本文選題：氧化鐵納米粒子 + 高溫熱分解　； 參考：《桂林理工大學》2015年碩士論文

【摘要】：本文選用的鐵源為乙酰丙酮鐵(Fe(acac)3),通過高溫熱分解法制備超順磁性氧化鐵納米粒子(SPIONs)。利用X射線衍射分析(XRD)和透射電子顯微鏡(TEM)對樣品的晶相結(jié)構及形貌進行表征,通過傅里葉紅外光譜儀對樣品的表面修飾的有機物層進行分析,使用馬爾文激光粒度儀測量樣品的zeta電位值和水合動力學粒徑,樣品的磁性能通過SQUID來分析,采用感應耦合等離子體光譜儀進行測定Fe的濃度,通過磁共振成像儀分析樣品的在昆明(KM)小鼠腦部的磁共振成像(MRI)效果。主要的研究內(nèi)容如下:以PEG為溶劑,聚乙烯亞胺(PEI)為添加劑制備的PEG和PEI共同修飾的SPIONs(PEG/PEI-SPIONs)。研究結(jié)果表明所合成的PEG/PEI-SPIONs的粒徑為10.3±1.9 nm。經(jīng)紅外光譜分析表明,PEG和PEI均包覆在氧化鐵納米粒子表面,其具有較高的穩(wěn)定性和超順磁性,飽和磁化強度為50.4 emu/g,能夠在水中保持3個月以上的穩(wěn)定性,同時也能在磷酸緩溶液(PBS)中保持較好的穩(wěn)定性,PEG/PEI-SPIONs在KM小鼠腦部具有良好的MRI造影效果。將PEG/PEI-SPIONs與吐溫80(Tween 80)進行進一步接枝,接枝后得到PEG/PEI/Tween 80-SPIONs,其水合動力學粒徑由21.0 nm變?yōu)?4.0 nm,zeta電位值由35.0 mV變?yōu)?9.0 mV,經(jīng)紅外分析表明,PEG、PEI及Tween 80共同包裹在氧化鐵納米粒子表面,其具有很高的穩(wěn)定性及超順磁性,能夠在水和PBS中保持較好的穩(wěn)定性,PEG/PEI/Tween 80-SPIONs在KM小鼠腦部具有一定的MRI造影效果。將合成的PEG/PEI-SPIONs進一步包覆聚天冬氨酸(PASP),包覆PASP后得到PEG/PEI/PASP-SPIONs,其水合動力學粒徑由21 nm變?yōu)?3.4 nm,zeta電位值由35 mV變?yōu)?20.4 mV,紅外分析表明PEG、PEI及PASP共同修飾在氧化鐵納米粒子表面,其具有很高的穩(wěn)定性及超順磁性,PEG/PEI/PASP-SPIONs在KM小鼠腦部的磁共振成像具有一定的造影效果。以PEG為溶劑,0和0.3 g PVP為添加劑制備的PEG和PEG/PVP修飾的SPIONs(PEG-SPIONs、PEG/PVP-SPIONs)。進一步接枝N-(β-氨乙基-β-氨丙基)甲基二甲氧基硅烷(AEAPS)或馬來酸酐(Mal),接枝Mal后PEG-SPIONs的再分別與轉(zhuǎn)鐵蛋白(Tf)、多肽(Angiopep-2,ANG)進行連接,成功制備得到由PEG/Mal/Tf、PEG/Mal/ANG修飾的SPIONs(PEG/Mal/Tf-SPIONs、PEG/Mal/ANGSPIONs)。
[Abstract]:In this paper, the superparamagnetic ferric oxide nanoparticles (SPION) were prepared by high temperature thermal decomposition of Tie Yuan. The crystal structure and morphology of the samples were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The surface modified organic layer was analyzed by Fourier transform infrared spectroscopy (FTIR). The zeta potential and the particle size of hydration kinetics were measured by Ma Erwen laser particle size analyzer. The magnetic properties of the samples were analyzed by SQUID, and the concentration of Fe was determined by inductively coupled plasma spectrometer. Magnetic resonance imaging (MRI) was used to analyze the effect of magnetic resonance imaging (MRI) in the brain of Kunming KM mice. The main research contents are as follows: SPIONs / PEI-SPIONs modified by PEG and PEI were prepared by using PEG as solvent and polyethyleneimine (PEI) as additive. The results show that the particle size of the synthesized PEG/PEI-SPIONs is 10.3 鹵1.9 nm. The infrared spectrum analysis shows that both PEG and PEI are coated on the surface of iron oxide nanoparticles, which have high stability and superparamagnetism, and the saturation magnetization is 50.4 emu / g, which can keep the stability in water for more than 3 months. At the same time, the stability of PEG / PEI-SPIONs in the brain of km mice was better than that of PBSs in slow solution of phosphoric acid. The results showed that PEG / PEI-SPIONs had a good effect on brain MRI in km mice. PEG/PEI-SPIONs was grafted with Twain 80(Tween 80), and PEG/PEI/Tween 80-SPIONs were grafted. The particle size of PEG/PEI/Tween 80-SPIONs was changed from 21.0 nm to 24.0 nm from 35.0 MV to 19.0 MV. Ir analysis showed that PEG/PEI/Tween 80-SPIONs were encapsulated on the surface of iron oxide nanoparticles. It has high stability and superparamagnetism, and can maintain good stability in water and PBS. PEG / PEI / Tween 80-SPIONs has a certain effect of MRI imaging in brain of km mice. The synthesized PEG/PEI-SPIONs was further coated with polyaspartic acid (PASPN) and coated with PASP to obtain PEG / PEI / PASP-SPIONs. The particle size of PEG / PEI / PASP-SPIONs changed from 21 nm to 23.4 nm Zeta potential from 35 MV to -20.4 MV. Ir analysis showed that PEGN PEI and PASP were co-modified on the surface of iron oxide nanoparticles. It has high stability and magnetic resonance imaging of PEG / PEI / PASP-SPIONs in km mice. SPIONs modified by PEG and PEG/PVP were prepared with PEG as solvent 0 and 0. 3 g PVP as additives. Further grafted N-( 尾 -aminoethyl- 尾 -aminopropyl) methyl dimethoxysilane (AEAPS) or maleic anhydride (maleic anhydride), and then grafted with Mal, the PEG-SPIONs was connected with transferrin (TfN) and polypeptide Angiopep-2ANGseparately.
【學位授予單位】：桂林理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1;TQ138.11

【參考文獻】

相關期刊論文 前3條

1 王軍;張寶林;趙方圓;涂志江;王茗;;聚乙二醇/聚乙烯亞胺修飾的超順磁性氧化鐵磁共振造影劑的性能[J];材料研究學報;2013年05期

2 董翔;常庚;;Angiopep-2與轉(zhuǎn)鐵蛋白共修飾脂質(zhì)體跨血腦屏障的研究[J];第二軍醫(yī)大學學報;2014年10期

3 鄢國平,卓仁禧;磁共振成像造影劑的研究進展[J];科學通報;2001年07期

，

本文編號：1865363