流式熒光法檢測(cè)胃蛋白酶原在臨床的應(yīng)用
發(fā)布時(shí)間：2019-01-05 18:03
【摘要】：目的:評(píng)價(jià)流式熒光法在血清胃蛋白酶原(PG)I/II檢測(cè)中的臨床應(yīng)用價(jià)值。方法:1.評(píng)價(jià)流式熒光法檢測(cè)PGI/II的精密度。2.通過(guò)檢測(cè)臨床血清樣本與芬蘭Biohit集團(tuán)的Elisa試劑盒進(jìn)行方法學(xué)比對(duì)。3.評(píng)價(jià)流式熒光法檢測(cè)PGI/II的檢測(cè)限。4.驗(yàn)證流式熒光法檢測(cè)PGI/II的線性范圍。5.應(yīng)用流式熒光法檢測(cè)淺表性胃炎、萎縮性胃炎、胃癌患者的血清標(biāo)本。6.以PGI70、PGR3為診斷臨界值,來(lái)評(píng)價(jià)血清PG診斷胃癌的敏感性和特異性。結(jié)果:1.PGI檢測(cè)批內(nèi)變異系數(shù)(CV)為4.26~5.35%,批間CV為6.73~7.75%。PGII檢測(cè)批內(nèi)CV為5.48~6.42%,批間CV為8.46~8.85%。2.與ELISA方法進(jìn)行方法學(xué)比對(duì)存在線性關(guān)系,線性方程分別為PGI:Y=0.911X-22.635,R=0.966;PGII:Y=0.892X-0.548,R=0.980。3.PGI的最低檢測(cè)限為0.87 ng/ml,PGII的最低檢測(cè)限為0.46 ng/ml。4.PGI、PGII在試劑盒提供的線性范圍內(nèi)具有良好的線性(R0.99)。5.萎縮性胃炎和胃癌患者血清PGI濃度和PGI/PGII比值,明顯低于淺表性胃炎組,差異有統(tǒng)計(jì)學(xué)意義(P.0.05)。6.以PGI70ng/ml且PGR3為診斷界值,篩查胃癌的敏感性為42.1%,特異性為95.6%結(jié)論:1.流式熒光法可以實(shí)現(xiàn)PGI、PGII的并行測(cè)試,方法學(xué)性能良好,操作簡(jiǎn)便,可以滿足臨床實(shí)驗(yàn)室的需求。2.流式熒光法對(duì)血清PGI和PGII的測(cè)定可以為胃癌和萎縮性胃炎的篩查及診斷提供實(shí)驗(yàn)室依據(jù)。
[Abstract]:Objective: to evaluate the clinical value of flow-flow fluorescence assay in the detection of serum pepsinogen (PG) I/II. Methods: 1. To evaluate the precision of flow fluorescence assay for the detection of PGI/II. 2. 2. The clinical serum samples were compared with the Elisa kit of Finland Biohit Group. 3. 3. To evaluate the detection limit of PGI/II by flow fluorescence method. 4. 4. To verify the linear range of PGI/II detection by flow fluorescence method. 5. 5. Serum samples of patients with superficial gastritis, atrophic gastritis and gastric cancer were detected by flow cytometry. The sensitivity and specificity of serum PG in the diagnosis of gastric cancer were evaluated by using PGI70,PGR3 as the diagnostic threshold. Results: the coefficient of variation (CV) of 1.PGI was 4.2655.35, that of inter-assay CV was 5.48 ~ 6.42, that of 6.73~7.75%.PGII was 5.48 ~ 6.42, and that of between-assay CV was 8.46 ~ 8.85. There is a linear relationship between the method and the ELISA method. The linear equation is PGI:Y=0.911X-22.635,R=0.966;, respectively. The minimum detection limit of PGII:Y=0.892X-0.548,R=0.980.3.PGI is 0. 87 ng/ml,PGII. The minimum detection limit is 0. 46 ng/ml.4.PGI,. PGII has a good linearity in the linear range provided by the kit (R0.99). The serum PGI concentration and PGI/PGII ratio in patients with atrophic gastritis and gastric cancer were significantly lower than those in patients with superficial gastritis (P. 0. 05). With PGI70ng/ml and PGR3 as diagnostic threshold, the sensitivity and specificity of screening gastric cancer were 42.1% and 95.6% respectively. The flow fluorescence method can realize the parallel test of PGI,PGII. The method has good performance and is easy to operate, and it can meet the needs of clinical laboratory. 2. 2. The determination of serum PGI and PGII by flow fluorescence can provide laboratory basis for screening and diagnosis of gastric cancer and atrophic gastritis.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.6
本文編號(hào)：2402118
[Abstract]:Objective: to evaluate the clinical value of flow-flow fluorescence assay in the detection of serum pepsinogen (PG) I/II. Methods: 1. To evaluate the precision of flow fluorescence assay for the detection of PGI/II. 2. 2. The clinical serum samples were compared with the Elisa kit of Finland Biohit Group. 3. 3. To evaluate the detection limit of PGI/II by flow fluorescence method. 4. 4. To verify the linear range of PGI/II detection by flow fluorescence method. 5. 5. Serum samples of patients with superficial gastritis, atrophic gastritis and gastric cancer were detected by flow cytometry. The sensitivity and specificity of serum PG in the diagnosis of gastric cancer were evaluated by using PGI70,PGR3 as the diagnostic threshold. Results: the coefficient of variation (CV) of 1.PGI was 4.2655.35, that of inter-assay CV was 5.48 ~ 6.42, that of 6.73~7.75%.PGII was 5.48 ~ 6.42, and that of between-assay CV was 8.46 ~ 8.85. There is a linear relationship between the method and the ELISA method. The linear equation is PGI:Y=0.911X-22.635,R=0.966;, respectively. The minimum detection limit of PGII:Y=0.892X-0.548,R=0.980.3.PGI is 0. 87 ng/ml,PGII. The minimum detection limit is 0. 46 ng/ml.4.PGI,. PGII has a good linearity in the linear range provided by the kit (R0.99). The serum PGI concentration and PGI/PGII ratio in patients with atrophic gastritis and gastric cancer were significantly lower than those in patients with superficial gastritis (P. 0. 05). With PGI70ng/ml and PGR3 as diagnostic threshold, the sensitivity and specificity of screening gastric cancer were 42.1% and 95.6% respectively. The flow fluorescence method can realize the parallel test of PGI,PGII. The method has good performance and is easy to operate, and it can meet the needs of clinical laboratory. 2. 2. The determination of serum PGI and PGII by flow fluorescence can provide laboratory basis for screening and diagnosis of gastric cancer and atrophic gastritis.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.6
【參考文獻(xiàn)】
相關(guān)期刊論文 前2條
1 肖志堅(jiān),蔣孟軍,肖華龍,呂國(guó)強(qiáng),程兆明,王博誠(chéng);胃癌患者全胃切除后血清PGⅠ、PGⅡ含量變化與胃癌復(fù)發(fā)的關(guān)系[J];癌癥;2000年01期
2 雷勇;;胃蛋白酶原亞群測(cè)定對(duì)胃癌早期診斷預(yù)測(cè)的研究[J];中華實(shí)用診斷與治療雜志;2009年05期
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