本文選題：靜電紡絲　切入點(diǎn)：核殼結(jié)構(gòu)　出處：《浙江理工大學(xué)》2017年碩士論文

【摘要】：1.用共軸靜電紡絲的方法將天然高分子肝素和纖維素溶解在1-乙基-3-甲基咪唑醋酸鹽(1-ethyl-3-methylimidazolium acetate,[EMIM][Ac])離子液體作為殼溶液,同時(shí)將四氧化三鐵納米顆粒(5 nm)溶解在[EMIM][Ac]離子液體作為核溶液,高壓紡絲后得到具有核殼結(jié)構(gòu)的纖維。并用SEM,TEM對(duì)其表面形貌和內(nèi)部結(jié)構(gòu)進(jìn)行了表征,用EDX元素分析確定了復(fù)合纖維材料元素的組成,用XRD對(duì)纖維素及四氧化三鐵納米顆粒的晶型物相進(jìn)行了分析,用TGA熱重分析的方法確定了核殼纖維中四氧化三鐵的含量,LC-MS液相色譜與質(zhì)譜聯(lián)用技術(shù)確定了肝素的存在,同時(shí)也對(duì)肝素、纖維素和四氧化三鐵核殼纖維進(jìn)行了Xa和IIa抗凝血活性分析,結(jié)果表明殼結(jié)構(gòu)里包含肝素的核殼纖維具有很好的抗凝血活性。2.用共軸靜電紡絲的方法將合成高分子聚己內(nèi)酯(polycaprolactone,PCL)溶于2,2,2-三氟乙醇溶劑中作為紡絲殼溶液,同時(shí)分別將40%,60%,80%(w/v)合成彈性高分子聚合物聚癸二酸丙三醇酯(poly-glycerol sebacate,PGS)溶于2,2,2-三氟乙醇溶劑中作為核溶液進(jìn)行紡絲,為了突出具有核殼結(jié)構(gòu)PCL/PGS支架材料形貌、機(jī)械強(qiáng)度等方面的特點(diǎn),將PCL和PGS溶于2,2,2-三氟乙醇溶劑的混合溶液進(jìn)行單泵紡絲作為對(duì)照。另一方面,對(duì)于具有核殼結(jié)構(gòu)的PCL/PGS纖維,由于PCL是疏水性材料,缺乏血管細(xì)胞生長(zhǎng)的附著位點(diǎn),同時(shí)作為人工血管材料要有一定的抗凝血性,我們通過(guò)化學(xué)的方法將肝素嫁接了在PCL/PGS支架表面。我們對(duì)材料的化學(xué)性能、機(jī)械性能以及生物性能進(jìn)行了表征,PCL/PGS核殼材料具有可控制的降解性能以及可調(diào)諧的機(jī)械性能。降解緩慢的PCL外殼主要作為機(jī)械支撐和結(jié)構(gòu)框架,降解快速的PGS材料則增加了材料的彈性性能,隨著PGS/PCL比例的變化,得到材料的楊氏模量變化范圍是5.6M-15.7M,極限拉伸應(yīng)力變化范圍是2.0M-2.9M,伸長(zhǎng)率的變化范圍是291.4%-906.8%,該材料在組織工程應(yīng)用方面提供了可能。
[Abstract]:1. Natural macromolecule heparin and cellulose were dissolved in 1-ethyl-3-methylimidazolium acetate1 ethyl-3-methylimidazolium acetate, [EMIM] [ac] ionic liquid as shell solution by coaxial electrospinning, and iron trioxide nanoparticles (5 nm) were dissolved in [EMIM] [ac] ionic liquid as nuclear solution. The fibers with core-shell structure were obtained by high pressure spinning. The surface morphology and internal structure of the fibers were characterized by EDX, and the composition of the composite fibers was determined by EDX elemental analysis. The crystalline phase of cellulose and iron trioxide nanoparticles was analyzed by XRD. The content of iron trioxide in core-shell fiber was determined by TGA thermogravimetric analysis. The presence of heparin was determined by LC-MS liquid chromatography and mass spectrometry. The anticoagulant activities of heparin, cellulose and ferric oxide core-shell fibers were also analyzed by Xa and IIa. The results show that the core-shell fibers containing heparin have good anticoagulant activity. The synthetic polycaprolactone polycaprolactone PCL (PCL) is dissolved as a spinning shell solution in 2C 2N 2fluoroethanol solvent by coaxial electrostatic spinning. At the same time, the elastic polymer poly-glycerol sebacate (PGSs), which was synthesized from 40 / 60 / 80 / w / v / w / / /,, respectively, was spun as a nuclear solution in the solvent of 2o 2o 2 fluoroethanol, in order to highlight the characteristics of the PCL/PGS scaffold with core-shell structure, such as morphology, mechanical strength, and so on. In contrast, PCL and PGS were spun by single pump in the mixed solution of 2H 2H 2F ethanol solvent. On the other hand, for PCL/PGS fibers with core-shell structure, because PCL was hydrophobic, there was no adhesion site for the growth of vascular cells. At the same time, as an artificial blood vessel material to have a certain degree of anticoagulant, we chemically grafted heparin on the surface of the PCL/PGS scaffold. The mechanical and biological properties of PCL / PGS core-shell materials are characterized by their controllable degradation and tunable mechanical properties. The slow-degrading PCL shells are mainly used as mechanical support and structural framework. The fast degradable PGS material increased the elastic properties of the material, with the change of PGS/PCL ratio. The Young's modulus is 5.6M-15.7m, the ultimate tensile stress is 2.0M-2.9M, and the elongation is 291.4-906.8m, which provides the possibility for the application of the material in tissue engineering.
【學(xué)位授予單位】：浙江理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TB34

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