本文選題：銅納米團簇 + 碳點��； 參考：《西南大學》2017年碩士論文

【摘要】：本論文主要以熒光探針為研究對象,然后分別以熒光金屬納米團簇和熒光碳點為信號基礎,制備了一種以多巴胺為模板合成的銅納米團簇和一種以番茄汁為碳源合成的碳點,分別考察兩種熒光納米傳感材料的熒光性能、結構以及穩(wěn)定性等。然后用多巴胺銅納米團簇建立了一種用于四環(huán)素類藥物檢測的熒光分析方法,實現(xiàn)目標檢測物的特異性敏感分析,并考察了其在實際樣品中的分析應用;另外我們還應用番茄汁碳點進行了生物成像。本論文主要研究內(nèi)容如下:(1)以多巴胺為模板,在水溶液中,以硼氫化鈉為還原劑還原硫酸銅,一步合成了具有熒光的銅納米團簇(Copper nanoclusters,Cu NCs),通過紫外-可見吸收光譜、紅外光譜、熒光光譜、高分辨透射電子顯微鏡(HR-TEM)等對其表征。所制得的Cu NCs的最佳激發(fā)和發(fā)射波長分別為425 nm和485 nm,且能夠發(fā)出明亮的藍綠色熒光。該Cu NCs直徑為2-3 nm,尺寸比較均一,且沒有團聚現(xiàn)象。當Cu NCs溶液中加入一定量的四環(huán)素類藥物(TCs)時,Cu NCs表面基團和四環(huán)素類藥物表面之間反應電位降低,導致Cu NCs的熒光強度降低。因此,利用所合成的銅簇對四環(huán)素類藥物實現(xiàn)了靈敏的檢測。首先我們用此種方法對四環(huán)素(TC)檢測進行了考查,其熒光強度的減小與TC濃度在3.6×10~(-6) mol·L~(-1)到1.0×10~(-3) mol·L~(-1)范圍內(nèi)呈線性關系,其檢測限為(3σ)9.2×10~(-7)mol·L~(-1)。然后分別又對金霉素和多西環(huán)素進行了檢測,雖然也有響應,但是靈敏度不高,檢測范圍較窄。最后,將本文所建立的方法應用于牛奶中TC的檢測分析,進一步證實了此方法的實用性。此外,我們還將此銅簇進行了熒光油墨以及加密防偽方面的應用。(2)以番茄汁作為碳源,運用了一種綠色環(huán)保、創(chuàng)新的一步合成方法制備得了碳點(CDs)。一方面合成碳點所用的碳源物質(zhì)綠色環(huán)保,另一方面所合成的碳點也具有光漂白性好、高熒光量子產(chǎn)率、穩(wěn)定性好和毒性低等優(yōu)點。CDs高分辨透射電子顯微鏡成像(HR-TEM)結果顯示其平均粒徑為3 nm,且分布均勻。隨后紅外光譜結果表明在CDs表面含有-NH,-OH,-COOH等功能基團。同時用碳點進行了細胞毒性實驗,且利用碳點優(yōu)異的熒光性能進行了生物成像。上述結果對我們深入認識碳點的性能,以及對進一步將其應用于傳感分析中都具有重要價值。
[Abstract]:In this thesis, a kind of copper nanoclusters synthesized by dopamine template and a carbon point synthesized by tomato juice as carbon source were prepared on the basis of fluorescent metal nanoclusters and fluorescent carbon spots, respectively. The fluorescence properties, structure and stability of two kinds of fluorescent nanosensors were investigated. Then a fluorescence analysis method for tetracycline drug detection was established by using dopamine copper nanoclusters to realize the specificity and sensitivity analysis of the target and its application in practical samples was investigated. In addition, we also used tomato juice carbon spots for biological imaging. The main contents of this thesis are as follows: (1) Copper nanoclusters copper nanoclusters with fluorescence were synthesized by using dopamine as template and sodium borohydride as reductant to reduce copper sulfate in aqueous solution. Fluorescence spectra and high resolution transmission electron microscopy (HR-TEM) were used to characterize them. The optimum excitation and emission wavelengths of Cu NCs are 425nm and 485nm, respectively, and can emit bright blue-green fluorescence. The diameter of Cu NCs is 2-3 nm, the size is uniform and there is no agglomeration. When a certain amount of tetracycline was added to Cu NCs solution, the reaction potential between the surface group of Cu NCs and the surface of tetracycline was decreased, which resulted in the decrease of fluorescence intensity of Cu NCs. Therefore, the synthesized copper clusters were used to detect tetracycline drugs sensitively. First of all, we used this method to detect tetracycline (TCC). The decrease of fluorescence intensity is linear with TC concentration in the range of 3.6 脳 10 ~ (-6) mol / L ~ (-1) to 1.0 脳 10 ~ (-3) mol / L ~ (-1), and the detection limit is 3 蟽 ~ 9.2 脳 10~(-7)mol ~ (-1) 路L ~ (-1). Then chlortetracycline and doxycycline were detected respectively. Although there was a response, the sensitivity was not high and the detection range was narrow. Finally, the proposed method is applied to the detection and analysis of TC in milk, which further proves the practicability of the method. In addition, the copper cluster was used in fluorescent ink and encryption anti-counterfeiting applications. The tomato juice was used as carbon source, and a green and innovative one-step synthesis method was used to prepare the carbon dot CDsN. On the one hand, the carbon sources used in the synthesis of carbon dots are green and environmentally friendly; on the other hand, the carbon dots synthesized also have good photobleaching properties and high fluorescence quantum yields. The results of HR-TEM imaging show that the average particle size of CDs is 3 nm and its distribution is uniform. The infrared spectra show that there are some functional groups such as -NH _ (-) -OH _ (-COOH) on the surface of CDs. At the same time, the cytotoxicity test was carried out with carbon spots, and the excellent fluorescence properties of carbon spots were used for biologic imaging. These results are of great value for us to understand the properties of carbon points and to further apply them to sensor analysis.
【學位授予單位】：西南大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;TB383.1

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本文編號：1806154