本文選題：電化學　切入點：生物傳感器　出處：《深圳大學》2017年碩士論文　論文類型：學位論文

【摘要】：隨著電化學生物傳感器的不斷發(fā)展,出現(xiàn)了眾多性能優(yōu)異,測試精準的電化學生物傳感器,并被運用于醫(yī)療、安全檢測、食品安全和環(huán)境保護等多個領域。由于生物傳感器具有檢測過程簡單,準確性高,靈敏度高等優(yōu)點,成為各種檢測應用的研究重點。但隨著電化學生物傳感器的不斷普及,其研究更多是考慮到用戶的需求,傳感器設備逐漸趨于微型化、便攜化。因此,近年來出現(xiàn)了印刷電極、酶膜反應器等新型傳感器。他們除了具有檢測的高效性和穩(wěn)定性外,還具有方便用戶使用與測量的優(yōu)勢。本文通過制備出柔性葡萄糖和乳酸傳感器,并制備出性能優(yōu)異的酶膜傳感器,實現(xiàn)傳感器的高效化、便攜化和微型化等目的。首先,通過在SBS薄膜中摻入PEDOT:PSS水溶液以及MWNT-OH制備出導電高分子薄膜,并作為一種傳感器電極使用,使用殼聚糖與戊二醛的交聯(lián)來固定氧化酶,制備出電化學性能良好的柔性葡萄糖生物傳感器和柔性乳酸生物傳感器。制備的柔性葡萄糖生物傳感器,其檢測線性范圍為0~6 m M,線性回歸方程為I=2.21*c+1.4455,R~2=0.996,檢測靈敏度為2.21μA/m M,檢出限為12μM(S/N=3)。制備的柔性乳酸生物傳感器,其檢測線性范圍為0.2~1.6 m M,線性回歸方程為I=7.00*c+0.5796,R~2=0.9935,檢測靈敏度為7.00μA/m M,檢出限為7.87μM(S/N=3)。這兩種傳感器均具有良好的穩(wěn)定性和抗干擾性能。其次,通過改進后的Hummers法成功制備出氧化石墨烯,并通過溶劑熱法制備出G-Pt NPs,作為電化學敏感材料制備出乳酸生物傳感器,具有良好的檢測穩(wěn)定性和抗干擾性能,其檢測線性范圍為0~1.2 m M,線性回歸方程為I=2.08*c+0.1623,R~2=0.990,檢測靈敏度為2.08μA/m M,檢出限為12.5μM(S/N=3)。同時,所制備出的柔性乳酸生物傳感器,具有良好的檢測穩(wěn)定性和重現(xiàn)性,檢測靈敏度為2.08μA/m M,通過對人體汗液進行實時檢測,發(fā)現(xiàn)該柔性乳酸傳感器可以對人體汗液進行檢測。最后,通過在PVB薄膜中加入MWNT-OH制備導電薄膜基底,修飾上氧化酶,制備出性能優(yōu)異的葡萄糖和乳酸生物傳感器。其中,使用1%PVB制備出的葡萄糖傳感器,其檢測線性范圍為0~3 m M,線性回歸方程為I=0.03*c+0.0305,R~2=0.993,檢測靈敏度為0.03μA/m M,檢出限為49.1μM(S/N=3)。加入TTF后,其檢測線性范圍增加到為0~32 m M,線性回歸方程為I=0.26*c+0.4899,R~2=0.990,檢測靈敏度為0.26μA/m M,檢出限為62.8μM(S/N=3),線性檢測濃度范圍大大提高。另外,使用1%PVB制備出的乳酸傳感器,其檢測線性范圍為0.5~1.5 m M,線性回歸方程為I=0.20*c+0.0967,R~2=0.992,檢測靈敏度為0.20μA/m M,檢出限為18.8μM(S/N=3)。加入TTF后,使用0.1%GA固定LOx制備出乳酸傳感器,其檢測線性范圍為2~5 m M,線性回歸方程為I=1.51*c+1.4956,R~2=0.991,檢測靈敏度為1.51μA/m M,檢出限為2.98μM(S/N=3),線性檢測濃度范圍有所提高。
[Abstract]:With the development of electrochemical biosensors, many electrochemical biosensors with excellent performance and accurate testing have been used in medical and safety detection. Food safety, environmental protection and other fields. Because biosensor has the advantages of simple detection process, high accuracy, high sensitivity and so on, it has become the research focus of various detection applications. However, with the continuous popularization of electrochemical biosensor, In recent years, new types of sensors, such as printed electrodes and enzyme membrane reactors, have emerged. In addition to the high efficiency and stability of detection, the sensor devices tend to be miniaturized and portable. In this paper, the flexible glucose and lactic acid sensors and the enzyme membrane sensors with excellent performance are prepared to achieve the high efficiency, portability and miniaturization of the sensors. Conductive polymer films were prepared by adding PEDOT:PSS aqueous solution and MWNT-OH into SBS thin films and used as a sensor electrode to immobilize oxidase by cross-linking chitosan and glutaraldehyde. A flexible glucose biosensor and a flexible lactate biosensor with good electrochemical performance were prepared. The linear range of detection is 0 ~ 6 mm, and the linear regression equation is I ~ (2.21) C ~ (1.4455A) R ~ (2 +) (0.996). The