發(fā)布時(shí)間：2018-05-09 04:11

  本文選題：聚縮水甘油 + 磁性納米粒子　； 參考：《天津理工大學(xué)》2015年碩士論文

【摘要】：磁性納米四氧化三鐵具有特殊的理化性質(zhì),因此有關(guān)其制備和應(yīng)用成為納米材料領(lǐng)域日漸關(guān)注的一個(gè)研究熱點(diǎn)。超支化聚縮水甘油接枝的磁性Fe3O4納米粒子(HPG-grafted MNPs)由于其杰出的生物惰性和表面可修飾性已被廣泛研究,本論文著重對(duì)其合成方法和其作為一種新的磁共振成像(MRI)造影劑的潛在醫(yī)學(xué)應(yīng)用等方面進(jìn)行了探索。在本論文中,我們通過一鍋法分別以乙酰丙酮鐵,草酸鐵為前體成功的合成出HPG-grafted MNPs。所合成的HPG-grafted MNPs的粒徑可通過調(diào)節(jié)其前體的濃度進(jìn)行調(diào)節(jié)。HPG-grafted MNPs的表面化學(xué)態(tài)和晶核形態(tài)分別進(jìn)行了X射線光電子能譜(XPS)和X射線衍射(XRD)表征。使用凝膠色譜(GPC),熱重分析(TGA),核磁共振(1 H NMR)對(duì)HPG-grafted MNPs中表面有機(jī)組分的分子量,含量與結(jié)構(gòu)進(jìn)行表征。納米顆粒的大小及形態(tài)通過透射電子顯微鏡(TEM)進(jìn)行了表征。使用動(dòng)態(tài)光散射(DLS)對(duì)納米粒子的動(dòng)態(tài)尺寸進(jìn)行測(cè)定,結(jié)果表明HPG-grafted MNPs可高度分散于水相介質(zhì)中,例如在細(xì)胞培養(yǎng)液和血清中都有極好分散性。尤其是平均粒徑為8 nm的粒子更是可以以高達(dá)45mg/ml的濃度均勻穩(wěn)定地分散在水相介質(zhì)中。體外細(xì)胞毒性實(shí)驗(yàn)(MTT)表明HPG-grafted MNPs對(duì)于巨噬細(xì)胞和3T3成纖維細(xì)胞不具有顯著毒性。隨后使用振動(dòng)樣品磁強(qiáng)計(jì)(VSM)對(duì)HPG-grafted MNPs的磁學(xué)性質(zhì)進(jìn)行了表征,通過分析所得磁通量曲線得出結(jié)論:HPG-grafted MNPs具有較高的飽和磁通量和超順磁性。然后,我們又以HPG-grafted MNPs的粒徑為變量考察了其粒徑大小對(duì)飽和磁通量、橫向弛豫(R 2)與R 2/R1比值、細(xì)胞毒性、細(xì)胞吞噬量等實(shí)驗(yàn)結(jié)果的影響。最后,我們進(jìn)行了該納米粒子的初步的動(dòng)物體內(nèi)核磁造影實(shí)驗(yàn),觀察了HPG-grafted MNPs在白兔體內(nèi)的分布情況和造影能力。
[Abstract]:Magnetic nanocrystalline ferric oxide has special physical and chemical properties, so the preparation and application of magnetic nanocrystalline ferric oxide has become a hot research topic in the field of nanomaterials. Hyperbranched polyglycidyl grafted magnetic Fe3O4 nanoparticles have been widely studied for their outstanding biological inertia and surface modifiability. In this paper, the synthetic method and its potential medical application as a new MRI contrast agent are explored. In this thesis, we successfully synthesized HPG-grafted MNPs with iron acetylacetone and ferric oxalate as precursors by one pot method. The particle size of the synthesized HPG-grafted MNPs was characterized by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction X-ray diffraction (XRD), respectively, by adjusting the concentration of the precursor. The surface chemical state and the nucleation morphology of the prepared MNPs were characterized by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), respectively. The molecular weight, content and structure of the components on the surface of HPG-grafted MNPs were characterized by gel chromatography, thermogravimetric analysis and 1H NMR. The size and morphology of nanoparticles were characterized by TEM. Dynamic light scattering (DLS) was used to determine the dynamic size of nanoparticles. The results showed that HPG-grafted MNPs could be highly dispersed in aqueous media, such as in cell culture medium and serum. In particular, particles with an average diameter of 8 nm can be uniformly and stably dispersed in aqueous media with concentrations as high as 45mg/ml. In vitro cytotoxicity test (MTT) showed that HPG-grafted MNPs was not significantly toxic to macrophages and 3T3 fibroblasts. The magnetic properties of HPG-grafted MNPs were then characterized by vibratory sample magnetometer. The magnetic flux curves obtained from the analysis showed that the high saturation magnetic flux and superparamagnetism of the MNPs were obtained by the analysis of the magnetic flux curves. Then, the effects of the particle size of HPG-grafted MNPs on the saturation magnetic flux, the ratio of transverse relaxation R _ 2) to R 2/R1, cytotoxicity and phagocytosis were investigated. Finally, the distribution and ability of HPG-grafted MNPs in rabbits were observed by nuclear magnetic resonance imaging (NMR) in vivo of the nanoparticles.
【學(xué)位授予單位】：天津理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TQ316.343;TB383.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王慧榮;李代禧;劉珊林;紀(jì)蘭蘭;吳曉瑩;;納米超順磁性鐵氧體的制備與研究[J];材料導(dǎo)報(bào);2007年S1期

2 趙雯;盧婷利;張宏;陳濤;;核殼結(jié)構(gòu)有機(jī)/無機(jī)復(fù)合微球的制備與應(yīng)用進(jìn)展[J];材料導(dǎo)報(bào);2009年07期

3 秦潤(rùn)華,劉宏英,姜煒;磁性高分子微球在生物、醫(yī)藥領(lǐng)域的應(yīng)用[J];中國(guó)粉體技術(shù);2004年04期

4 秦昆華;馬文石;;納米Fe_3O_4磁性粒子的制備及其表面改性研究進(jìn)展[J];中國(guó)粉體技術(shù);2008年02期

5 付云芝;牟敏仁;向偉;李津如;;共沉淀法合成小粒徑單分散Fe_3O_4納米顆粒[J];廣東化工;2009年09期

6 李紅,胡道道,房喻,王明珍,高改玲;磁場(chǎng)對(duì)大分子構(gòu)象的影響研究進(jìn)展[J];高分子通報(bào);2005年05期

7 王嘯;王朝宇;邱樹毅;;固定化活性污泥磁性微球用于污水處理的研究[J];貴州大學(xué)學(xué)報(bào)(自然科學(xué)版);2009年05期

8 余躍東;鐵氧體磁性納米微粒[J];貴州教育學(xué)院學(xué)報(bào)(自然科學(xué));2004年02期

9 張偉;張邦文;袁澤明;;化學(xué)共沉淀法制備Fe_3O_4納米顆粒的研究進(jìn)展[J];內(nèi)蒙古科技大學(xué)學(xué)報(bào);2012年04期

10 于巧玲;李建平;;易更新磁性碳納米管修飾電極對(duì)白蛋白的測(cè)定[J];化工技術(shù)與開發(fā);2013年12期

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