發(fā)布時間：2018-01-27 05:24

  本文關(guān)鍵詞： 金/銀納米簇 乙酰膽堿酯酶 重金屬離子 苯硼酸 糖類多羥基化合物 化學(xué)與生物傳感技術(shù)　出處：《曲阜師范大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：貴金屬納米簇(NCs),如金納米簇(AuNCs)、銀納米簇(AgNCs)及其雙金屬納米簇(Au/AgNCs)具有獨(dú)特的化學(xué)和物理性能,被廣泛的應(yīng)用于化學(xué)與生物傳感器中,實(shí)現(xiàn)了對多種目標(biāo)物的檢測。本論文一方面通過合成強(qiáng)熒光金銀納米簇,利用待測物質(zhì)對其熒光的猝滅效應(yīng),建立了有機(jī)磷農(nóng)藥和重金屬離子的傳感檢測體系;同時,借助苯硼酸對多羥基化合物的特異識別特性,發(fā)展了一種糖類等多羥基化合物的目視高度速測技術(shù)。(1)通過合成強(qiáng)熒光金納米簇為探針,建立了一種基于“一滴溶液”免標(biāo)記的熒光分析方法,用于蔬菜中有機(jī)磷殘留物的分析(第二章)。該方法借助乙酰膽堿酶(AChE)催化硫代乙酰膽堿水解產(chǎn)生硫代膽堿引起金納米簇的團(tuán)聚,進(jìn)而導(dǎo)致其熒光猝滅的特性,并利用敵敵畏(DDVP)對乙酰膽堿酶(AChE)催化活性的抑制效應(yīng),實(shí)現(xiàn)了對農(nóng)藥殘留的快速、靈敏、特異的檢測(線性范圍0.032 nM-20 nM,檢出限13.67 pM),其對實(shí)際蔬菜樣品中殘留DDVP的檢出限達(dá)36 pM。該熒光分析方法通過速測有機(jī)磷感染的直接標(biāo)志物自由有機(jī)磷,實(shí)現(xiàn)了對有機(jī)磷早期感染的預(yù)警,并為酶生理催化活性的評價提供了一種新思路。(2)采用一鍋反應(yīng)的生物礦化合成路線,并通過調(diào)整金/銀前驅(qū)體的摩爾比例,合成了強(qiáng)熒光的雙金屬金銀合金納米簇,用以作為熒光探針實(shí)現(xiàn)了對血液中的銅離子和汞離子的快速、特異、超靈敏的檢測(第三章)。研究表明,制備的金銀合金納米簇具有“銀效應(yīng)”增強(qiáng)的紅色熒光,其熒光強(qiáng)度分別是常見金納米簇和核-殼結(jié)構(gòu)的金銀納米簇的6.5倍和4.7倍;特別是,“銀效應(yīng)”不僅提高了金銀合金納米簇響應(yīng)汞離子的能力,而且賦予了其特異檢測銅離子的能力;此外,通過引入銅離子絡(luò)合劑,實(shí)現(xiàn)了對銅離子和汞離子的分別檢測;所構(gòu)建的熒光分析法檢測血液中汞離子和銅離子的檢測限分別達(dá)0.30 nM和0.60 nM,可望應(yīng)用于臨床實(shí)驗(yàn)室中血汞和血銅的檢測。(3)基于功能化苯硼酸衍生物對糖類等多羥基化合物的特異識別原理以及毛細(xì)效應(yīng)建立了一種目視高度的速測技術(shù),用于血液、天然產(chǎn)物、農(nóng)產(chǎn)品以及化工產(chǎn)品中單糖、多糖以及含有1,2-或1,3-二醇基團(tuán)的多羥基化合物的檢測(第四章)。將功能化苯硼酸衍生物修飾于預(yù)先氨基硅烷化處理的刻度毛細(xì)管內(nèi)壁,通過毛細(xì)效應(yīng)自動吸入樣品,進(jìn)而利用壁上苯硼酸與含有1,2-或1,3-二醇基團(tuán)的多羥基化合物形成復(fù)合物后發(fā)生溶脹,導(dǎo)致毛細(xì)管內(nèi)壁的親疏水性的改變,進(jìn)而引起刻度毛細(xì)管內(nèi)液面高度的規(guī)律性上升,經(jīng)目測其液面高度實(shí)現(xiàn)對待測樣品中糖類等多羥基化合物含量的速測。該方法以功能化苯硼酸衍生物作為糖類等多羥基化合物的識別體,克服了傳統(tǒng)酶學(xué)檢測技術(shù)存在的諸多缺點(diǎn)(例如,酶失活問題),具有樣品用量少(約20μL)、操作簡單、價格低廉、適于現(xiàn)場速測等優(yōu)點(diǎn)。
[Abstract]:Noble metal nanoclusters (NCs), such as gold nanoclusters (AuNCs), silver nanoclusters (AgNCs) and double metal nanoclusters (Au/AgNCs) with unique chemical and physical properties, is widely used in chemical and biological sensor, the detection of multiple targets. The article on the one hand, through the synthesis a strong fluorescence quenching effect of silver nanoparticles, using the analytes on the fluorescence, a sensing system of organophosphorus pesticide and heavy metal ions; at the same time, with the specific recognition properties of phenylboronic acid of polyhydroxy compounds, the development of a sugar polyol. Rapid detection of visual height (1) by synthesis of strong fluorescent gold nanoclusters as probe, was established based on the "one drop solution" label free fluorescence analysis method for the analysis of organic phosphorus residues in vegetables (chapter second). This method uses acetylcholine enzyme (AChE) catalyzes the thioacetyl Choline is hydrolyzed to produce thiocholine by gold nanoclusters agglomeration, leading to the characteristics of fluorescence quenching, and the use of dichlorvos (DDVP) on acetylcholinesterase (AChE) inhibitory effect on catalytic activity, the pesticide residue rapid, sensitive and specific