【摘要】：目的:探討制備冰凍血小板的關(guān)鍵技術(shù)及其臨床應(yīng)用效果。方法:回顧性分析3257份冰凍血小板機采前獻(xiàn)血員外周血小板數(shù)、開始冰凍時間、凍存保護劑注入速度和均勻度、存放形式、復(fù)融溫度和水浴箱容量等制備條件對冰凍血小板質(zhì)量的影響,通過檢測150例輸注冰凍血小板患者輸注后1、24、48和72 h外周血的血小板計數(shù)和90例產(chǎn)科出血病人輸注200份復(fù)融冰凍血小板后外周血小板數(shù)、血小板升高指數(shù)(CCI)、出血時間和血塊收縮率等指標(biāo)觀察冰凍血小板臨床應(yīng)用的效果。結(jié)果:外周血小板數(shù)為(175-250)×10~9/L獻(xiàn)血員的復(fù)融冰凍血小板絮狀物明顯減少(P0.01)。DMSO注入過快且速度不勻、血袋多層存放、小容量水浴箱復(fù)融等因素均可明顯降低冰凍血小板質(zhì)量。常規(guī)保存0和3 d再凍存對血小板功能無影響。凍存時間1年內(nèi)血小板回收率平均80%。產(chǎn)科出血病人輸注冰凍血小板后止血效果良好,未出現(xiàn)輸血反應(yīng)。冰凍血小板輸入體內(nèi)后立刻被消耗并發(fā)揮其功能,48 h后計數(shù)提升不佳。結(jié)論:機采前獻(xiàn)血員外周血小板數(shù)量、保護劑注入速度和均勻度、存放血袋層數(shù)和復(fù)融水浴箱容量等均為影響冰凍血小板質(zhì)量的關(guān)鍵因素。本研究制備的冰凍血小板臨床應(yīng)用效果良好。
[Abstract]:Objective: to investigate the key technology of frozen platelet preparation and its clinical application. Methods: 3257 frozen platelet donors were analyzed retrospectively, including the number of platelets, the starting time of freezing, the injection speed and uniformity of cryopreservation protector, and the storage form. Effects of preparation conditions such as melting temperature and bath capacity on the quality of frozen platelets, The platelet count in peripheral blood of 150 patients with frozen platelet infusion at 1 h, 24 h, 48 h and 72 h after infusion, and the number of peripheral platelets and platelet elevation index (CCI),) after infusion of 200 frozen platelets in 90 patients with obstetrical hemorrhage were measured. The effect of clinical application of frozen platelets was observed by bleeding time and clot contraction rate. Results: the number of peripheral platelets was (175 鈮，

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