本文選題：碳量子點　切入點：氮摻雜　出處：《華僑大學(xué)》2017年碩士論文

【摘要】：碳量子點(CQDs),具有了優(yōu)越的光電性能、良好的水溶性和生物相容性,并且制備方法簡單,使其在化學(xué)傳感、生物成像等方面具有巨大的應(yīng)用潛力。本文基于它的卓越性能,從制備出發(fā),通過不同前驅(qū)體,以摻雜雜原子的方式制備獲得不同性能的CQDs,并探索該些材料在熒光傳感和細(xì)胞成像方面的應(yīng)用。具體研究內(nèi)容如下:1、本文利用微波法制備得到氮摻雜碳量子點(NCQDs)。通過透射電鏡(TEM)、X射線能譜儀(EDS)、光電子能譜儀(XPS)和傅里葉紅外光譜儀(FTIR)對其形貌、結(jié)構(gòu)和組分進(jìn)行表征,結(jié)果表明所純化出的NCQDs,平均粒徑約為4.0 nm,表面含有豐富的含氧含氮基團(tuán),因此是一種氮摻雜的碳納米材料。通過紫外分光光度計和熒光分光光度計對NCQDs光學(xué)性能進(jìn)行表征,結(jié)果顯示NCQDs的紫外吸收在245 nm和339 nm,其熒光發(fā)射峰具有波長依賴性,最大熒光激發(fā)和發(fā)射峰分別為383 nm和457 nm。此外,該材料的熒光發(fā)射峰還具有pH依賴性和良好的環(huán)境穩(wěn)定性。由于3,5-二硝基水楊酸對NCQDs熒光表現(xiàn)出強(qiáng)烈的熒光猝滅響應(yīng),因此,構(gòu)建了基于NCQDs熒光探針的3,5-二硝基水楊酸的熒光檢測方法,并對該檢測體系進(jìn)行優(yōu)化。結(jié)果表明,3,5-二硝基水楊酸對NCQDs猝滅的程度與其濃度在1μmol/L~100μmol/L內(nèi)呈良好的線性關(guān)系(R2=0.9976),檢測限為0.68μmol/L(S/N=3)。同時,初步討論3,5-二硝基水楊酸對NCQDs熒光的猝滅機(jī)制。2、在前一個實驗的基礎(chǔ)上,我們改變前驅(qū)體,以檸檬酸和半胱氨酸為前驅(qū)體,用微波法制備得到高熒光量子產(chǎn)率的氮硫共摻雜的碳量子點(NSCQDs)。用TEM對其形貌進(jìn)行表征,結(jié)果表明NSCQDs為近球形,其平均粒徑約為2.2nm。FTIR結(jié)果表面該材料表面含有含氧、含氮、含硫基團(tuán),證明其屬于氮硫雙摻雜的碳納米材料。光學(xué)表征結(jié)果顯示,該材料在紫外可見范圍內(nèi)有三個吸收峰,分別處于212、244和348 nm;其最佳熒光激發(fā)和發(fā)射波長分別為356 nm和423 nm,熒光量子產(chǎn)率可達(dá)61.41%,并且熒光發(fā)射波長不會隨著激發(fā)波長的改變而移動,但是具有濃度依賴性和一定范圍的pH穩(wěn)定性。此外,該材料的發(fā)光性能同樣具有良好的環(huán)境穩(wěn)定性和生物相容性,因此,我們探索了該材料在細(xì)胞成像和熒光“油墨”筆方面的應(yīng)用。
[Abstract]:Carbon quantum dots (CQDs), with excellent optoelectronic properties, good water solubility and biocompatibility, and the preparation method is simple, the chemical sensing, have great potential applications in bio imaging and so on. In this paper, based on its superior performance, from the preparation of the precursor, to doped heteroatom to prepare and obtain the different performance of CQDs, and to explore the application of these materials in fluorescence sensing and cell imaging. The specific contents are as follows: 1, using microwave prepared nitrogen doped carbon quantum dots (NCQDs). By transmission electron microscopy (TEM), X ray spectrometer (EDS), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectrometer (FTIR) on the morphology, structure and composition were characterized. The results show that the purified NCQDs, the average particle size is about 4 nm, the surface rich in oxygen nitrogen group, it is a carbon nano material doped with nitrogen Material. Spectrophotometer was used to characterize the optical properties of NCQDs by UV spectrophotometer and fluorescence results show that the UV absorption of NCQDs at 245 nm and 339 nm, the fluorescence emission peak with the wavelength dependence of the maximum fluorescence excitation and emission peaks were 383 nm and 457 nm. in addition, the fluorescent material the emission peak is pH dependent and good environmental stability. Because of the 3,5- two nitro salicylic acid on NCQDs fluorescence showed strong fluorescence quenching response, therefore, constructs the fluorescence detection method of NCQDs fluorescent probe 3,5- two nitro salicylic acid based, and the detection system were optimized. The results showed that 3,5- two nitro salicylic acid degree the NCQDs quenching of a good linear relationship with its concentration in 1 mol/L~100 mol/L (R2=0.9976), the detection limit was 0.68 mol/L (S/N=3). At the same time, a preliminary discussion of 3,5- two nitro salicylic acid quenching mechanism of the fluorescence of NCQDs.2 In the previous experiment, based on, we change the precursor with citric acid and cysteine as the precursor, carbon quantum dots were prepared by microwave with high fluorescence quantum yield of sulphur and nitrogen Co doped (NSCQDs). The morphology was characterized by TEM, the results showed that NSCQDs was nearly spherical, the average the size is about 2.2nm.FTIR the surface of the material containing oxygen, nitrogen, sulfur groups, carbon nano materials that it belongs to the nitrogen and sulfur doped. The optical characterization results show that the material has three absorption peaks in the UV visible range, respectively in 212244 and 348 nm; the optimal excitation and emission the wavelength was 356 nm and 423 nm, the fluorescence quantum yield of up to 61.41%, and the fluorescence emission wavelength doesn't move when the excitation wavelength is changed, but pH has concentration dependent stability and a certain range. In addition, the material has the same luminous performance Good environmental stability and biocompatibility, therefore, we have explored the application of the material in cell imaging and fluorescence "ink" pen.

【學(xué)位授予單位】：華僑大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O613.71;TB383.1

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本文編號：1698001