本文關(guān)鍵詞：磁性納米探針的制備及磁共振成像應(yīng)用研究　出處：《北京化工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 磁納米流體 磁共振成像 光動(dòng)力學(xué)治療 雙功能

【摘要】：癌癥等疾病的早發(fā)現(xiàn)早治療是目前科研與臨床研究中的巨大挑戰(zhàn)。許多生物醫(yī)學(xué)成像技術(shù)的發(fā)展,如計(jì)算機(jī)X射線體外斷層掃描術(shù)(CT),光學(xué)成像,聲學(xué)成像和磁共振成像(MRI)等,已在臨床醫(yī)學(xué)癌癥等疾病診斷中得到廣泛應(yīng)用。MRI由于其高分辨、無(wú)損傷、無(wú)電離輻射等優(yōu)勢(shì)成為強(qiáng)大的診斷成像技術(shù),但高靈敏MRI成像檢測(cè)需要使用性能優(yōu)良的核磁共振成像對(duì)比劑。本論文主要圍繞磁性納米探針的設(shè)計(jì)合成及其磁共振成像應(yīng)用開(kāi)展研究。核磁共振成像造影劑不僅要有高的磁共振信號(hào),而且需要良好的生物相容性和水溶液穩(wěn)定,便于活體成像應(yīng)用。以具有良好生物相容性的聚天冬氨酸(PASP)為包覆試劑,本論文通過(guò)簡(jiǎn)單的一步水熱合成法成功制備了Fe3O4@PASP磁性納米流體。合成的Fe3O4@PASP磁性納米顆粒表面包覆的多聚氨基酸(PASP)具有豐富的羧基官能團(tuán),使得納米粒子在水溶液中可長(zhǎng)久穩(wěn)定不沉淀,并具有好的生物偶聯(lián)性,便于生物分子的偶聯(lián),可生物降解。通過(guò)調(diào)節(jié)反應(yīng)溫度可使磁性納米顆粒尺寸控制在10-13nm范圍內(nèi),且顆粒結(jié)晶性好、磁性強(qiáng)、具有高的T2加權(quán)信號(hào)響應(yīng),橫向弛豫率高達(dá)302.6S-1mM-1。適用于磁共振成像(MRI)。發(fā)展了一種簡(jiǎn)便、快捷的制備核磁共振成像對(duì)比劑的新方法,所制備的磁共振成像對(duì)比劑Fe3O4@PASP橫向弛豫率高、生物相容性好,便于生物醫(yī)學(xué)檢測(cè)應(yīng)用。為進(jìn)一步發(fā)展生物相容性好、可生物降解的核磁共振成像對(duì)比劑,在前期工作的基礎(chǔ)上,以α-乳白蛋白的水解產(chǎn)物為功能試劑,以Ca2+和Gd3+兩種金屬離子共同誘導(dǎo)α-乳白蛋白水解產(chǎn)物自組裝,形成具有磁響應(yīng)的納米球。通過(guò)對(duì)相應(yīng)實(shí)驗(yàn)條件(部分水解的乳白蛋白的量、反應(yīng)時(shí)間、pH值、Ca2+、Gd3+和光敏劑孟加拉紅(RB)的濃度等)的調(diào)控,可以控制納米球的尺寸與形貌。第一次將α-乳白蛋白作為載體來(lái)負(fù)載用于磁共振成像的Gd3+和用于光動(dòng)力學(xué)治療的光敏劑RB。合成的雙功能納米球具有非常好的生物相容性和很好的生物靶向性,并且表現(xiàn)出很好的T1加權(quán)的磁共振成像和高效的光動(dòng)力學(xué)效應(yīng)。發(fā)展了一種以蛋白為載體制備多功能核磁共振成像對(duì)比劑的新方法,拓展診斷治療一體化多功能試劑研發(fā)新思路,可望用于臨床診斷治療一體化研究。
[Abstract]:Early cancer and other diseases at early treatment is a great challenge at present and clinical research in scientific research. The development of many biomedical imaging techniques, such as X ray computer tomography (CT) in vitro, optical imaging, acoustic imaging and magnetic resonance imaging (MRI), has been in clinical medicine in the diagnosis of diseases such as cancer widely the application of.MRI due to its high resolution, no damage, no ionizing radiation has become a powerful diagnostic imaging technique, but highly sensitive MRI imaging requires the use of the excellent performance of the magnetic resonance imaging contrast research. This thesis mainly focuses on the design and synthesis of magnetic nano probe and its application in magnetic resonance imaging MRI contrast agent. Have a high magnetic resonance signal, and the need for compatibility and good biological stability in aqueous solution, for in vivo imaging applications. With good biocompatibility of polyaspartic acid (PASP) as This paper coating reagent, through a simple hydrothermal method successfully prepared Fe3O4@PASP nano magnetic fluid. The synthesis of Fe3O4@PASP magnetic nanoparticles coated on the surface of poly amino acid (PASP) with carboxyl rich, the nanoparticles can be long stable without precipitation in aqueous solution, and has good biological coupling so, the coupling of biological molecules, biodegradable. By adjusting the reaction temperature can make the magnetic particle size in the range of 10-13nm, and the particles have good crystallinity and strong magnetism, with high T2 weighted signal response of the transverse relaxation rate as high as 302.6S-1mM-1. for magnetic resonance imaging (MRI). The development of a is a new method of preparation of magnetic resonance imaging contrast agent fast, the magnetic resonance imaging contrast agent Fe3O4@PASP transverse relaxation by Henan high rate, good biocompatibility and easy application in biomedical detection. Step in the development of good biocompatibility, magnetic resonance imaging contrast agent can be biodegradable, on the basis of previous work, the hydrolysis product of alpha lactalbumin as functional reagent, induced by common alpha lactalbumin hydrolysate self-assembled by Ca2+ and Gd3+ two kinds of metal ions, formed with magnetic nanoparticles. The response of the the corresponding experimental conditions (partial hydrolysis of lactalbumin amount, reaction time, pH value, Ca2+, Gd3+ and photosensitizer Rose Bengal (RB) concentration) control, can control the size and morphology of nanoparticles. The first alpha lactalbumin as the carrier to load for magnetic resonance imaging and for Gd3+ photodynamic therapy photosensitizer RB. synthesis of bifunctional nanoparticles have good biocompatibility and good biological targeting, and showed T1 weighted magnetic resonance imaging is a good and efficient photodynamic effect. The development of a A new method of preparing multifunctional magnetic resonance imaging contrast agent based on protein and expanding the new idea of diagnosis and treatment integrated multifunctional reagents is expected to be applied in the integrative research of clinical diagnosis and treatment.

【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1

【共引文獻(xiàn)】

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1 葉偉;地衣芽孢桿菌谷氨酸特異性內(nèi)肽酶的功能特性研究[D];華南理工大學(xué);2013年

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本文編號(hào)：1377921