本文關(guān)鍵詞：新型碳納米復合材料在大環(huán)內(nèi)酯類和酚類藥物電化學檢測中的應用　出處：《江西科技師范大學》2015年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 電化學傳感器 大環(huán)內(nèi)酯類藥物 酚類藥物 碳納米材料 聚(3 4-乙撐二氧噻吩)

【摘要】：在藥物分析領(lǐng)域,其傳統(tǒng)的分析方法主要為生物分析法、電泳法、熒光光譜法、紫外光譜法、高效液相色譜法、氣相色譜法、色譜與質(zhì)譜聯(lián)用法等,這些方法已經(jīng)成為藥物分析領(lǐng)域中的主流,在檢測藥物有效成分以及藥物殘留等方面得到了廣泛地應用。然而,盡管這些技術(shù)在檢測靈敏度等方面得到了廣泛的認可,但是由于該類方法在進行分析之前往往需要對樣品進行預處理,在分析檢測的過程中需要專業(yè)的技術(shù)人員進行操作,以及本身儀器價格昂貴、體積較大等原因,使得這一類方法很難實現(xiàn)樣品的在線檢測與分析。而電化學傳感器由于其具有簡便、快捷、靈敏度高而且能夠?qū)崿F(xiàn)對分析物的在線檢測與分析越來越受到人們的關(guān)注。因此,電化學傳感在藥物分析中的優(yōu)越性也越來越得到人們的認可。本論文工作在綜合文獻報道的基礎(chǔ)上,針對多壁碳納米管(MWCNTs)和氧化石墨烯(GO)擁有高的比表面積、獨特空間結(jié)構(gòu)和易于功能修飾等特點,緊緊圍繞碳納米管和氧化石墨烯這兩種碳納米增強的復合物構(gòu)建了一系列的大環(huán)內(nèi)酯類和酚類藥物電化學傳感器,具體內(nèi)容如下：(1)利用簡單的超聲的方法制備了氧化石墨烯-多壁碳納米管(GO-MWCNTs)復合材料。通過透射電鏡、拉曼光譜、紅外光譜、紫外光譜、電化學阻抗等手段對材料進行了表征,發(fā)現(xiàn)MWCNTs可以與GO進行很好地結(jié)合,并且GO會吸附到MWCNTs的表面形成項鏈狀的形貌結(jié)構(gòu)。該復合材料進一步被應用于大環(huán)內(nèi)酯類藥物-阿奇霉素電化學傳感器的構(gòu)建。電化學數(shù)據(jù)顯示,與裸玻碳電極、GO以及MWCNTs修飾的電極相比,GO-MWCNTs修飾的電極對阿奇霉素顯示出較大的氧化峰電流。在最佳條件下氧化峰的大小與被測阿奇霉素的濃度成正比,且其線性范圍是0.1～10μM,最低檢測限是0.07μM。為了進一步探索其在實際樣品中的應用,GO-MWCNTs修飾電極構(gòu)建的傳感平臺成功被用于阿奇霉素藥片中阿奇霉素含量的測定,并取得了滿意的效果。(2)通過界面聚合的方法制備了氧化石墨烯／聚(3,4-乙撐二氧噻吩)(GO/PEDOT)復合材料,利用透射電鏡、紅外光譜、拉曼光譜、電化學阻抗等手段對材料進行了表征,結(jié)果證實PEDOT可以與GO很好地復合在一起。最后將GO/PEDOT復合材料修飾的電極應用于蘆丁電化學傳感器的構(gòu)建,實現(xiàn)了對蘆丁的分析檢測,并進一步提出了蘆丁可能的氧化機理。在最佳條件下,電極對蘆丁氧化峰的電流大小與蘆丁的濃度呈線性相關(guān)關(guān)系,其檢測的線性范圍是0.004～60μM,其最低檢測限可以達到0.00125μM,而且該電化學傳感器實現(xiàn)了實際蘆丁藥品中蘆丁含量的分析與檢測。(3)與GO相比,基于MWCNTs納米材料修飾的電極對某些藥物分子可以顯示出更強的催化作用,因此利用界面聚合的方法同時制備了多壁碳納米管／聚(3,4-乙撐二氧噻吩)(MWCNTs/PEDOT)復合材料,利用透射電鏡、紅外光譜、拉曼光譜、電化學阻抗以及電量—時間曲線等手段對材料進行了表征,結(jié)果表明PEDOT膜可以成功地吸附在MWCNTs的表面。最后,將MWCNTs/PEDOT復合材料應用于厚樸酚電化學傳感器的構(gòu)建。通過對檢測時實驗條件進行優(yōu)化,確立了該電化學傳感器在pH值等于7.0時對厚樸酚具有最佳的檢測效果,其檢測的線性范圍是0.01～25μM,最低檢測限可以達到0.003μM,該電化學傳感器可以應用于厚樸提取物中厚樸酚含量的測定。(4)利用一步電化學的方法,將具有納米結(jié)構(gòu)的GO-MWCNTs作為摻雜劑導入PEDOT的基體中,制備了(GO-MWCNTs/PEDOT復合材料,并利用該復合材料構(gòu)建了己烯雌酚電化學傳感器。通過對構(gòu)建的該電化學傳感器實驗參數(shù)的優(yōu)化可知,當緩沖溶液的pH值為7.0,富集時間為120s時,該電化學傳感器對己烯雌酚具有最佳的檢測效果,其檢測的線性范圍是0.01～20μM,最低檢測下限為0.003μM,而且該電化學傳感器可以應用于己烯雌酚片中己烯雌酚的含量分析與檢測。
[Abstract]:In the field of drug analysis, the traditional analysis method for biological analysis, electrophoresis, fluorescence spectroscopy, UV spectroscopy, high-performance liquid chromatography, gas chromatography, gas chromatography and mass spectrometry, these methods have become the mainstream in the field of drug analysis, has been widely used in detection of the active ingredient of the drug and drug residue. However, although these techniques in detection sensitivity have been widely recognized, but because this kind of method needs sample pretreatment often before analysis, in the process of analysis of professional and technical personnel to operate, and its equipment is expensive, volume big and other reasons, makes this method very difficult to realize the on-line detection and analysis of samples. And the electrochemical sensor because of its simple, fast, high sensitivity and can realize the analysis of matter The online detection and analysis has attracted more and more attention. Therefore, electrochemical sensing superiority in pharmaceutical analysis is more and more recognized by people. The work in this thesis is based on the literatures on the multi walled carbon nanotubes (MWCNTs) and graphene oxide (GO) has high specific surface area. The unique spatial structure and easy modification and other characteristics, closely around the complexes of carbon nanotubes and graphene oxide of the two carbon nano enhanced constructed a series of macrolide drugs and phenol electrochemical sensor, the specific contents are as follows: (1) graphene oxide / multi walled carbon nanotubes were synthesized by a simple method the ultrasound system (GO-MWCNTs) composite material. By transmission electron microscopy, Raman spectroscopy, infrared spectroscopy, UV spectroscopy, the