本文關鍵詞：靜電紡絲法制備納米羥基磷灰石基生物材料及其性能研究　出處：《暨南大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 羥基磷灰石 靜電紡絲 納米材料 水熱法 復合材料 生物相容性

【摘要】：羥基磷灰石(hydroxyapatite,Ca5(PO4)3OH,Hap)擁有良好的生物相容性,以及優(yōu)異的表面生物吸附活性,其在生物醫(yī)學領域有著廣泛的應用。至少一維上尺度小于100 nm的納米Hap有著極佳生物學性能。本研究的目的是通過新型的方法合成的納米Hap,并對其合成機理進行探討。首先,通過水熱法以檸檬酸鈉與十六烷基三甲基溴化銨(CTAB)為調控劑,研究大小有機分子對Hap晶體形貌的影響。制備出了長200~500nm,寬20~50nm的片狀Hap晶體;長約100~200 nm,直徑約10~20 nm的細針狀Hap晶體及長約為50~100 nm直徑約為10~20nm的短棒狀晶體。研究表明,CTAB(大分子)膠束為控制Hap晶體形貌的模板;小分子檸檬酸鈉對Hap的結晶有抑制作用。當反應體系中同時存在CTAB與檸檬酸鈉作為調控劑時,CTAB對Hap晶體的形貌起主要的模板作用。采用靜電紡絲法,以磷灰石溶膠-凝膠前驅體復合高分子溶液制備出纖維直徑在100nm以下的Hap納米陶瓷纖維。纖維直徑為80~110nm,由顆粒直徑為20~40 nm的Hap晶粒聚集成多孔形態(tài)。調控Hap前驅體濃度與熱處理溫度可改變Hap纖維的形貌。Hap纖維直徑隨前驅體含量的增加而增加,隨熱處理溫度的提高而減少;高前驅體濃度可導致Hap纖維形成多孔結構。以牛血清蛋白(BSA)為蛋白質模型藥物,考察Hap纖維對BSA載藥性能。Hap的載藥量隨纖維前驅體含量和熱處理溫度的升高而降低;BSA的緩釋可持續(xù)18天以上。在Hap纖維對成骨細胞活性及ALP活性檢測的分析中,Hap纖維表現(xiàn)出對成骨細胞的生長的抑制作用但同時對ALP表達的促進作用。通過靜電紡絲法,將Ca P溶膠與聚(丙交酯-乙交酯)共聚物(PLGA)共混紡絲并進行原位礦化,探索一種有機無機復合材料的新型制備方法。成功制備了鈣磷元素分布均勻的PLGA-Ca P前驅體共混復合纖維。Ca P溶膠的加入使纖維形貌發(fā)生了纏結、分支,纖維直徑增大。之后通過氨化堿性處理,制備出Hap結晶度較低的PLGA/Hap復合纖維。經(jīng)處理后,復合材料的力學性能提高,但斷裂伸長率降低。細胞實驗表明,PLGA/Hap復合纖維對于MC3T3前成骨細胞的生長與ALP表達的促進作用。
[Abstract]:Hydroxyapatite has good biocompatibility and excellent surface biosorption activity. It has been widely used in biomedical field. At least one dimensional nanometer Hap with upper scale less than 100nm has excellent biological properties. The purpose of this study is to synthesize nanometer Hap by a new method. Firstly, sodium citrate and cetyltrimethylammonium bromide (CTAB) were used as regulators by hydrothermal method. The effect of organic molecules on the morphology of Hap crystal was studied. A Hap crystal with a length of 200 ~ 500 nm and a width of 20 ~ 50 nm was prepared. The thin needle-like Hap crystals with a diameter of 10 ~ 20 nm and a short rod crystal with a diameter of about 50 ~ 100 nm and a diameter of about 10 ~ 20 nm were obtained. CTAB micelles are templates for controlling the morphology of Hap crystals. The crystallization of Hap was inhibited by small molecule sodium citrate. When CTAB and sodium citrate were used as regulators in the reaction system. CTAB plays an important role as template for the morphology of Hap crystal. Electrostatic spinning method is used. Hap nanoceramic fibers with fiber diameter less than 100nm were prepared from apatite sol-gel precursor composite polymer solution with the fiber diameter of 80,110nm. The diameter of the particle is 20 ~ 40. The morphology of Hap fiber can be changed by adjusting the concentration of Hap precursor and heat treatment temperature. The diameter of Hap fiber increases with the increase of precursor content. It decreases with the increase of heat treatment temperature. High concentration of precursor resulted in the formation of porous structure of Hap fibers. BSA was used as a protein model drug. The drug loading capacity of Hap fiber on BSA decreased with the increase of precursor content and heat treatment temperature. The sustained release of BSA lasted more than 18 days. In the analysis of the activity of Hap fiber to osteoblast and ALP activity. Hap fibers can inhibit the growth of osteoblasts but at the same time promote the expression of ALP. Capp sol and poly (lactide-glycolide) copolymer (PLGA) were blended and spun and in situ mineralized. A new preparation method of organic-inorganic composite material was explored. The PLGA-Ca P precursor blend fiber, Ca with uniform distribution of calcium and phosphorus, was successfully prepared. The morphology of the fibers was entangled by the addition of P sol. After the branching, the diameter of the fiber increased, and then the PLGA/Hap composite fiber with low crystallinity of Hap was prepared by ammoniation and alkaline treatment. After treatment, the mechanical properties of the composites were improved. But the elongation at break decreased. Cell experiments showed that PLGA / Hap composite fibers promoted the growth and ALP expression of MC3T3 preosteoblasts.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1;TQ340.64

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