本文選題：銅納米簇 + 金納米簇�。� 參考：《湘潭大學(xué)》2017年碩士論文

【摘要】：金屬納米簇是一種新型的納米材料。它具有尺寸小、穩(wěn)定性好、斯托克斯位移大、生物相容性好等優(yōu)點(diǎn),近年來被廣泛地應(yīng)用于分析化學(xué)。ABTS是一種過氧化氫酶的底物,ABTS/ABTS+的氧化還原電位為0.68 V,容易發(fā)生電子轉(zhuǎn)移,生成穩(wěn)定的綠色自由基能進(jìn)行可視化檢測(cè)方面,被作為光學(xué)探針廣泛應(yīng)用于生物、化學(xué)等各個(gè)領(lǐng)域。適配體是一小段經(jīng)體外篩選得到的寡核苷酸序列,能與相應(yīng)的目標(biāo)物質(zhì)進(jìn)行高度特異性結(jié)合,同時(shí)可作為模板合成金屬納米簇。本文主要完成了金屬納米簇的制備、表征及其分析應(yīng)用等實(shí)驗(yàn)內(nèi)容。具體概述如下:(1)合成了以二硫蘇糖醇(DTT)為模板的銅納米簇和檸檬酸穩(wěn)定的納米銀,分別采用紫外-可見分光光度法、熒光光譜法等方法進(jìn)行了表征。通過內(nèi)濾效應(yīng),納米銀能使銅納米簇的熒光發(fā)生猝滅,加入三聚氰胺后,由于三聚氰胺使納米銀團(tuán)聚,內(nèi)濾光作用被破壞,銅納米簇?zé)晒饣謴?fù)。實(shí)驗(yàn)結(jié)果表明,三聚氰胺濃度在4-28μM范圍內(nèi),體系的熒光恢復(fù)程度與三聚氰胺的濃度存在良好的線性關(guān)系,檢出限為0.98μM,實(shí)際樣品(牛奶)的加標(biāo)回收率為96.5%-103.8%。(2)合成了以氯霉素適配體為模板的銅納米簇,基于合成的DNA銅納米簇在核酸外切酶Ⅰ(Exo Ⅰ)的作用下導(dǎo)致模板破壞而熒光猝滅,同時(shí)二價(jià)銅離子在過氧化氫以及肌酸肝的共同作用下會(huì)使ABTS被氧化成綠色的ABTS+,則可通過熒光、紫外以及目視檢測(cè)Exo Ⅰ。實(shí)驗(yàn)結(jié)果表明,當(dāng)Exo Ⅰ的濃度在0.1-10 U mL-1范圍內(nèi),體系的熒光強(qiáng)度與Exo Ⅰ濃度存在著很好的線性關(guān)系,檢出限為0.08 U mL-1,實(shí)際樣品(血清)加標(biāo)回收率為92.4%-103.7%。(3)合成了以DNA為模板的金納米簇,合成的DNA金納米簇在過氧化氫和亞鐵離子的芬頓反應(yīng)作用下導(dǎo)致模板破壞而熒光猝滅,同時(shí)三價(jià)金離子會(huì)使ABTS被氧化成綠色的ABTS+,則可通過熒光、紫外以及目視檢測(cè)羥基自由基。實(shí)驗(yàn)結(jié)果表明,當(dāng)過氧化氫濃度在2-15μM范圍內(nèi),體系的熒光相對(duì)強(qiáng)度F0-F與過氧化氫濃度存在著很好的線性關(guān)系。檢出限為1.5μM,對(duì)于實(shí)際樣品(血清)的加標(biāo)回收率為90.6%-108.6%。
[Abstract]:Metal nanoclusters are a new type of nanomaterials.It has the advantages of small size, good stability, large Stokes shift and good biocompatibility. In recent years, it has been widely used in analytical chemistry. ABTS is a kind of catalase substrate. The redox potential of ABTS / ABTS is 0.68 V, which makes it easy to transfer electrons.The generation of stable green free radicals for visual detection is widely used as optical probes in biological, chemical and other fields.Aptamer is a small sequence of oligonucleotides screened in vitro, which can bind to the target material with high specificity and can be used as a template for the synthesis of metal nanoclusters.In this paper, the preparation, characterization and application of metal nanoclusters were studied.The copper nanoclusters and citric acid stabilized silver nanoparticles were synthesized and characterized by UV-Vis spectrophotometry and fluorescence spectrometry respectively.Through internal filtration, nano-silver can quench the fluorescence of copper nanoclusters. After the addition of melamine, the silver nanoparticles are agglomerated by melamine, the internal filter is destroyed, and the fluorescence of copper nanoclusters recovers.The experimental results show that there is a good linear relationship between the fluorescence recovery degree of the system and the concentration of melamine in the range of 4-28 渭 M.Copper nanoclusters with chloramphenicol aptamer as template were synthesized with the detection limit of 0.98 渭 m and the standard recovery of actual samples (milk) of 96.5- 103.8. the results showed that the synthesized DNA copper nanoclusters caused template destruction and fluorescence quenching under the action of nucleic acid exonuclease 鈪，

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