本文選題：碳點(diǎn) + 金黃色葡萄球菌�。� 參考：《湖南科技大學(xué)》2017年碩士論文

【摘要】：作為新型碳納米材料,碳點(diǎn)(CDs)具有優(yōu)良性能,被廣泛地應(yīng)用于多個(gè)領(lǐng)域。CDs的粒徑普遍在3~5 nm之間,表面含有各類功能化基團(tuán),且可通過(guò)改變功能化集團(tuán)來(lái)調(diào)整CDs的性質(zhì)。相對(duì)于傳統(tǒng)的半導(dǎo)體量子點(diǎn)來(lái)說(shuō),CDs具備更好的光學(xué)性能、生物相容性和環(huán)境友好性,能夠應(yīng)用于生物成像、納米傳感、光催化、光電子器件等范圍。本文基于細(xì)菌自身具有較均勻的特征,以其為碳源,利用水熱法或微波輔助制備出性能優(yōu)良的熒光CDs,并用于離子檢測(cè)等各個(gè)方面:(1)本文以金色葡萄糖球菌為碳源,結(jié)合水熱法合成技術(shù)成功制備了均勻性較好的CDs,經(jīng)提純后得到具備熒光特性的CDs溶液。獲得的CDs在350 nm的激發(fā)下,于440nm處有一熒光發(fā)射峰。根據(jù)內(nèi)濾效應(yīng),在酸性條件下,CDs可以用來(lái)檢測(cè)鹽酸小檗堿。當(dāng)CDs溶液與鹽酸小檗堿溶液反應(yīng)后,CDs溶液的熒光會(huì)發(fā)生猝滅,猝滅值與鹽酸小檗堿的溶液濃度呈線性關(guān)系(線性方程為(35)I=3.79×106c+16.02,r=0.9972),線性范圍為1.0×10-7-7.5×10-5 mol/L,檢測(cè)限為7.03×10-8(3σ/k)�；诖司�性關(guān)系,能夠檢測(cè)鹽酸黃連素片樣品中的鹽酸小檗堿含量。(2)本文以酵母細(xì)菌為主碳源,乙二胺為輔助碳源,微波快速合成CDs溶液,獲得有熒光特性的CDs溶液;獲得的CDs在390 nm的激發(fā)下,于460 nm處有一熒光發(fā)射峰。該CDs能夠用于溶液pH的檢測(cè)。CDs溶液與不同pH的溶液反應(yīng)后,CDs溶液的熒光強(qiáng)度值與溶液的pH之間呈線性關(guān)系(IF=259.90-12.61 pH,r=0.9936),檢測(cè)的線性范圍為線性范圍為1.81~11.82。(3)本文以羧甲基葡聚糖為定穩(wěn)劑和還原劑,紫外燈照射[Ag(NH3)2]OH溶液獲得一種新的溶于水溶性熒光銀納米簇;并對(duì)其大小和形貌進(jìn)行了TEM、HRTEM、熒光和紫外光譜表征�；阢y納米簇與半胱氨酸之間的相互作用,導(dǎo)致銀納米簇?zé)晒忖?猝滅值與半胱氨酸的溶液濃度呈線性關(guān)系(線性方程(35)I=4.25×109c+36.84,r=0.9967),線性檢測(cè)范圍為5.0×10-10 1.0×10-7 mol/L,檢測(cè)限為3.16×10-10(3?/k)�；诖司�性關(guān)系,建立了一種可用于半胱氨酸檢測(cè)的熒光傳感器。
[Abstract]:As a new carbon nanomaterials, carbon dots (CDs) have been widely used in many fields. The diameters of CDs are generally between 3nm and 5nm. The surface contains various functional groups, and the properties of CDs can be adjusted by changing the functionalized groups.Compared with traditional semiconductor quantum dots, CDs have better optical properties, biocompatibility and environmental friendliness. They can be used in biological imaging, nanosensor, photocatalysis, optoelectronic devices and so on.In this paper, based on the homogeneous characteristics of bacteria, the fluorescent CDS with excellent properties were prepared by hydrothermal method or microwave-assisted method using them as carbon source, and they were used in ion detection and other aspects. In this paper, Glucococcus aureus was used as carbon source, and Glucococcus aureus was used as carbon source.CDs with good homogeneity was successfully prepared by hydrothermal synthesis. After purification, the CDs solution with fluorescence characteristics was obtained.Under the excitation of 350nm, the obtained CDs has a fluorescence emission peak at 440nm.According to the internal filtration effect, CDs can be used to detect berberine hydrochloride under acidic conditions.After the reaction of CDs solution with berberine hydrochloride solution, the fluorescence of CDs solution will be quenched. The quenching value is linear with the concentration of berberine hydrochloride solution (linear equation is 3.79 脳 10 ~ (-6) C ~ (16.02C) ~ (0.9972)), the linear range is 1.0 脳 10 ~ (-7) -7.5 脳 10 ~ (-5) mol 路L ~ (-1), the detection limit is 7.03 脳 10 ~ (-8) ~ (-3) 蟽 路K ~ (-1).Based on this linear relationship, berberine hydrochloride content in berberine hydrochloride tablets can be determined. In this paper, yeast bacteria as the main carbon source, ethylenediamine as the auxiliary carbon source, microwave rapid synthesis of CDs solution, to obtain fluorescence characteristics of CDs solution;Under the excitation of 390nm, the obtained CDs has a fluorescence emission peak at 460nm.The CDs can be used to detect pH of solution. After reaction between CDs solution and solution with different pH, there is a linear relationship between fluorescence intensity value of CDs solution and pH value of solution.Sugar is stabilizer and reductant,A novel water-soluble fluorescent silver nanocluster was obtained by ultraviolet lamp irradiation with [Ag(NH3)2] OH solution, and its size and morphology were characterized by TEM HRTEM, fluorescence and UV spectra.Based on the interaction between silver nanoclusters and cysteine, the fluorescence quenching of silver nanoclusters was induced. The quenching value was linearly related to the concentration of cysteine solution (linear equation = 3.35 脳 10 ~ (-9) c ~ 36.84C ~ (0.9967)). The linear detection range was 5.0 脳 10 ~ (-10) ~ (-10) ~ (-1) mol 路L ~ (-1), and the detection limit was 3.16 脳 10 ~ (-10) ~ (3) C ~ (-1) / kg ~ (-1).Based on the linear relationship, a fluorescence sensor for detection of cysteine was established.
【學(xué)位授予單位】：湖南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB383.1;O657.3

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本文編號(hào)：1745911