以氨基葡萄糖鹽酸鹽為唯一前驅(qū)體,既作為碳源又作為氮、氯元素的摻雜劑,制備出氮、氯雙摻雜的熒光碳量子點。通過對反應(yīng)時間和反應(yīng)溫度的優(yōu)化,制備了熒光量子產(chǎn)率高達16.8%的藍色熒光碳量子點。而后使用N,Cl-CQDs對金屬離子進行檢測,實驗發(fā)現(xiàn)N,Cl-CQDs能特異性檢測Fe3+。同時N,Cl-CQDs對低濃度的Fe3+(0-2 μM)仍具有良好的檢測效果,檢測限達到0.167 μM(R2=0.997),因此在制備傳感器方面有杰出的應(yīng)用前景。聚乙烯亞胺作為一種非病毒基因載體,具有高效轉(zhuǎn)染的效果,然而其較高的毒性也限制了作為基因載體的可能。在本文中我們通過�；磻�(yīng)將石墨烯量子點(GQDs)表面的羧基與PEI的氨基相連接,制備了質(zhì)量比為1:1,1:3和1:5的GQDs-PEI復(fù)合物并作為一種新型基因轉(zhuǎn)染載體。分別使用COS7細胞以及A549細胞進行基因轉(zhuǎn)染實驗,發(fā)現(xiàn)當GQDs與PEI-1800質(zhì)量比為1:3時具備最優(yōu)的轉(zhuǎn)染效果,轉(zhuǎn)染效率分別為51%與43%,實驗效果優(yōu)于非病毒基因轉(zhuǎn)染載體金標準PEI-25KDa的45%和35%。同時使用MTT實驗測定了GQDs-PEI-1800(1:3)在COS7細胞中的毒性,發(fā)現(xiàn)當GQDs-PEI-1800復(fù)合物濃度達到30 μg/mL時細胞存活率仍高于85%,優(yōu)于相同濃度下PEI-25KDa的細胞存活率(5%)。研究結(jié)果表明GQDs-PEI有希望發(fā)展成為一種高效低毒的基因轉(zhuǎn)染載體。
[Abstract]:Fluorescent carbon spot is a kind of zero-dimensional carbon material which is smaller than 10nm. It has many applications in catalysis, bioimaging, drug delivery, sensing and gene transfection due to its excellent and adjustable fluorescence properties and good biocompatibility. In this context, we have prepared two kinds of fluorescent carbon dots, namely nitrogen, chlorine co-doped carbon quantum dots (NCl-CQDs) and polyvinylene-imide (PEI) modified graphene quantum dots (GQDs-PEI), and studied their applications. The low quantum yield of fluorescent carbon dots limits the application in some aspects. In this paper, we propose to improve the fluorescence quantum yield by doping elements. Firstly, fluorocarbon quantum dots doped with nitrogen and chlorine were prepared by using glucosamine hydrochloride as the sole precursor, both as carbon source and as a dopant of nitrogen and chlorine. The blue fluorescent carbon quantum dots with 16.8% fluorescence quantum yield were prepared by optimizing the reaction time and reaction temperature. Then the metal ions were detected by NCCl-CQDs. It was found that NCQDs could specifically detect Fe3. At the same time, NCQDs still have a good detection effect on low concentration Fe3 (0-2 渭 M), and the detection limit is 0.167 渭 m ~ (2) C ~ (2 +) 0.997N, so it has an outstanding application prospect in the preparation of sensors. As a non-viral gene vector, polyimide has high transfection efficiency. However, its high toxicity also limits the possibility of being a gene vector. In this paper, the carboxyl groups on the surface of graphene quantum dots (GQDs) were linked to the amino groups of PEI by acylation reaction. The GQDs-PEI complexes with mass ratio of 1: 1: 1: 3 and 1:5 were prepared and used as a novel gene transfection vector. COS7 cells and A549 cells were used to carry out gene transfection experiments. The results showed that when the mass ratio of GQDs to PEI-1800 was 1:3, the transfection efficiency was 51% and 43%, respectively, which was better than that of gold standard PEI-25KDa (45% and 35%). The toxicity of GQDs-PEI-1800 1: 3) in COS7 cells was determined by MTT assay. It was found that when the concentration of GQDs-PEI-1800 complex reached 30 渭 g/mL, the cell survival rate was still higher than that of 85 cells, which was better than that of PEI-25KDa at the same concentration. The results suggest that GQDs-PEI may develop into a highly efficient and low toxic gene transfection vector.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O613.71;TB383.1

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