本文關鍵詞：表面分子印跡石墨烯復合材料的制備及應用　出處：《江南大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 石墨烯 表面分子印跡 傳感器 糖蛋白分離

【摘要】：表面分子印跡技術是將分子識別位點設計在載體表面或接近表面的地方,制得的表面分子印跡聚合物與傳統(tǒng)的分子印跡聚合物相比,具有目標分子更易接近印跡位點、吸附動力學更快、吸附容量更高等優(yōu)點。表面分子印跡聚合物近年來已在手性分離、固相萃取以及化學仿生傳感器等領域展現出良好的應用前景,引起了研究者們的廣泛關注。石墨烯具有大的比表面積,優(yōu)異的電、熱、機械性能,是制備表面印跡材料很好的載體。本論文對石墨烯進行修飾,成功制備出一系列表面分子印跡石墨烯復合材料,并應用于電化學傳感器和糖蛋白的特異性識別和分離。本論文具體研究內容如下:(1)通過非共價鍵修飾法獲得了乙烯基功能化的石墨烯(GR/NVC),以其為載體,以對硝基苯酚(4-NP)為模板分子、甲基丙烯酸為單體、乙二醇二甲基丙烯酸酯為交聯劑、AIBN為引發(fā)劑,制備石墨烯表面印跡復合材料(GR/MIPs)。探究了制備條件的影響,如N-乙烯基咔唑(NVC)和模板分子的濃度、溶液的p H值、孵化時間等。將石墨烯表面印跡復合材料應用于電化學傳感器的制備,制備的GR/MIPs傳感器能特異性識別和檢測4-NP,對4-NP的DPV電流響應是GR/NIPs傳感器的12倍,與傳統(tǒng)的MIP傳感器相比,GR/MIPs傳感器吸附動力學更快、電流響應值更高。GR/MIPs傳感器對4-NP的線性檢測范圍是0.01~100μM和200~1000μM,檢測下限是5 n M。與絕大多數4-NP電化學傳感器相比,GR/MIPs傳感器檢測范圍更寬,檢測下限更低,具有良好的選擇性、穩(wěn)定性和再現性,可以檢測湖水和自來水樣品中4-NP。(2)室溫下利用溶膠-凝膠技術,一鍋法水相合成氧化石墨烯分子印跡復合材料(GO/MIPs)。以氧化石墨烯(GO)為載體,撲熱息痛(PR)為模板分子,苯基三乙氧基硅烷和四甲氧基硅烷為單體,結合表面印跡技術和溶膠-凝膠法,在氧化石墨烯表面制備對PR具有特異性識別作用的分子印跡聚合物(GO/MIPs)。將GO/MIPs應用于電化學傳感器檢測PR,并探究了單體和模板分子的量及孵化時間等因素的影響。GO/MIP傳感器對PR有特異性識別能力,對PR的線性檢測范圍是0.1~80μM,檢測下限為0.02μM。與其它大多數PR電化學傳感器相比,有較寬的線性檢測范圍和較低的檢測下限。GO/MIP傳感器還具有良好的穩(wěn)定性,檢測藥片和人尿液中的PR的結果也令人滿意。(3)結合表面分子印跡、硼親和作用和溶膠-凝膠技術制備一種新型的硼酸修飾氧化石墨烯蛋白印跡復合材料(GO/APBA/MIPs),用于特異性識別和分離卵清蛋白(OVA)。探討了硅氧烷、3-氨基苯硼酸和模板蛋白的量,反應時間,緩沖溶液的p H值對復合材料識別性能的影響。通過吸附實驗發(fā)現,GO/APBA/MIPs具有吸附動力學快(60min達到吸附平衡)、吸附容量大(359.7 mg g-1)、對OVA有特異性識別能力等優(yōu)點。由于引入硼酸基團,利用其對糖蛋白的硼親和作用,使得GO/APBA/MIPs比GO/MIPs在印跡作用的基礎上,對OVA的吸附能力、特異性識別能力均得到進一步提高,可用于分離OVA。
[Abstract]:The surface molecular imprinting technique to design molecular recognition sites on the surface of the carrier or near the surface, compared to molecular imprinted polymer surface molecular imprinted polymers were prepared with the traditional, more accessible with target molecular imprinting sites, faster adsorption kinetics, adsorption capacity of more high. Surface molecular imprinted polymers in recent years has been in chiral separation. The field of solid phase extraction and chemical bionic sensor show a good application prospect, has aroused widespread concern of researchers. Graphene has a large surface area, excellent mechanical properties, electrical, thermal, carrier preparation of surface imprinted material is very good. The modification of graphene was successfully prepared a series of surface molecular imprinting graphene composites, identification and isolation of specific and applied to the electrochemical sensor and glycoproteins. The major contents in this dissertation are as follows: (1). A non covalent modification of graphene obtained vinyl functionalized (GR/NVC), with its carrier, with p-nitrophenol (4-NP) as the template molecule, methacrylic acid as monomer, ethylene glycol two methyl acrylate as crosslinking agent, AIBN as initiator, the preparation of graphene surface imprinted composite material (GR/MIPs). To explore the influence of preparation conditions, such as N- vinylcarbazole (NVC) and the concentration of the template molecule, the solution p value of H, incubation time. The application of graphene surface imprinted composite materials in electrochemical sensor preparation, GR/MIPs sensor prepared specific recognition and detection of 4-NP, DPV the current response for 4-NP is 12 times of the GR/NIPs sensor, compared with the traditional MIP sensor, faster kinetics of adsorption of GR/MIPs sensor,.GR/MIPs sensor with high current linear response to 4-NP detection range is 0.01~100 M and 200~1000 M, the detection limit is 5 N M. and the Compared to most of the 4-NP electrochemical sensor, GR/MIPs sensor to detect a wider range, lower detection limit, good selectivity, stability and reproducibility, can detect 4-NP. lake water and tap water samples (2) by sol-gel method at room temperature, one pot of water phase synthesis of graphene oxide molecular imprinting composite (GO/MIPs). The oxidation graphene (GO) as the carrier, paracetamol (PR) as the template molecule, phenyltriethoxysilane and tetramethoxysilane as monomer, combined with surface imprinting technique and sol-gel method, molecular imprinted polymer in graphene oxide surface preparation with specific recognition of PR (GO/MIPs). The application of GO/MIPs in electrochemical sensor for the detection of PR, and to explore the influence of the.GO/MIP sensor monomer and template molecule weight and incubation time and other factors have specific recognition ability of PR, the linear detection range of PR is 0.1~80 M, a detection limit of 0.02 M. compared with most other PR electrochemical sensor, a wide linear detection range and lower detection limit of.GO/MIP sensor has good stability, the results of urine pills and people in PR are satisfactory. (3) combined with the surface molecular imprinting, boron affinity and the sol-gel method to prepare a boronic acid modified graphene oxide blot new composite materials (GO/APBA/MIPs), for the specific recognition and separation of ovalbumin (OVA). To investigate the siloxane, 3- amino phenylboronic acid and template protein amount, reaction time, buffer solution p the effect of H on the recognition performance of composite materials. The adsorption experiments found that GO/APBA/MIPs has a faster adsorption kinetics (60min adsorption equilibrium), adsorption capacity (359.7 mg g-1), the advantages of the OVA specific recognition ability. Because of the introduction of boric acid group, use the The boron affinity of glycoprotein made GO/APBA/MIPs more capable of adsorbing OVA and specific recognition ability than GO/MIPs on the basis of imprinting, and it could be used to separate OVA..

【學位授予單位】：江南大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB33

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本文編號：1410972