materials were characterized by electrochemical impedance method, found that MWCNTs can be well combined with GO and GO. Will be adsorbed to the morphology and structure formation of necklace like the surface of the MWCNTs. The composite material is further applied to macrolide drug azithromycin electrochemical sensor was constructed. The electrochemical data show that compared with the bare glassy carbon electrode, GO electrode and MWCNTs modified electrode, GO-MWCNTs modified to azithromycin showed the oxidation peak current in larger. The concentration of the oxidation peak under optimum conditions and measured the size of azithromycin is proportional, and the linear range is 0.1 ~ 10 M, the minimum detection limit is 0.07 M. in order to further explore its application in actual sample, sensing platform to construct a GO-MWCNTs modified electrode was successfully used for the determination of azithromycin tablets and azithromycin. Have achieved satisfactory results. (2) by interfacial polymerization method to prepare graphene oxide / poly (ethylene oxide 3,4- two thiophene) (GO/PEDOT) composite materials, using transmission power Mirror, infrared spectroscopy, Raman spectroscopy, the materials were characterized by electrochemical impedance method, the results demonstrated that PEDOT and GO can be well combined together. Finally build the GO/PEDOT composite modified electrode applied to rutin electrochemical sensor, realizes the analysis of detection of rutin, and further puts forward the mechanism of oxidation of rutin may. Under the optimum conditions, a linear correlation relationship between the concentration of rutin oxidation peak current of the electrode size and rutin, 0.004 to the detection linear range is 60 M, the lowest detection limit can reach 0.00125 M, and the electrochemical sensor realizes the detection and analysis of the actual content of Rutin in drug (rutin. 3) compared with GO, MWCNTs modified electrodes of nanometer materials can be of some drug molecules showed stronger catalysis based on the method of interfacial polymerization and preparation of multi walled carbon nanotubes were prepared Poly (ethylene / 3,4- two oxygen thiophene) (MWCNTs/PEDOT) composite materials, using transmission electron microscopy, infrared spectroscopy, Raman spectroscopy, the materials were characterized by electrochemical impedance and power time curve method, the results show that the PEDOT film can be successfully adsorbed on the surface of MWCNTs. Finally, the construction of MWCNTs/PEDOT composite used in Magnolia officinalis the phenol electrochemical sensor. Based on the experimental conditions of detection were optimized, established the electrochemical sensor in pH with value equal to the optimal detection performance of magnolol from 0.01 to 7, the linear range of detection is 25 M, the lowest detection limit can reach 0.003 M, the electrochemical sensor can be applied to the determination of magnolol the content of magnolia bark extract in. (4) using the method of one step electrochemical, with nano GO-MWCNTs as matrix dopants into PEDOT in the preparation of the composite material (GO-MWCNTs/PEDOT And, using the composite material constructed Diethylstibestrol electrochemical sensor. By optimizing the experimental parameters of the electrochemical sensor constructed, when the pH value of buffer solution was 7, the accumulation time is 120s, the electrochemical sensor has the best detection effect of diethylstilbestrol, 0.01 to the linear range of detection 20 M, the minimum detection limit of 0.003 M, content analysis and detection and the electrochemical sensor can be applied to Diethylstilbestrol Tablets in diethylstilbestrol.

【學位授予單位】：江西科技師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：O657.1;TB33

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