Carba NP法對產碳青霉烯酶菌株快速檢測的研究
發(fā)布時間：2018-12-16 18:13
【摘要】：目的探討生化試驗Carba NP法用于產碳青霉烯酶腸桿菌屬和假單胞菌屬細菌的快速檢測,以及鑒別區(qū)分產碳青霉烯酶A、B、D類型的準確程度與適用性。方法對2014年10月-2015年3月從醫(yī)院臨床標本分離的23株亞胺培南耐藥菌株進行Carba NP法試驗,并將陽性結果與PCR結果進行比對。結果 Carba NP法檢測的23株胺培南耐藥菌株中4株顯色結果呈陽性,說明其產生碳青霉烯酶,并可將其進一步區(qū)分為2株A型、2株B型;對該4株耐藥菌進行PCR檢測產碳青霉烯酶基因,1株含基因VIM,1株含基因NDM(B型),2株含基因KPC(A型),該結果與Carba NP法結果相符。結論 Carba NP法簡單易行、成本低廉、快速方便,特異性和靈敏度均較高,不僅能快速檢測出產碳青霉烯酶菌株,并且能進一步判定產碳青霉烯酶菌株的類型,節(jié)省了檢測時間,有助于提高產碳青霉烯酶菌株的檢出率,結合PCR及測序能有效控制醫(yī)院獲得性感染。
[Abstract]:Objective to study the rapid detection of carbapenem producing Enterobacter spp and Pseudomonas by biochemical test Carba NP, and to identify the accuracy and applicability of the identification and differentiation of carbapeninase Acarbendase D type. Methods from October 2014 to March 2015, 23 strains of imipenem resistant strains were tested by Carba NP, and the positive results were compared with the results of PCR. Results four of the 23 amponem-resistant strains detected by Carba NP showed positive staining, which indicated that they produced carbapenem, and could be further divided into two strains of type A and two strains of type B. The four strains were tested for carbapenase gene production by PCR, one strain containing gene VIM,1 strain containing gene NDM (B type, and two strains containing gene KPC (A type). The results were consistent with Carba NP method. Conclusion Carba NP method is simple, cheap, rapid, convenient, specific and sensitive. It can not only detect carbapenem producing strains quickly, but also further determine the types of carbapenem producing strains, thus saving the detection time. It is helpful to improve the detection rate of carbapenem producing strains. Combined with PCR and sequencing, nosocomial infection can be effectively controlled.
【作者單位】： 天津市第三中心醫(yī)院檢驗科;
【基金】：天津市衛(wèi)生局科技基金資助項目(2013KY02)
【分類號】：R446.5
[Abstract]:Objective to study the rapid detection of carbapenem producing Enterobacter spp and Pseudomonas by biochemical test Carba NP, and to identify the accuracy and applicability of the identification and differentiation of carbapeninase Acarbendase D type. Methods from October 2014 to March 2015, 23 strains of imipenem resistant strains were tested by Carba NP, and the positive results were compared with the results of PCR. Results four of the 23 amponem-resistant strains detected by Carba NP showed positive staining, which indicated that they produced carbapenem, and could be further divided into two strains of type A and two strains of type B. The four strains were tested for carbapenase gene production by PCR, one strain containing gene VIM,1 strain containing gene NDM (B type, and two strains containing gene KPC (A type). The results were consistent with Carba NP method. Conclusion Carba NP method is simple, cheap, rapid, convenient, specific and sensitive. It can not only detect carbapenem producing strains quickly, but also further determine the types of carbapenem producing strains, thus saving the detection time. It is helpful to improve the detection rate of carbapenem producing strains. Combined with PCR and sequencing, nosocomial infection can be effectively controlled.
【作者單位】： 天津市第三中心醫(yī)院檢驗科;
【基金】：天津市衛(wèi)生局科技基金資助項目(2013KY02)
【分類號】：R446.5
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