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  本文關鍵詞： 局域表面等離子體共振 三角形銀納米粒子 三角納米環(huán)結構 雙氧水的測定 葡萄糖的測定　出處：《蘇州大學》2015年碩士論文　論文類型：學位論文

【摘要】：貴金屬納米材料如金、銀等有較為獨特的局域表面等離子體共振(LSPR)特性,它們大部分可以應用于催化、生物傳感以及醫(yī)藥等領域。與球形納米粒子相比,三角形銀納米粒子的LSPR吸收峰對周圍介質折射率變化極其敏感。糖尿病是一個世界性的公共衛(wèi)生問題,已被認為是導致死亡的全球性疾病之一。糖尿病的診斷和控制需要密切地監(jiān)測血液中葡萄糖的濃度。傳統(tǒng)的光度分析法是用葡萄糖氧化酶或辣根過氧化酶等,且采用有機顯色劑來測定。本論文合成了金銀三角納米環(huán)結構,并應用于比色法測定葡萄糖,金銀三角納米環(huán)首先可以代替葡萄糖氧化酶催化氧化葡萄糖,接著利用生成的過氧化氫刻蝕環(huán)中的銀原子使其結構發(fā)生變化,從而引起金銀三角環(huán)LSPR吸收峰的變化。由此可便捷地用該方法檢測葡萄糖,避免使用任何酶及顯色劑。論文第二章是采用晶種生長的方法制備三角形銀納米粒子。首先是用循環(huán)伏安法在ITO玻璃表面直接電沉積銀納米種子,然后將制備好的銀納米晶種放在生長液中生長,形成三角形結構。利用紫外-可見吸收分光光度法、掃面電鏡等方法對其表征。三角形結構的銀納米粒子紫外吸收峰在650nm左右。同時討論了生長液中各試劑濃度、生長時間及溫度對形成三角形結構的影響。論文第三章是在溶液中合成金銀三角納米環(huán)結構,并將其應用于LSPR生物傳感。根據文獻報道采用雙氧水在溶液中合成三角形銀納米粒子,在上述合成的溶液中加入還原劑抗壞血酸等生成金銀三角納米環(huán),并對其進行表征;同時考察了不同試劑加入量和反應時間對反應的影響。將金銀三角納米環(huán)結構應用于測定雙氧水及葡萄糖濃度,得出三角形環(huán)納米結構測定雙氧水和葡萄糖時分別在一定的濃度范圍內具有良好的線性。測定葡萄糖時其線性范圍500ppb~5ppb,檢測下限(LCD)達4uM,相關系數(shù)為0.99884。以人體血液為實際樣品測定,回收率達93.4%-102%。由此證實了金銀三角形環(huán)納米結構傳感器具有無試劑且方便的優(yōu)點,因此制備這類傳感器具有十分重要的意義。
[Abstract]:Noble metal nanomaterials such as gold and silver have unique local surface plasmon resonance (LSPRs) properties, which can be used in catalysis, biosensor, medicine and other fields. The LSPR absorption peak of triangular silver nanoparticles is extremely sensitive to the refractive index changes in the surrounding media. Diabetes is a worldwide public health problem. Diabetes diagnosis and control require close monitoring of blood glucose concentrations. Traditional photometric analysis uses glucose oxidase or horseradish peroxidase, etc. In this paper, the structure of gold and silver triangulated rings was synthesized and applied to the determination of glucose by colorimetric method. The gold and silver triangular rings can be used to catalyze the oxidation of glucose instead of glucose oxidase. Then the structure of the silver atom in the ring was changed by hydrogen peroxide etching, which caused the change of the LSPR absorption peak of the gold and silver triangulation ring. This method can be used to detect glucose conveniently. In chapter 2, triangular silver nanoparticles were prepared by seed growth. First, silver nanoparticles were electrodeposited on the surface of ITO glass by cyclic voltammetry. Then the prepared silver nanocrystalline seeds were grown in the growth solution to form a triangular structure. UV-Vis absorption spectrophotometry was used. The UV absorption peak of triangular silver nanoparticles was about 650 nm. The concentration of reagents in the growth solution was also discussed. The influence of growth time and temperature on the formation of triangular structure. According to the literature, triangular silver nanoparticles were synthesized by hydrogen peroxide in the solution, and the gold and silver triangulated rings were formed by adding ascorbic acid into the above synthetic solution, and the gold and silver triangular rings were characterized. At the same time, the effects of different reagents and reaction time on the reaction were investigated. The gold and silver triangular nanospheres were used to determine the concentration of hydrogen peroxide and glucose. The results showed that the linear range of hydrogen peroxide and glucose was in a certain concentration range. The linear range of glucose was 500 ppb ~ 5 ppb, the detection limit was 4 渭 M, and the correlation coefficient was 0.99884.The results showed that the linear range was 500 ppb ~ (5) ppb, and the correlation coefficient was 0.9988 4. The recovery rate is 93.4-102. It is proved that the gold and silver triangular ring nanosensors have the advantages of no reagent and convenience, so the preparation of this kind of sensors is of great significance.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：O614.12;TB383.1

【共引文獻】

相關期刊論文 前1條

1 焦成鵬;黃自力;張海軍;張少偉;;置換反應制備雙金屬納米催化劑[J];化學進展;2015年05期

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本文編號：1542128