detection sensitivity is 2.21 渭 A / m ~ (m) and the detection limit is 12 渭 m ~ (-1) S / N ~ (3). A flexible lactic acid biosensor is prepared. The linear range of the two sensors is 0.2mM, the linear regression equation is 0.5796C, the sensitivity is 7.00 渭 A / m and the detection limit is 7.87 渭 M / N ~ (3 +). The two kinds of sensors have good stability and anti-interference performance. Secondly, graphene oxide has been successfully prepared by the improved Hummers method, and the detection limit is 7.87 渭 m 路m ~ (-1) 路m ~ (-1), and the detection limit is 7.87 渭 m ~ (-1) 路m ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). G-Pt NPswas prepared by solvothermal method and lactic acid biosensor was prepared as an electrochemical sensitive material. It has good stability and anti-interference performance. The linear range of detection is 0 ~ 1.2 mm, and the linear regression equation is I _ (2.08) ~ (0.1623) N ~ (2 +) O ~ (2 +), the sensitivity is 2.08 渭 A / m ~ (m), the detection limit is 12.5 渭 m ~ (-1) S / N ~ (3 +). At the same time, the prepared flexible lactate biosensor has good detection stability and reproducibility. The sensitivity of detection is 2.08 渭 A / m M. by real-time detection of human sweat, it is found that the flexible lactate sensor can detect the sweat of human body. Finally, the substrate of conductive film is prepared by adding MWNT-OH to the PVB film, and the oxidase is modified. Glucose and lactic acid biosensors with excellent performance were prepared. The linear range of glucose biosensor prepared by 1PVB was 0 ~ 3 mm, and the linear regression equation was I _ (0.03) C _ (0.0305) ~ R ~ (2 +) 0.993.The detection sensitivity was 0.03 渭 am 路m ~ (-1) and the detection limit was 49.1 渭 m 路s ~ (-1) N ~ (3 +). After adding TTF, the linear regression equation was obtained. The linear range of detection was increased to 0 ~ 32 mm, and the linear regression equation was I _ (0.26) C _ (0.4899) R ~ (2 +) 0.90. The detection sensitivity was 0.26 渭 A / m 路m ~ (-1) and the detection limit was 62.8 渭 m ~ (-1) m ~ (-1) 路m ~ (-1). The linear detection concentration range was greatly increased. In addition, the lactic acid sensor was prepared by using 1PVB. The linear range of detection is 0.5 ~ (1.5) mm, the linear regression equation is I _ (0.20) C 0.0967H ~ (2 +) ~ (2 +) 0.992.The detection sensitivity is 0.20 渭 A / m 路m ~ (-1) and the detection limit is 18.8 渭 m ~ (-1) m ~ (-1). After adding TTF, a lactic acid sensor is prepared with 0.1 GA fixed LOx. The linear range of detection was 2 渭 m / m, the linear regression equation was 1.4956 渭 m / m, the sensitivity was 1.51 渭 A / m / m and the detection limit was 2.98 渭 M / N ~ (-1) N ~ (3 +). The linear detection concentration range was improved.
【學位授予單位】：深圳大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TB383.1;TP212

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