本文關(guān)鍵詞： 血紅素蛋白 分離 聚合修飾 磁性粒子　出處：《北京化工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：在生命科學(xué)的領(lǐng)域中,對(duì)于蛋白質(zhì)進(jìn)行選擇性分離和檢測(cè)都具有重要的意義。磁性材料的引入使得蛋白質(zhì)的分離技術(shù)發(fā)展迅速,其快速高效的分離能力得到了研究者們的廣泛青睞,越來越多的蛋白質(zhì)分離研究?jī)A向于選用磁性材料作為載體。通過磁性材料表面的修飾基團(tuán)和蛋白質(zhì)中的目標(biāo)位點(diǎn)進(jìn)行結(jié)合就可實(shí)現(xiàn)分離。本論文主要采用聚合方法對(duì)于磁性載體進(jìn)行修飾并對(duì)其在蛋白質(zhì)分離中的能力進(jìn)行研究。在本論文研究中,目的是將磁性材料和聚合物材料的優(yōu)勢(shì)結(jié)合起來,合成一種高效分離血紅素蛋白質(zhì)材料。通過自由基聚合的方法,將具有咪唑環(huán)的涂層接枝在磁性粒子表面,通過咪唑環(huán)與血紅素蛋白中鐵的配位作用達(dá)到分離蛋白質(zhì)的目的。通過一系列的表征對(duì)于所合成材料的結(jié)構(gòu)和組成進(jìn)行了研究,例如透射電子顯微鏡,傅里葉變換紅外光譜和熱重分析等。結(jié)果表明,聚乙烯基咪唑改性的磁性微米顆粒(PVIM-MMPs)在磁場(chǎng)中表現(xiàn)出強(qiáng)烈的磁響應(yīng),對(duì)于血紅蛋白有較高的吸附能力(吸附量為6.96 g/g),較好的選擇性(對(duì)比了非血紅素蛋白質(zhì)溶菌酶)和良好的可回收性。此外,研究中還制備了一種由聚乙烯基咪唑修飾的磁性納米顆粒(PVIM-MNPs),并對(duì)其蛋白分離能力進(jìn)行了考察,通過一系列的表征及測(cè)試,結(jié)果表明聚合物修飾的納米顆粒表現(xiàn)出更好的吸附能力,對(duì)于未來生物大分子的分離載體提供了選擇。綜上,論文中建立的聚合方法所制備的材料對(duì)于血紅素蛋白的分離具有選擇性,分離方法簡(jiǎn)化了各種前處理,分離效率高,操作簡(jiǎn)便,可以快速實(shí)現(xiàn)磁性粒子的聚集并從母液分離,這一技術(shù)將有助于為蛋白質(zhì)組學(xué)提供高豐度蛋白質(zhì)去除技術(shù),或低豐度蛋白質(zhì)的富集研究,特別是針對(duì)血紅素蛋白的分離純化研究方面有很好的意義。
[Abstract]:In the field of life science, the selective separation and detection of proteins are of great significance. The introduction of magnetic materials makes the rapid development of protein separation technology. Its rapid and efficient separation ability has been widely favored by researchers. More and more researches on protein separation tend to use magnetic materials as carriers. The separation can be realized by binding the modified groups on the surface of magnetic materials and the target sites in proteins. Methods to modify the magnetic carrier and study its ability in protein separation. The aim of this study was to combine the advantages of magnetic materials with polymer materials to synthesize a highly efficient heme protein separation material. The coating with imidazole ring was grafted onto the surface of magnetic particles by free radical polymerization. The protein was isolated by the coordination between imidazole ring and iron in heme protein. The structure and composition of the synthesized materials were studied by a series of characterization, such as transmission electron microscopy. Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The results show that PVIM-MMPsmodified by polyvinylimidazolium has a strong magnetic response in the magnetic field. High adsorption capacity for hemoglobin (6.96 g / g), better selectivity (compared with non-heme protein lysozyme) and good recoverability. A kind of magnetic nanoparticles PVIM-MNPsN modified by polyethylene imidazole was prepared and its protein separation ability was investigated. A series of characterization and tests were carried out. The results show that polymer modified nanoparticles exhibit better adsorption ability and provide a choice for the separation of biomolecules in the future. The materials prepared by the polymerization method in this paper have selectivity for the separation of heme protein. The separation method simplifies all kinds of pretreatment, and the separation efficiency is high and the operation is simple. Magnetic particles can be rapidly aggregated and separated from mother liquor. This technique will be helpful to provide proteomics with high abundance protein removal technology or low abundance protein enrichment research. In particular, the study on the separation and purification of heme proteins is of great significance.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O631;TB383.1

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 田愛林;功能化磁性納米粒子的合成及其手性分離的應(yīng)用研究[D];北京化工大學(xué);2013年

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本文編號(hào)：1460100