本文選題：比色傳感器　切入點(diǎn)：葡萄糖檢測(cè)　出處：《中國(guó)科學(xué)技術(shù)大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：現(xiàn)階段,DNA技術(shù)已經(jīng)擴(kuò)展應(yīng)用到各個(gè)領(lǐng)域,以DNA為手段自組裝合成的納米智能材料在化學(xué)生物領(lǐng)域有著廣泛的應(yīng)用,尤其是作為比色計(jì)傳感在生物檢測(cè)方面的應(yīng)用十分引人注目。另外關(guān)于單個(gè)貴金屬納米粒子的暗場(chǎng)散射直以來(lái)都是科學(xué)家們感興趣并努力探索的方向。利用該技術(shù),可以為我們觀(guān)測(cè)化學(xué)反應(yīng),實(shí)時(shí)光學(xué)傳感,生物成像方面提供了一個(gè)更直觀(guān)的良好平臺(tái)。未來(lái),我們希望單個(gè)粒子的散射可以應(yīng)用到體內(nèi)或體外實(shí)時(shí)監(jiān)測(cè)等方面。而在明場(chǎng)模式下,觀(guān)測(cè)微納馬達(dá)(納米器械借助外界驅(qū)動(dòng)力運(yùn)動(dòng))的運(yùn)動(dòng)行為對(duì)我們高效、有效控制運(yùn)動(dòng)軌跡有著實(shí)驗(yàn)基礎(chǔ)意義。未來(lái),微納馬達(dá)將在分子檢測(cè)、蛋白質(zhì)運(yùn)載、核酸識(shí)別、癌細(xì)胞的捕捉、藥物的定向運(yùn)載和釋放、有害環(huán)境污染處理等方面展示它十分重要的功能意義。本論文的相關(guān)工作就是圍繞上述幾點(diǎn)來(lái)進(jìn)行的：1.近來(lái),關(guān)于建構(gòu)新型納米材料用于檢測(cè)葡萄糖濃度又有了新的突破,即基于合成穩(wěn)定納米銀顆粒的比色方法。銀納米粒子較金納米粒子和其它金屬粒子有更高的摩爾吸光系數(shù),可以呈現(xiàn)出褐色到黃色的明顯的肉眼可觀(guān)顏色。該類(lèi)比色計(jì)的檢測(cè)原理就是依賴(lài)于葡萄糖和葡萄糖氧化酶催化作用產(chǎn)生的過(guò)氧化氫來(lái)氧化刻蝕銀,從而使得黃色慢慢退去來(lái)實(shí)現(xiàn)比色檢測(cè)葡萄糖或過(guò)氧化氫的。該方法中用到的銀納米粒子較之前的模擬天然過(guò)氧化物的納米材料有很好的光學(xué)性質(zhì),相對(duì)高的穩(wěn)定性且具有更高的經(jīng)濟(jì)效益。而關(guān)于如何合成穩(wěn)定性好、分散性良好的銀納米材料一直是一個(gè)需要克服的難題。盡管科學(xué)家們?cè)诤铣摄y納米粒子工作上做了很多嘗試,但是仍存在不同程度的問(wèn)題,包括粒子的高度分散性,尺寸可控,光譜可調(diào)節(jié)等性質(zhì)。幸運(yùn)的是,DNA功能化技術(shù)和種子輔助沉積技術(shù)為我們制備具有相同光學(xué)性質(zhì)的銀納米材料提供了另一種思路。在本課題組之前的工作中,成功合成出了DNA包裹的金銀核殼納米結(jié)構(gòu)(Au@DNA@AgNPs)。由于金核的存在可以更方便我們制備出尺寸可控,粒徑均一的納米結(jié)構(gòu)。另外,由于Au核上修飾的大量DNA分子,使得合成的納米粒子表面帶上了大量負(fù)電荷從而增強(qiáng)了粒子間的庫(kù)倫排斥作用,更加利于穩(wěn)定納米粒子。利用該結(jié)構(gòu)我們成功構(gòu)筑了用于檢測(cè)葡萄糖的新型比色傳感器,并實(shí)現(xiàn)了胎牛血清中葡萄糖的檢測(cè)。2.光學(xué)顯微鏡技術(shù)的不斷進(jìn)步為我們觀(guān)測(cè)粒子行為提供了一個(gè)更加廣闊的舞臺(tái)。金納米粒子作為貴金屬納米材料的代表,具有強(qiáng)吸收和強(qiáng)散射的光學(xué)特性。一般情況下,粒徑越大的顆粒,光散射越強(qiáng),將光轉(zhuǎn)化成熱或其他能量的概率越小。關(guān)于單個(gè)粒子的暗場(chǎng)散射觀(guān)測(cè)一直是科研人員關(guān)注卻涉足較少的一個(gè)領(lǐng)域。所以在這方面的探索是一項(xiàng)很有意義的工作。這里我們選擇合成直徑大于40 nm的金納米粒子并用于觀(guān)測(cè)暗場(chǎng)(dark field microspectroscopy, DFM)中的光散射情況,并拓展到金納米粒子修飾互補(bǔ)DNA鏈后雜交行為的初步探索。另一方面,由于納米技術(shù)日益受到人們的關(guān)注,尤其是關(guān)注的熱點(diǎn)之一以微納馬達(dá)為代表的微型驅(qū)動(dòng)機(jī)器。微納馬達(dá)發(fā)展至今,已經(jīng)取得了很多重大的突破,但是在效率、功能、壽命以及環(huán)境應(yīng)用方面還有很多不足。而適用于大量制造的微納馬達(dá)制備方法尚處在研究階段。關(guān)于其如何更好地應(yīng)用環(huán)境中的能量,實(shí)現(xiàn)微納馬達(dá)的自驅(qū)動(dòng),以及有效控制運(yùn)動(dòng)方向上也處于實(shí)驗(yàn)階段。在我們實(shí)驗(yàn)階段我們參考文獻(xiàn)選擇MnO2和過(guò)氧化氫體系,在明場(chǎng)模式下(bright filed microspectroscopy, BFM)進(jìn)行氣體推進(jìn)型微納馬達(dá)運(yùn)動(dòng)行為的初步觀(guān)測(cè)。
[Abstract]:At present, DNA technology has been extended to various fields, taking DNA as a means of self assembled nano intelligent synthetic materials in chemical and biological fields have a wide range of applications, especially applications as colorimetric sensing in biological detection is very compelling. In addition a single dark field light scattering of noble metal nanoparticles have long been the scientists interest and efforts to explore the direction. Using this technology, we can observe the chemical reaction, real-time optical sensing, provides a good platform for more intuitive biological imaging. In the future, we hope that the scattering of individual particles can be applied to the aspects of in vivo or in vitro real-time monitoring and so on. And in the bright field mode, micro observation nano motor (nano devices with the external driving force of the movement movement) behavior to our efficient, effective control of motion trajectory is the experimental basis. The future will be reached in the micro Nama Sub carrier protein, nucleic acid detection, identification, cell capture, drug delivery and directional release, demonstrating its functional significance of an important aspect of harmful environmental pollution treatment. The related work of this paper is focused on the above points to the 1.: Recently, on the construction of new nano materials for the detection of glucose concentration and the the new breakthrough, which is based on the synthesis of stable silver nanoparticles colorimetric