本文選題：溶解氧傳感膜　切入點(diǎn)：熒光猝滅原理　出處：《浙江工業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：溶解氧濃度檢測(cè)對(duì)于環(huán)境監(jiān)測(cè)、工業(yè)生產(chǎn)、臨床醫(yī)學(xué)以及水產(chǎn)養(yǎng)殖等領(lǐng)域均有十分重要的意義。傳統(tǒng)的溶解氧檢測(cè)方法是Clark電極法。然而,Clark電極容易被樣品中的其它組分污染(如H2S、蛋白質(zhì)等),并且在檢測(cè)過程中消耗氧氣,破壞檢測(cè)樣本。因此,近年來基于熒光猝滅原理的溶解氧傳感器以其靈敏度高、檢測(cè)速度快并能在線檢測(cè)等優(yōu)勢(shì)受到越來越多研究者的關(guān)注。作為光學(xué)溶解氧傳感器的核心組件,溶解氧傳感膜的性能尤為重要,它通常由氧敏熒光指示劑和支撐基質(zhì)組成。目前,隨著工業(yè)的發(fā)展,溶解氧傳感膜在長期穩(wěn)定性、靈敏度等方面仍需進(jìn)一步的提升。本文通過氟化有機(jī)改性溶膠-凝膠法制備高性能的基于熒光猝滅原理的溶解氧傳感膜,并采用可逆加成/斷裂鏈轉(zhuǎn)移(RAFT)細(xì)乳液界面聚合法制備包埋熒光指示劑的納米聚合物粒子,以探索提高溶解氧傳感膜性能的新方法。以四乙氧基硅烷(TEOS)和3,3,3-三氟丙基三甲氧基硅烷(TFP-TMOS)為前驅(qū)體,以三(4,7-聯(lián)苯-1,10-鄰菲Up啉)二氯化釕為氧敏熒光指示劑,采用氟化有機(jī)改性溶膠-凝膠法制備溶解氧傳感膜。通過單因素實(shí)驗(yàn),對(duì)溶解氧傳感膜的性能進(jìn)行了優(yōu)化,并且對(duì)優(yōu)化后傳感膜的表面形態(tài)進(jìn)行分析,同時(shí)結(jié)合溶解氧傳感器對(duì)傳感膜在靈敏度、響應(yīng)速度以及長期穩(wěn)定性等性能進(jìn)行檢測(cè)。結(jié)果表明,氟化有機(jī)改性溶膠-凝膠法制備的溶解氧傳感膜具有靈敏度高、響應(yīng)速度快、長期穩(wěn)定性好等優(yōu)點(diǎn)。以甲基丙烯酸甲酯(MMA)為反應(yīng)單體,石蠟和三(4,7-聯(lián)苯-1,10-鄰菲Up啉)二氯化釕的混合液為核心材料,自制雙親性大分子RAFT試劑充當(dāng)乳化劑和RAFT試劑,以偶氮異丁腈(AIBN)為引發(fā)劑,采用RAFT(可逆加成/斷裂鏈轉(zhuǎn)移)細(xì)乳液界面聚合法成功制備了包含熒光指示劑的納米聚合物粒子。主要研究了AIBN的用量對(duì)聚合反應(yīng)的轉(zhuǎn)化率和粒子形態(tài)的影響以及RAFT試劑對(duì)粒子粒徑的調(diào)節(jié)規(guī)律。結(jié)果表明:熒光指示劑被成功包裹到納米聚合物粒子內(nèi)部,聚合物粒子呈規(guī)整的球形結(jié)構(gòu),且粒徑分布均勻;粒子的粒徑隨RAFT試劑用量增大而減小。此外,進(jìn)一步探索了將所制備的包含熒光指示劑的納米聚合物粒子分散到優(yōu)化后的氟化有機(jī)改性溶膠-凝膠基質(zhì)中以制備溶解氧傳感膜。
[Abstract]:Determination of dissolved oxygen concentration for environmental monitoring, industrial production, Clinical medicine and aquaculture are of great significance. The traditional method for detection of dissolved oxygen is the Clark electrode. However, the Clark electrode is liable to be contaminated by other components of the sample (such as H2S, protein, etc.). Consuming oxygen in the process, Therefore, in recent years, the dissolved oxygen sensor based on the principle of fluorescence quenching has high sensitivity. More and more researchers pay attention to the advantages of high detection speed and on-line detection. As the core component of optical dissolved oxygen sensor, the performance of dissolved oxygen sensing film is particularly important. It usually consists of oxygen sensitive fluorescent indicators and supporting substrates. At present, with the development of industry, the dissolved oxygen sensing membrane is stable in the long run. In this paper, the fluorinated organic modified sol-gel method is used to prepare the high performance dissolved oxygen sensing film based on the principle of fluorescence quenching. The nano polymer particles embedded with fluorescent indicator were prepared by reversible addition / chain break transfer (RAFT) fine emulsion interfacial polymerization. In order to explore a new method to improve the performance of dissolved oxygen sensing film, using tetraethoxy silane (TEOS) and 3H 3fluoropropyl trimethoxy silane (TFP-TMOS) as the precursors, the oxygen sensitive fluorescence indicator of ruthenium dichloride (Ru _ 2O _ 2) was used as the indicator of oxygen-sensitive fluorescence, using trisodioxy-7- biphenyl -10- (o-phenanthroline) as fluorescent indicator, and trifluoropropyltrimethoxysilane (TFP-TMOS) as the precursor. The dissolved oxygen sensing membrane was prepared by fluorinated organic modified sol-gel method. The performance of the dissolved oxygen sensing film was optimized by single factor experiment, and the surface morphology of the optimized film was analyzed. At the same time, the sensitivity, response speed and long-term stability of the sensing film were tested by the dissolved oxygen sensor. The results show that the dissolved oxygen sensing film prepared by fluorinated organic sol-gel method has high sensitivity and fast response speed. Using methyl methacrylate (MMA) as reaction monomer, paraffin and Ru _ 2 as core materials, amphiphilic macromolecule RAFT reagent was used as emulsifier and RAFT reagent. Using azo-iso-butyronitrile (AIBN) as initiator, Nanosized polymer particles containing fluorescent indicators were successfully prepared by RAFT (reversible addition / chain break transfer) fine emulsion interfacial polymerization. The effects of the amount of AIBN on the conversion and particle morphology of the polymerization were studied. The results showed that the fluorescent indicator was successfully encapsulated into the inner of nano-polymer particles. The polymer particles have a regular spherical structure, and the particle size distribution is uniform, and the particle size decreases with the increase of the amount of RAFT reagent. In order to prepare the dissolved oxygen sensing film, the nanoparticles containing fluorescent indicator were dispersed into the fluorinated organic modified sol-gel matrix.
【學(xué)位授予單位】：浙江工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O631;TB383.2

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