本文關(guān)鍵詞： 生物分子 銀納米簇 金屬離子 生物硫醇 熒光分析法　出處：《山西大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：銀納米簇(Ag NCs)作為一種新型的熒光團(tuán),具有制備成本低廉、化學(xué)性質(zhì)活潑、表面易修飾、水溶性良好等優(yōu)點(diǎn),作為性能優(yōu)良的熒光探針,引起了研究者廣泛的興趣。論文中,我們以不同種類的生物分子作為保護(hù)劑兼配體,合成了不同功能的發(fā)光Ag NCs,并基于熒光猝滅機(jī)制,建立了對(duì)金屬離子、生物硫醇的分析檢測(cè)方法。第一章：綜述了Ag NCs的合成、光學(xué)性質(zhì)及其應(yīng)用方面的研究進(jìn)展。第二章：設(shè)計(jì)了一種環(huán)狀部分富含胞嘧啶堿基C,二級(jí)結(jié)構(gòu)呈發(fā)卡狀的單鏈DNA分子。以該發(fā)卡狀DNA作為模板,合成了高量子產(chǎn)率的、發(fā)紅色熒光的DNA/Ag NCs,當(dāng)Hg2+存在時(shí),能有效地將其熒光猝滅,進(jìn)而發(fā)展了一種靈敏度高、選擇性好的檢測(cè)Hg2+的方法。該方法可在1.0×10-10mol/L-1.2×10-8mol/L的線性范圍內(nèi)實(shí)現(xiàn)對(duì)Hg2+的測(cè)定,并在S/N=3時(shí),檢出限為1.0×10-10mol/L。通過紫外可見吸收光譜的初步研究,熒光猝滅可能是由于5d10(Hg2+)-4d10(Ag+)之間的親金屬作用所致。第三章：以巰基小分子化合物——谷胱甘肽(GSH)為配體,采用超聲法一步合成了發(fā)藍(lán)色熒光的GSH@Ag NCs,并運(yùn)用透射電鏡(TEM)、紫外可見吸收光譜和熒光光譜對(duì)其進(jìn)行了表征。發(fā)現(xiàn)Fe3+存在時(shí),可使其熒光猝滅,建立了高靈敏測(cè)定Fe3+的方法,在5×10-7mol/L-2×10-5 mol/L的線性范圍內(nèi)對(duì)Fe3+有良好響應(yīng),S/N=3時(shí)檢出限為1.2×10-7 mol/L,將該方法成功用于人血清樣品中鐵含量的測(cè)定。當(dāng)引入絡(luò)合劑EDTA時(shí),Fe3+-GSH@Ag NCs體系的熒光恢復(fù),這既為Fe3+與GSH@Ag NCs的作用機(jī)制提供了一種新的理解,也使GSH@Ag NCs可能被開發(fā)為off/on新型可逆熒光探針。第四章：以牛血清白蛋白(BSA)做保護(hù)劑,合成了發(fā)紅色熒光的BSA-Ag NCs,其熒光能被小分子生物硫醇半胱氨酸(Cys)、同型半胱氨酸(Hcy)和谷胱甘肽(GSH)猝滅,這可能是由巰基和銀之間的親和作用所致。用BSA-Ag NCs作為納米熒光探針,建立了生物硫醇的新型分析檢測(cè)方法,測(cè)定Cys、Hey、GSH的線性范圍分別為2.0×10-6mol/L-9.0×10-5mol/L、2.0×10-6mol/L-1.2×10-4mol/L、1.0×10-5 mol/L-8.0×10-5mol/L;S/N=3時(shí),檢出限分別為8.1×10-7 mol/L、1.0×10-6 mol/L、1.1×10-6mol/L。此方法可成功地用于人血漿樣品中生物硫醇含量的測(cè)定,回收率94.83-105.24%,表明BSA-Ag NCs在化學(xué)和生物傳感方面具有潛在的應(yīng)用價(jià)值。第五章：對(duì)本論文中Ag NCs作為納米熒光探針,在金屬離子和生物硫醇分析檢測(cè)方面的研究結(jié)果做了總結(jié),并對(duì)下一步工作進(jìn)行了展望。
[Abstract]:Silver nanoclusters Ag NCs, as a new type of fluorescence, have the advantages of low preparation cost, active chemical properties, easy surface modification, good water solubility and so on, so they can be used as fluorescent probes with excellent properties. In this paper, different kinds of biomolecules were used as protective agents and ligands to synthesize Ag NCswith different functions and based on fluorescence quenching mechanism. A method for the analysis and detection of metal ions and biological mercaptan was established. Chapter 1: the synthesis of Ag NCs was reviewed. Progress in optical properties and applications. Chapter 2: a circular partially cytosine C- rich single-stranded DNA molecule with a hairpin structure was designed. The hairpin DNA was used as a template. DNA/Ag NCs with high quantum yield and red fluorescence have been synthesized. In the presence of Hg2, it can effectively quench its fluorescence, thus developing a kind of high sensitivity. The method can be used to determine Hg2 in the linear range of 1.0 脳 10 ~ (-10) mol / L ~ (-1) 脳 10 ~ (-8) mol / L ~ (-1) 脳 10 ~ (-8) mol 路L ~ (-1) 路L ~ (-1). At S / N = 3:00, the detection limit is 1.0 脳 10 ~ (-10) mol / L. Fluorescence quenching may be due to the metalphilic interaction between 5 d 10 Hg2 + -4 d 10 Ag. Chapter 3: glutathione glutathione glutathione (GSH) is used as ligand. GSH@Ag NCs with blue fluorescence were synthesized by ultrasonic method. UV-Vis absorption and fluorescence spectra were used to characterize it. It was found that Fe3 can quench its fluorescence in the presence of Fe3, and a highly sensitive method for the determination of Fe3 was established. In the linear range of 5 脳 10 ~ (-7) mol / L ~ (-2) 脳 10 ~ (-5) mol/L, the detection limit for Fe3 is 1.2 脳 10 ~ (-7) mol / L at 3:00. The method was successfully applied to the determination of iron in human serum samples. The fluorescence recovery of Fe _ 3-GSH _ H NCs system was obtained when the complex agent EDTA was introduced. This provides a new understanding of the mechanism of Fe3 and GSH@Ag NCs. GSH@Ag NCs may also be developed as a novel reversible fluorescence probe for off/on. Chapter 4th: bovine Serum Albumin (BSA) as Protective Agent. A red fluorescent BSA-Ag NCS was synthesized, which can be quenched by cysteine (cysteine) and glutathione (Glutathione). This may be due to the affinity between mercapto and silver. Using BSA-Ag NCs as a nano-fluorescent probe, a new analytical method for the detection of biological mercaptan was established. The linear range of GSH is 2.0 脳 10-6 mol / L 9.0 脳 10 -5 mol / L, 2.0 脳 10 -6 mol / L -1. 2 脳 10 -4 mol / L. 1.0 脳 10-5 mol / L 8.0 脳 10-5 mol / L; S / N = 3:00, the detection limit was 8.1 脳 10 ~ (-7) mol / L ~ (-1) 脳 10 ~ (-6) mol/L. 1.1 脳 10 ~ (-6) mol 路L ~ (-1) 路L ~ (-1). The method has been successfully applied to the determination of biological mercaptan in human plasma samples with recoveries of 94.83-105.24%. It shows that BSA-Ag NCs has potential application value in chemical and biological sensing. Chapter 5th: using Ag NCs as a nano-fluorescence probe in this thesis. The results of analysis and detection of metal ions and biological mercaptan were summarized, and the future work was prospected.
【學(xué)位授予單位】：山西大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O657.3;TB383.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉琳;湯響林;吳加勝;劉衛(wèi)敏;;基于分子內(nèi)電荷轉(zhuǎn)移機(jī)制的巰基熒光比色化學(xué)傳感器[J];影像科學(xué)與光化學(xué);2014年03期

