本文關鍵詞： SEBS 糖基功能單體 表面圖案化 血液相容性 水凝膠　出處：《煙臺大學》2017年碩士論文　論文類型：學位論文

【摘要】：以D-(+)-葡萄糖酸-1,5-內(nèi)酯和甲基丙烯酸-2-氨基乙基酯鹽酸鹽為原料,制備了含有不飽和雙鍵的糖基功能單體-甲基丙烯酸-2-(N-葡萄糖酰胺)乙酯(GAMA),利用傅立葉紅外光譜(FTIR)和氫核磁共振譜(1H NMR)對單體結構進行了表征。采用紫外引發(fā)表面接枝技術將GAMA接枝到聚(苯乙烯-b-(乙烯-co-丁二烯)-b-苯乙烯)(SEBS)膜表面,形成分子刷,對材料表面進行生物相容性改性,功能化的SEBS膜結構通過衰減全反射紅外光譜(ATR-FTIR)和X-光電子能譜(XPS)進行分析,通過靜態(tài)去離子水接觸角、蛋白質(zhì)吸附、血小板粘附以及溶血等實驗,考察了改性后SEBS表面的親水性和血液相容性。結果表明,與SEBS原膜相比,PGAMA分子刷改性的SEBS膜表面具有良好的親水性、抗蛋白吸附、抗血小板粘附性能,并且有效的降低了溶血的發(fā)生。通過紫外引發(fā)表面接枝在SEBS表面接枝2-丙烯酰胺-2-甲基丙磺酸(AMPS)分子刷得到良好的親水性表面,用于抵抗蛋白質(zhì)吸附和血細胞粘附;然后,結合光掩模板在PAMPS分子刷表面制備圖案化的PGAMA分子刷區(qū)域,從而構建了二元圖案化的親水性分子刷結構。隨后利用糖與凝集素之間的作用,得到了尺寸可控、形貌均勻清晰的植物血球凝集素(PHA)陣列。利用PHA對紅細胞的特異性識別作用,通過血細胞粘附實驗發(fā)現(xiàn),PHA陣列幾乎不粘附血小板而能夠高效捕捉紅細胞(RBCs),并且捕獲的紅細胞基本保持其原有的完整性和功能。因此,實現(xiàn)了紅細胞在材料表面的無損害捕獲。通過紫外引發(fā)表面接枝技術方法在SEBS膜表面接枝第一層聚乙二醇(PEG)分子刷;然后采用紫外引發(fā)接枝聚合與光刻蝕技術相結合的方法,在第一層分子刷的基礎上構建了結構層次清晰均勻,圖案化區(qū)域邊界明顯的二元三維圖案化水凝膠結構。利用ATR-FTIR和XPS對功能化的SEBS各層進行表面結構分析;利用偏光顯微鏡(POM)和掃描電子顯微鏡(SEM)等手段觀察該體系的表面形貌;通過靜態(tài)去離子水接觸角、蛋白質(zhì)吸附、血小板粘附以及紅細胞粘附等實驗,考察了該體系表面的親水性和血液相容性。其中,第一層親水層PEG分子刷,可以有效抵抗蛋白質(zhì)和血細胞的粘附,第二層圖案化水凝膠,由于表面親水性和表面獨特的拓撲結構的共同影響,既不吸附蛋白質(zhì)也不粘附血細胞,因此,該體系能夠很好的抵抗蛋白質(zhì)的非特異性吸附和血細胞的粘附,進而改善了SEBS的血液相容性。
[Abstract]:D-Gluconoic acid-5-lactone and methacrylate -2-aminoethyl ester hydrochloride were used as raw materials, The glycosyl functional monomers of glycosyl groups with unsaturated double bonds were prepared. The structure of the monomers was characterized by Fourier transform infrared spectroscopy (FTIR) and hydrogen nuclear magnetic resonance spectroscopy (1H NMRs). GAMA was grafted onto the surface of poly (styrene-b-(ethylene-co- butadiene)-b-styrene) film by surface grafting. Molecular brushes were formed to modify the biocompatibility of the materials. The functionalized SEBS membrane structure was analyzed by attenuated total reflectance infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS), and the protein was adsorbed by static deionized water contact angle. The hydrophilicity and blood compatibility of modified SEBS surface were investigated by platelet adhesion and hemolysis experiments. The results showed that the surface of SEBS membrane modified by PGAMA molecular brush had good hydrophilicity and anti-protein adsorption. The anti-platelet adhesion property, and effectively reduced the occurrence of hemolysis. The surface of SEBS was grafted onto the surface of SEBS to obtain good hydrophilic surface. It is used to resist protein adsorption and blood cell adhesion; then, a patterned PGAMA molecular brush region is prepared on the surface of the PAMPS molecular brush by combining the photomask template. Thus, a binary patterned hydrophilic molecular brush structure was constructed. Then, by using the interaction between sugar and lectin, a controllable size and uniform morphology of plant hemagglutinin (PHA) array was obtained. The specific recognition effect of PHA on red blood cells was obtained. By blood cell adhesion test, it was found that PHA array could capture RBCsN efficiently and retain its original integrity and function. The red blood cells on the surface of the material were captured without damage. The first layer of polyethylene glycol (PEG) molecular brush was grafted on the surface of SEBS membrane by UV-induced surface grafting, and then the UV initiated graft polymerization was combined with photoetching. On the basis of the first layer molecular brush, a binary three-dimensional patterned hydrogel structure with clear and uniform structure and obvious boundary of patterned region was constructed. The surface structure of the functional SEBS layers was analyzed by ATR-FTIR and XPS. The surface morphology of the system was observed by means of polarizing microscope (POM) and scanning electron microscope (SEM). The surface morphology of the system was observed by static deionized water contact angle, protein adsorption, platelet adhesion and erythrocyte adhesion. The surface hydrophilicity and blood compatibility of the system were investigated. The first hydrophilic layer PEG molecular brush could effectively resist the adhesion of protein and blood cells, and the second layer was patterned hydrogel. Because of the influence of surface hydrophilicity and surface unique topological structure, neither protein nor blood cells are adsorbed. Therefore, the system can resist the non-specific adsorption of proteins and the adhesion of blood cells. Thus, the blood compatibility of SEBS was improved.
【學位授予單位】：煙臺大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TB306

【參考文獻】

中國期刊全文數(shù)據(jù)庫 前3條

1 陳寶林;王東安;;用于心血管醫(yī)療裝置的聚合物材料表面構建與生物相容性評價:聚合物生物材料表面的內(nèi)皮細胞組織工程化改性（英文）[J];中國組織工程研究;2016年30期

2 Yuan-qing Song;Yun-long Gao;Zhi-cheng Pan;Yi Zhang;李潔華;Kun-jie Wang;Jian-shu Li;譚鴻;Qiang Fu;;Preparation and Characterization of Controlled Heparin Release Waterborne Polyurethane Coating Systems[J];Chinese Journal of Polymer Science;2016年06期

3 邱炳崧;MAI Weizhong;曾戎;TU Mei;ZHAO Jianhao;ZHA Zhengang;;Microwave-assisted Immobilization of Heparin onto Polyurethane Surface for Improving Blood Compatibility[J];Journal of Wuhan University of Technology(Materials Science Edition);2014年05期

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本文編號：1518495