本文關(guān)鍵詞： 硫酸鈣 摻鍶β-磷酸三鈣 復(fù)合小球 堆垛成型 多孔支架　出處：《昆明理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：生物陶瓷材料由于具有良好的生物相容性、生物降解性、骨傳導(dǎo)性和骨誘導(dǎo)性等,常被用于修復(fù)骨組織缺損、替代失去功能或衰竭的組織、器官的部分或全部。而硫酸鈣、磷酸鈣類材料是目前應(yīng)用最為廣泛的骨修復(fù)用生物陶瓷材料。本文所研究的硫酸鈣/摻鍶β-TCP復(fù)合材料比單一材料在生物活性、降解性等方面具有更多的優(yōu)勢,具有更廣泛的應(yīng)用前景。本文采用攪拌噴霧干燥法成功制備出硫酸鈣/摻鍶β-TCP復(fù)合小球;為了提高復(fù)合材料的性能,隨后,分別用二氧化硅、明膠、羧甲基纖維素鈉與制備的復(fù)合小球進(jìn)行復(fù)合,得到了(SiO_2、明膠和羧甲基纖維素鈉)/硫酸鈣/摻鍶β-TCP復(fù)合多孔生物支架,并對其進(jìn)行成分、結(jié)構(gòu)和性能等方面的分析。本文進(jìn)行了以下研究:以碳酸鈣(CaCO_3、硝酸鍶(Sr(NO_3)_2)、磷酸(H3PO_4)為原料,用沉淀法制備了摻鍶β-磷酸三鈣粉體。該粉體中β-TCP為主相占86.7%;該粉體的粒徑大小主要分布在10μm～100μm范圍內(nèi),球磨后粉體粒徑為0.5μm～10μm,摻鍶量對粉體粒度大小沒有較大的影響;鍶摻入β-TCP后二者形成了置換固溶體,但摻鍶量對晶體結(jié)構(gòu)影響不大。由粉體細(xì)胞毒性實(shí)驗(yàn)結(jié)果可知,該粉體無細(xì)胞毒性。采用攪拌噴霧干燥法制備硫酸鈣/摻鍶β-TCP的復(fù)合小球。通過正交試驗(yàn)設(shè)計(jì)篩選出制備復(fù)合小球的最佳工藝方案,即:當(dāng)CaSO_4粉末添加量、粘接劑的濃度、摻鍶β-TCP濃度和摻鍶β-TCP所占CaSO_4粉末添加量的比例分別為2.5g,10 wt%,10 wt%和30wt%時(shí),可制備出造球率達(dá)75%左右,粒徑在0.95-1.35mm之間的復(fù)合小球顆粒。細(xì)胞毒性結(jié)果表明,該顆粒細(xì)胞毒性為0級或1級,即無細(xì)胞毒性。采用硅膠、明膠和羧甲基纖維素鈉三種物質(zhì),通過堆垛聚集的方法制備出多孔(SiO_2、明膠和羧甲基纖維素鈉)/復(fù)合小球生物支架。研究結(jié)果表明:三種復(fù)合支架的孔隙率均分布在60%左右;三種復(fù)合支架都具有不規(guī)則的孔洞結(jié)構(gòu),小球與小球之間形成的孔隙尺寸在0.2-1.Omm之間,SiO_2/復(fù)合小球生物支架孔徑主要分布在100-300μm;明膠/復(fù)合小球生物支架孔徑主要分布在100-500μm;羧甲基纖維素鈉/復(fù)合小球生物支架孔徑主要分布在50-300μm。SiO_2/復(fù)合小球生物支架的平均抗壓強(qiáng)度約為0.1Mpa,明膠/復(fù)合小球生物支架的平均抗壓強(qiáng)度達(dá)到6.3Mpa,羧甲基纖維素鈉/復(fù)合小球生物支架的平均抗壓強(qiáng)度為0.7Mpa;體外生物降解實(shí)驗(yàn)發(fā)現(xiàn),三種支架浸泡于SBF的過程中均有磷灰石的沉積,說明其具有良好的生物活性。其中,明膠/復(fù)合小球生物支架的降解周期最長,在本實(shí)驗(yàn)的80天周期內(nèi)仍未完全降解,羧甲基纖維素鈉/復(fù)合小球生物支架的降解周期較短,約為40天,SiO_2/復(fù)合小球生物支架在本實(shí)驗(yàn)的80天左右基本降解完全。三種復(fù)合支架細(xì)胞毒性為0級或1級,即無細(xì)胞毒性。
[Abstract]:Because of its good biocompatibility, biodegradability, bone conductivity and bone inductivity, bioceramic materials are often used to repair bone defects and replace the tissues with loss of function or failure. Part or all of the organ. And calcium sulfate. Calcium phosphate is the most widely used bioceramic material for bone repair at present. The calcium sulfate / strontium doped 尾 -TCP composite is more bioactive than a single material in this paper. In this paper, calcium sulfate / strontium doped 尾 -TCP composite pellets were successfully prepared by agitation spray drying method. In order to improve the properties of the composites, SiO2 was prepared by using silica, gelatin and sodium carboxymethyl cellulose respectively. Gelatin and carboxymethyl cellulose sodium / calcium sulfate / strontium 尾 -TCP composite porous scaffolds, and their composition. Analysis of structure and properties. The following studies have been carried out in this paper: calcium carbonate Caco _ 3s _ 3, strontium nitrate Srno _ 3s _ 3s _ 2s _ 2, phosphate _ (H _ 3PO _ 4) as raw materials. Sr-doped 尾 -tricalcium phosphate powder was prepared by precipitation method. The particle size of the powder is mainly distributed in the range of 10 渭 m ~ 100 渭 m, and the particle size is 0.5 渭 m ~ (10 渭 m) after ball milling. After strontium was doped with 尾 -TCP, the substitution solid solution was formed, but the content of strontium had little effect on the crystal structure. The powder has no cytotoxicity. Calcium sulfate / strontium doped 尾 -TCP composite pellets were prepared by agitation spray drying method. That is, when the amount of CaSO_4 powder, the concentration of binder, the concentration of strontium 尾 -TCP and the proportion of strontium 尾 -TCP to CaSO_4 powder are 2.5g / 10wt% respectively. The composite pellets with pelletizing rate of about 75% and diameter of 0.95-1.35mm could be prepared at 10 wt% and 30wt%. The cytotoxicity results showed. The cytotoxicity of the granulosa is grade 0 or grade 1, that is, there is no cytotoxicity. Using silica gel, gelatin and sodium carboxymethyl cellulose, porous sio _ s _ 2 was prepared by stacking and aggregating. The results showed that the porosity of the three composite scaffolds was about 60%; The three kinds of composite scaffolds all have irregular pore structure, and the pore size between the small sphere and the small sphere is between 0.2-1.Omm. The pore sizes of SiO2 / composite scaffolds were mainly distributed in 100-300 渭 m. The pore sizes of gelatin / composite pellet scaffolds were mainly distributed in 100-500 渭 m. The average compressive strength of carboxymethyl cellulose sodium / composite pellet scaffold was about 0.1 MPA, which was mainly distributed in 50-300 渭 m.SiO2 / composite scaffold. The average compressive strength of gelatin / composite microsphere scaffold was 6.3 Mpa.The average compressive strength of carboxymethyl cellulose sodium / composite microsphere biological scaffold was 0.7 Mpa. In vitro biodegradation experiments showed that there was apatite deposition in the process of immersion in SBF of the three scaffolds, which indicated that the scaffolds had good bioactivity. Among them, the degradation cycle of gelatin / composite scaffolds was the longest. The degradation period of carboxymethyl cellulose sodium / composite pellet scaffold was shorter, about 40 days. The biodegradation of SiO2 / composite scaffolds was basically complete on the 80th day of the experiment. The cytotoxicity of the three composite scaffolds was grade 0 or grade 1, that is, no cytotoxicity.
【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB332;R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孫猛;鄭東翔;夏揚(yáng);;聚乳酸-羥基乙酸/磷酸三鈣人工骨的體外構(gòu)建[J];軍事醫(yī)學(xué);2013年12期