相關(guān)博士學(xué)位論文 前2條

1 穆親;量子點(diǎn)熒光探針的設(shè)計(jì)及檢測(cè)應(yīng)用[D];華東理工大學(xué);2014年

2 劉大穎;金屬離子熒光傳感器的設(shè)計(jì)、合成及在環(huán)境和生化領(lǐng)域的應(yīng)用[D];南開大學(xué);2014年

相關(guān)碩士學(xué)位論文 前8條

1 張革;電合成稠環(huán)芳香聚合物在水/醇溶液中的熒光傳感[D];江西科技師范大學(xué);2013年

2 呂佳;羅丹明衍生物的合成及性質(zhì)研究[D];延邊大學(xué);2013年

3 劉衛(wèi);核酸探針電化學(xué)傳感器用于小分子和肌紅蛋白檢測(cè)的研究[D];湖南大學(xué);2013年

4 顧楨燕;幾種稠環(huán)化合物新型熒光探針及其性能研究[D];南京理工大學(xué);2014年

5 王軍;化學(xué)修飾微型電極—毛細(xì)管電泳法測(cè)定生物活性物質(zhì)[D];山東師范大學(xué);2014年

6 侍金敏;巰基有機(jī)熒光小分子和無機(jī)—有機(jī)雜化熒光傳感器的合成及性質(zhì)研究[D];蘭州大學(xué);2014年

7 陳琪雯;新型碳基納米材料電極的研制及其在食品藥品分析中的應(yīng)用研究[D];復(fù)旦大學(xué);2013年

8 張志美;生物材料—熒光貴金屬納米簇的合成與性能分析[D];湖南大學(xué);2014年

，

本文編號(hào)：1479897