detection (linear range of 0.032 nM-20 nM, the detection limit is 13.67 pM). The actual DDVP residues in vegetable samples detection limit of 36 pM. the fluorescence analysis method by measuring the direct signs of infection of the organic phosphorus free organic phosphorus, organic phosphorus for infection early warning, and provides a new method for evaluation of physiological enzyme catalytic activity. (2) biomineralization synthesis route the one pot reaction, and through adjusting the molar ratio of gold / silver precursor, double metal alloy of gold and silver nanoclusters strong fluorescence were synthesized and used as fluorescent probes for mercury ion and copper ion in the blood quickly, especially ISO, ultra sensitive detection (the third chapter). The research results show that the alloy of gold and silver nanoclusters prepared with red fluorescent silver enhancement effect, the fluorescence intensity was 6.5 times gold and silver nanoclusters common gold nanoclusters and core-shell structure and 4.7 times; in particular, the "silver" effect not only to improve the response ability of gold and silver alloy nanocluster mercury ion, and gives the specific detection of copper ions; in addition, through the introduction of copper ion complexing agent, the detect of copper ions and mercury ion; fluorescence detection analysis of blood mercury ion and copper ion limit of 0.30 nM and 0.60 nM respectively, the detection can be used in clinical laboratory blood mercury and blood copper. (3) functionalized phenylboronic acid derivatives of saccharide polyhydroxy compounds and the specific recognition principle is established based on the capillary effect of a high speed visual measurement technology, with In the blood, natural products, agricultural products, chemical products, monosaccharide, polysaccharide and detection of polyhydroxy compounds containing groups of 1,2- or 1,3- of the diol (Chapter fourth). The function of phenylboronic acid derivatives modified on the inner wall of capillary amino silane treatment scale in advance, automatic suction samples by capillary effect, and then by swelling on the wall of phenylboronic acid with polyhydroxy compounds containing groups of 1,2- or 1,3- glycol to form compound, lead to the inner wall of the capillary wettability change, thereby causing regular scale capillary level in the rise of the visual realization of the height of liquid level detecting sugars in samples of polyhydroxy compounds content. The method of velocity measurement the function of phenylboronic acid derivatives as recognition sugar polyol, overcomes many disadvantages of traditional enzymatic detection techniques (e.g., enzyme inactivation problem), out There are few samples (about 20 L), simple operation, low price, and suitable for field rapid measurement.

【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：O657.3;TB34

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides[J];Science China(Chemistry);2010年04期

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本文編號：1467688