method. The silver nanoparticles have higher molar absorptivity than gold nanoparticles and other metal particles can exhibit obvious yellow to brown the meat color. The detection principle of the considerable eye color meter analogy is dependent on the catalytic effect of glucose and glucose oxidase the hydrogen peroxide oxidation etching silver, so that the Yellow slowly receded to realize the colorimetric detection of glucose or hydrogen peroxide. The method used in the silver nano particle Compared to the previous sub nanometer material to simulate natural peroxide optical properties good stability, relatively high and has higher economic benefit. On how to synthesis of good stability, good dispersion of silver nano materials have been a need to overcome the problem. Although scientists have made many attempts in the synthesis of silver nanoparticles work however, there are still various problems, including high dispersion, particle size controllable, adjustable spectral properties. Fortunately, the function of DNA technology and seed assisted deposition technology for our preparation of silver nano materials with optical properties provides another way. Before this research group in the work, successfully synthesized gold and silver core-shell structure DNA package (Au@DNA@AgNPs). Because the nuclear presence can be more convenient for the preparation of size controlled nano structure, uniform particle size on the other. Outside, due to the large number of DNA Au nuclear molecular modification, the surface of nanoparticles synthesis with a large amount of negative charge so as to enhance the repulsion between particles in Kulun, more conducive to the stability of nanoparticles. Using this structure we build a successful model for the detection of glucose colorimetric sensor, and realizes the continuous progress of glucose in fetal bovine serum the detection of.2. optical microscope technology provides a wide stage for our observations of particle behavior. Gold nanoparticles as a representative of the noble metal nanometer material, optical characteristics of strong absorption and high scattering. In general, the larger particle size, light scattering is strong, light probability into heat or other energy is small. Dark field light scattering has been observed on single particle researchers focus on an area is less involved. So the research in this aspect is a very interested The meaning of work. Here we choose the synthesis of more than 40 nm in diameter gold nanoparticles and to observe the dark field (dark field microspectroscopy, DFM) light scattering of the gold nanoparticles modified and extended to explore complementary hybridization behavior of DNA chain. On the other hand, because of nanotechnology has attracted more and more attention, especially in micro one of the research hotspots to drive the machine. As the representative of the micro motor micro motor development so far, has made many major breakthroughs, but the efficiency function, there are many shortcomings in life and environmental applications. And is suitable for mass production of micro motor preparation methods are still in research on how to better the. The application environment of the energy, realize self driven micro motor, and effectively control the direction of movement is still at the experimental stage. In our experimental stage we reference M The nO2 and the hydrogen peroxide system, in the Ming field model (bright filed microspectroscopy, BFM), carry out the preliminary observation of the motion behavior of the gas propelled micromotor.

【學(xué)位授予單位】：中國(guó)科學(xué)技術(shù)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：TB383.1;TP212

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 賈廷見(jiàn);;銀膠的非線(xiàn)性光學(xué)特性研究[J];商丘職業(yè)技術(shù)學(xué)院學(xué)報(bào);2015年05期

相關(guān)碩士學(xué)位論文 前1條

1 劉飛;金屬納米顆粒的結(jié)構(gòu)及其介質(zhì)復(fù)合材料的非線(xiàn)性光學(xué)性質(zhì)研究[D];武漢科技大學(xué);2014年

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本文編號(hào)：1557624