【摘要】：目的探討生化試驗(yàn)Carba NP法用于產(chǎn)碳青霉烯酶腸桿菌屬和假單胞菌屬細(xì)菌的快速檢測(cè),以及鑒別區(qū)分產(chǎn)碳青霉烯酶A、B、D類(lèi)型的準(zhǔn)確程度與適用性。方法對(duì)2014年10月-2015年3月從醫(yī)院臨床標(biāo)本分離的23株亞胺培南耐藥菌株進(jìn)行Carba NP法試驗(yàn),并將陽(yáng)性結(jié)果與PCR結(jié)果進(jìn)行比對(duì)。結(jié)果 Carba NP法檢測(cè)的23株胺培南耐藥菌株中4株顯色結(jié)果呈陽(yáng)性,說(shuō)明其產(chǎn)生碳青霉烯酶,并可將其進(jìn)一步區(qū)分為2株A型、2株B型;對(duì)該4株耐藥菌進(jìn)行PCR檢測(cè)產(chǎn)碳青霉烯酶基因,1株含基因VIM,1株含基因NDM(B型),2株含基因KPC(A型),該結(jié)果與Carba NP法結(jié)果相符。結(jié)論 Carba NP法簡(jiǎn)單易行、成本低廉、快速方便,特異性和靈敏度均較高,不僅能快速檢測(cè)出產(chǎn)碳青霉烯酶菌株,并且能進(jìn)一步判定產(chǎn)碳青霉烯酶菌株的類(lèi)型,節(jié)省了檢測(cè)時(shí)間,有助于提高產(chǎn)碳青霉烯酶菌株的檢出率,結(jié)合PCR及測(cè)序能有效控制醫(yī)院獲得性感染。
[Abstract]:Objective to study the rapid detection of carbapenem producing Enterobacter spp and Pseudomonas by biochemical test Carba NP, and to identify the accuracy and applicability of the identification and differentiation of carbapeninase Acarbendase D type. Methods from October 2014 to March 2015, 23 strains of imipenem resistant strains were tested by Carba NP, and the positive results were compared with the results of PCR. Results four of the 23 amponem-resistant strains detected by Carba NP showed positive staining, which indicated that they produced carbapenem, and could be further divided into two strains of type A and two strains of type B. The four strains were tested for carbapenase gene production by PCR, one strain containing gene VIM,1 strain containing gene NDM (B type, and two strains containing gene KPC (A type). The results were consistent with Carba NP method. Conclusion Carba NP method is simple, cheap, rapid, convenient, specific and sensitive. It can not only detect carbapenem producing strains quickly, but also further determine the types of carbapenem producing strains, thus saving the detection time. It is helpful to improve the detection rate of carbapenem producing strains. Combined with PCR and sequencing, nosocomial infection can be effectively controlled.
【作者單位】： 天津市第三中心醫(yī)院檢驗(yàn)科;
【基金】：天津市衛(wèi)生局科技基金資助項(xiàng)目(2013KY02)
【分類(lèi)號(hào)】：R446.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張鞠玲;鮑春梅;陳素明;郭桐生;崔恩博;王歡;張成龍;曲芬;毛遠(yuǎn)麗;;對(duì)亞胺培南不敏感菌株的產(chǎn)碳青霉烯酶的情況分析[J];中國(guó)衛(wèi)生檢驗(yàn)雜志;2011年06期

2 谷秀梅;楊敏;劉文恩;;產(chǎn)碳青霉烯酶菌株實(shí)驗(yàn)室檢測(cè)研究進(jìn)展[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2013年01期

3 王輝,陳民鈞;碳青霉烯酶:未來(lái)困擾我們的難題[J];中華內(nèi)科雜志;2003年05期

4 駱俊;吳衛(wèi)紅;徐曉剛;林東f ;朱德妹;汪復(fù);;耐亞胺培南革蘭陰性桿菌產(chǎn)碳青霉烯酶研究[J];中華檢驗(yàn)醫(yī)學(xué)雜志;2007年05期

5 張致平;碳青霉烯與青霉烯類(lèi)抗生素研究開(kāi)發(fā)的進(jìn)展[J];中國(guó)藥師;1998年02期

6 黃永建;周華;舒賽男;陳中舉;汪s，

本文編號(hào)：2382818