2 趙天源;孫紅;;骨組織工程支架材料及其血管化的研究進(jìn)程[J];中國組織工程研究;2013年38期

3 陳博;魯圣軍;于杰;龔維;郭建兵;張純;;POE/EVA復(fù)合發(fā)泡材料性能[J];塑料;2012年05期

4 郭睿;農(nóng)曉琳;;復(fù)合支架材料在口腔骨組織工程領(lǐng)域的研究與進(jìn)展[J];口腔生物醫(yī)學(xué);2012年03期

5 劉昊;張永剛;;骨組織工程的研究應(yīng)用與進(jìn)展[J];生物骨科材料與臨床研究;2012年02期

6 劉順振;侯玉東;;骨組織工程支架材料的研究進(jìn)展及臨床應(yīng)用[J];中國組織工程研究與臨床康復(fù);2011年42期

7 徐麗莎;劉文晶;申少波;楊照;陳蕊蕊;韓英;尹芳;時(shí)永全;周新民;;蠶絲蛋白和明膠復(fù)合組織工程材料與肝細(xì)胞相容性的實(shí)驗(yàn)研究[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2011年08期

8 劉家祺;亓發(fā)芝;;Beta-磷酸三鈣復(fù)合材料支架的研究進(jìn)展[J];中國美容醫(yī)學(xué);2011年04期

9 劉遠(yuǎn)平;張琳琳;張慧怡;趙丹;馬然;汪秋寬;;海燕體壁膠原蛋白提取工藝的初步研究[J];大連海洋大學(xué)學(xué)報(bào);2011年01期

10 鄒景良;張躍;;冷凍干燥法工藝條件對多孔氧化鋁陶瓷的影響[J];稀有金屬材料與工程;2009年S2期

，

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