本文選題：洋蔥伯克霍爾德氏菌　切入點(diǎn)：生物礦化　出處：《東北林業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：從山東省聊城市東昌湖水域取湖水樣品,通過富集培養(yǎng)、分離純化的方法篩選出一株產(chǎn)磁性納米顆粒的菌株,經(jīng)生理生化測定及16S rDNA測序分析結(jié)果,鑒定為洋蔥伯克霍爾德氏菌(Burkholderia cepacia),將其命名為B. cepacia TZ-11。B. cepacia TZ-1為兼性厭氧菌,在氧分壓低于1%條件下才能合成磁性納米顆粒,本研究獨(dú)創(chuàng)性采用微氧密封、120 r/min搖床培養(yǎng)的方法來培養(yǎng)B. cepacia TZ-1。B. cepacia TZ-1為桿菌,菌體大小約1-2μm×0.5-1 μm,有鞭毛,革蘭氏陰性。在平板上菌落直徑約為3-5mm、呈圓形、表面光滑、邊緣不規(guī)則、菌落濕潤呈黃綠色、半透明、有黏性。透射電鏡下可觀察到菌體細(xì)胞壁周圍有大小約60 nm致密晶體顆粒圍成一圈、菌體內(nèi)部含有3-5顆180 nm的致密晶體顆粒。采用超聲破碎方法提取純化磁性納米顆粒,透射電鏡、掃描電鏡對其進(jìn)行元素分析,結(jié)果一致,主要元素為Fe、P、O,X射線衍射分析磁性納米顆粒成分為Fe3(PO4)2·8H2O、Fe3O4,磁性納米顆粒為單斜晶體。磁性納米顆粒在H202存在條件下具有過氧化物酶類酶活性,與商品HRP進(jìn)行比較,磁性納米顆粒對TMB的親和力稍強(qiáng),且對于H202濃度、pH、溫度有極強(qiáng)的耐受性。磁性納米顆粒在H202存在條件下可以催化染料脫色,本研究選擇偶氮類染料甲基橙、蒽醌類染料酸性媒介紅、芳甲烷類染料固綠FCF、靛族染料靛紅作為研究對象,磁性納米顆粒對上述4種染料均具有較好的降解脫色效果,且磁性納米顆粒對染料脫色過程中,有極強(qiáng)的H202濃度、pH、溫度耐受性。
[Abstract]:A strain producing magnetic nanoparticles was isolated from lake water samples from Dongchang Lake, Liaocheng City, Shandong Province. The results of physiological and biochemical analysis and 16s rDNA sequencing were analyzed. It was identified as Burkholderia cepacia of Onion and named B. cepacia TZ-11.B. cepacia TZ-1 as facultative anaerobes. The magnetic nanoparticles could be synthesized only when the oxygen partial pressure was lower than 1%. In this study, the microoxygen seal 120 r/min shaker culture method was used to culture B. cepacia TZ-1.B. cepacia TZ-1. The bacteria were about 1-2 渭 m 脳 0.5-1 渭 m in size, flagellum and Gram-negative. The diameter of colony on the plate was about 3 ~ 5 mm, the surface was round, the surface was smooth, and the edge was irregular. The colony was yellowish green, translucent and sticky. Under transmission electron microscope, it was observed that there were about 60 nm dense crystal particles around the cell wall in a circle. There were 3 to 5 dense crystal particles of 180 nm in the cell. The magnetic nanoparticles were extracted and purified by ultrasonic fragmentation. The results were consistent with the results of elemental analysis by transmission electron microscope and scanning electron microscope. The main element is Fe3(PO4)2 8 H 2O O 3 O 4, and the magnetic nanoparticles are monoclinic crystals. The magnetic nanoparticles have peroxidase activity in the presence of H202, and are compared with commercial HRP. Magnetic nanoparticles have stronger affinity to TMB, and have strong tolerance to H202 pH and temperature. Magnetic nanoparticles can catalyze the decolorization of dyes in the presence of H202. In this study, azo dyes methyl orange was selected. Anthraquinone dyes, acid mordant red, aromatic methane dyes, solid green FCFs and indigo dye indirubin were studied. The magnetic nanoparticles had good degradation and decolorization effect on the above four dyes, and the magnetic nanoparticles decolorized the dyes. It has strong pH value of H 202 and temperature tolerance.
【學(xué)位授予單位】：東北林業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1

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本文編號：1647272