本文關(guān)鍵詞： 碳點 纖維素 生物成像 Fe(Ⅲ)檢測　出處：《浙江理工大學》2017年碩士論文　論文類型：學位論文

【摘要】：碳點(CDs)是一種尺寸小于10 nm的新型熒光納米粒子,自2004年CDs被報道以來,已經(jīng)吸引了廣大科研工作者的興趣和關(guān)注。CDs具有極好的生物相容性、優(yōu)秀的熒光性能、穩(wěn)定的化學和物理性質(zhì)、低毒性、極佳的水溶性等性質(zhì),大大拓展了CDs的應用領(lǐng)域和范圍。目前,CDs的制備方法主要有,激光蝕刻法,電化學法,模板法等,這些方法存在原料價格高,設(shè)備價格昂貴或者實驗操作復雜等缺陷,因此,急需一種操作簡單,原料便宜同時可提高碳點熒光量子產(chǎn)率的制備方法。提高CDs熒光量子產(chǎn)率的方法一般有兩種,一種是后期對CDs進行表面鈍化;另一種是摻雜雜原子。為了進一步降低制備成本,改善CDs的熒光性能,本課題選擇以廉價易得可降解的生物質(zhì)基—纖維素為原料,摻雜氮硫雜原子,通過一步水熱法制得一系列纖維素基熒光CDs,分別對制得的CDs進行最適實驗條件探究,形貌表征,成分和性能分析,通過細胞毒性試驗評價其毒性,并將它們應用于生物成像與在溶液中對Fe(Ⅲ)檢測,并對Fe(Ⅲ)檢測下限和檢測機理進行探究最終得到實驗結(jié)論如下:(1)氮摻雜纖維素基熒光CDs的制備及在生物成像的應用研究:以纖維素粉和尿素為原料,通過綠色環(huán)保的水熱法一步制備了氮摻雜纖維素基熒光CDs(N-CDs)�？疾炝四蛩刭|(zhì)量、反應時間和反應溫度對N-CDs熒光量子產(chǎn)率的影響,并探究最適實驗條件為:尿素與纖維素的質(zhì)量比為0.7,反應溫度為180°C,反應時間為72 h,此時N-CDs的熒光量子產(chǎn)率達21.7%;對N-CDs的形貌、成分和光學性能進行表征與分析,發(fā)現(xiàn)它的平均尺寸為4.2 nm,表面存在氨基、羥基等基團,具有依賴激發(fā)光波長的熒光性質(zhì);通過細胞毒性試驗,發(fā)現(xiàn)N-CDs的細胞存活率達89%以上,并將其成功應用于細胞成像。(2)氮硫共摻雜纖維素基熒光CDs的制備及在生物成像的應用研究:以纖維素粉、硫代硫酸鈉和乙二胺(EDA)為原料,通過綠色環(huán)保的水熱法一步制備了氮硫共摻雜纖維素基熒光CDs(N,S-CDs)。N,S-CDs的最適實驗條件為:纖維素粉0.5 g,硫代硫酸鈉0.1 g,乙二胺0.5 mL,反應時間72 h,反應溫度180°C,此時他的熒光量子產(chǎn)率達28.2%;并對N,S-CDs的形貌、成分和光學性能進行表征與分析,發(fā)現(xiàn)它的平均尺寸為3.2 nm,存在氨基,羥基和含硫基團等基團,具有依賴激發(fā)光波長的熒光性質(zhì);相較于N-CDs,N,S-CDs具有更高的熒光量子產(chǎn)率,相同激發(fā)光下的N,S-CDs發(fā)射光發(fā)生紅移;通過細胞毒性試驗,發(fā)現(xiàn)N,S-CDs的細胞存活率達93%以上,并將其成功應用于細胞成像。(3)纖維素基熒光CDs在溶液中對Fe(Ⅲ)檢測的應用研究:將Cellulose-CDs,N-CDs,N,S-CDs和EDA-CDs分別在PBS緩沖液和水溶液當中進行Fe(Ⅲ)檢測。并對N,S-CDs和EDA-CDs分別在PBS緩沖液和水溶液中進行Fe(Ⅲ)檢測下限的測定,分別為0.3119 ppm和0.03378 ppm,對其檢測機理進行探究。
[Abstract]:Carbon dots (CDs) is a new type of fluorescent nanoparticles with size less than 10 nm, which has been reported since 2004. CDs have attracted the interest and attention of many researchers. CDs have excellent biocompatibility, excellent fluorescence properties, stable chemical and physical properties, low toxicity, excellent water solubility and so on. At present, the main preparation methods of CDs are laser etching, electrochemical method, template method and so on. These methods have high raw material price. The equipment is expensive or the experiment operation is complex, so it is urgent to have a simple operation. The method of preparing carbon dot fluorescence quantum yield can be improved by cheap raw material. There are two methods to improve CDs fluorescence quantum yield, one is to passivate the surface of CDs in the later stage; In order to further reduce the cost of preparation and improve the fluorescence properties of CDs, we choose biodegradable biomass-based cellulose as the raw material, doped nitrogen and sulfur hetero atoms. A series of Cellulose based fluorescent CDs were obtained by one step hydrothermal method. The optimal experimental conditions, morphology characterization, composition and performance analysis of the CDs were studied, and the toxicity was evaluated by cytotoxicity test. They were applied to biometric imaging and detection of Fe (鈪，

本文編號：1450466