本文選題：內(nèi)皮祖捕獲 + 干細(xì)胞��； 參考：《中國科學(xué):技術(shù)科學(xué)》2017年05期

【摘要】：構(gòu)建內(nèi)皮祖細(xì)胞捕獲支架是目前心血管疾病治療的一個研究熱點.利用磁性納米粒子捕獲內(nèi)皮祖細(xì)胞也越來越受到關(guān)注,但是單純的磁性納米粒子和磁場源地作用相對微弱,本研究引入鐵支架作為另一磁響應(yīng)源,共同作用來提高納米粒子表面捕獲率.首先利用CD34抗體構(gòu)建了一種定向捕獲CD34陽性細(xì)胞的功能型磁性納米粒子,再利用外加磁場將其歸巢到鐵支架表面.FTIR和TEM結(jié)果表明CD34抗體已成功接枝到雙羧基PEG包裹的Fe_3O_4納米粒子上,體外細(xì)胞評價證實該納米粒子不具有細(xì)胞毒性.體內(nèi)動物實驗結(jié)果表明該Fe_3O_4-PEG@CD34納米粒子能夠特異性識別捕獲CD34陽性細(xì)胞,并且在外加磁場的作用下能夠?qū)⑵淇焖贇w巢到鐵支架表面,這為實現(xiàn)支架表面快速內(nèi)皮化提供了可能.
[Abstract]:The construction of endothelial progenitor cell capture stent is a hot topic in the treatment of cardiovascular disease. The capture of endothelial progenitor cells by magnetic nanoparticles has attracted more and more attention, but the effect of magnetic nanoparticles and magnetic field source is relatively weak. In this study, iron scaffolds were introduced as another magnetic response source. The surface trapping rate of nanoparticles can be improved by interaction. First, a functional magnetic nanoparticles was constructed by using CD34 antibody to capture CD34 positive cells. The results of homing to the surface of iron scaffold. FTIR and TEM showed that CD34 antibody had been successfully grafted onto the Fe_3O_4 nanoparticles coated with dicarboxylic PEG. The cell evaluation in vitro confirmed that the nanoparticles were not cytotoxic. The results of animal experiments in vivo show that the Fe_3O_4-PEG@CD34 nanoparticles can specifically recognize and capture CD34 positive cells, and can quickly homing them to the surface of the iron scaffold under the action of external magnetic field, which provides the possibility for the rapid endothelialization of the scaffold surface.
【作者單位】： 西南交通大學(xué)材料先進技術(shù)教育部重點實驗室;西南交通大學(xué)生命科學(xué)與工程學(xué)院;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號:81271701)資助項目
【分類號】：R54;TB383.1
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本文編號：1779434