白城地區(qū)動(dòng)物源鏈球菌耐藥性檢測(cè)及優(yōu)化臨床用藥方案的研究
發(fā)布時(shí)間：2018-11-24 12:51
【摘要】：鏈球菌是引起人畜共患病的主要病原之一,常可引起豬腦膜炎、敗血癥,犢牛菌血癥、心內(nèi)膜炎,奶牛乳房炎,羊急性或慢性呼吸道傳染病等疾病。鏈球菌引發(fā)的疾病主要依賴抗生素的治療,但近年來鏈球菌逐漸出現(xiàn)了耐藥株,因耐藥造成的抗感染失敗屢見不鮮。為此,本研究對(duì)吉林省白城地區(qū)養(yǎng)殖場(chǎng)內(nèi)患有仔豬腦膜炎、敗血癥,奶牛乳房炎的病畜進(jìn)行了病原菌的分離鑒定,并對(duì)其分離獲得的鏈球菌進(jìn)行耐藥性檢測(cè)、耐藥基因檢測(cè)并篩選能減少鏈球菌耐藥突變選擇窗的中西藥合劑,以期優(yōu)化臨床用藥方案,減少因耐藥菌產(chǎn)生造成的經(jīng)濟(jì)損失。本試驗(yàn)從我省白城地區(qū)的患病豬場(chǎng)、牛場(chǎng)采集病料121頭份,通過選擇鑒別培養(yǎng)基、革蘭氏染色、分子生物學(xué)方法對(duì)分離菌株進(jìn)行鑒定,獲得鏈球菌33株。采用瓊脂二倍稀釋法對(duì)分離菌株進(jìn)行耐藥性檢測(cè),結(jié)果顯示,分離菌株均為多重耐藥株,對(duì)6類常見抗菌藥物(β-內(nèi)酰胺類、氟喹諾酮類、四環(huán)素類、氯霉素類、磺胺類、大環(huán)內(nèi)酯類)呈不同程度的交叉耐藥,12%的分離菌對(duì)以上6類抗菌藥物均耐藥,61%以上對(duì)其中4類或5類藥物耐藥,而對(duì)強(qiáng)力霉素和氟苯尼考的交叉耐藥率最低,為24.2%。對(duì)33株動(dòng)物源鏈球菌進(jìn)行了29個(gè)主要耐藥基因的檢測(cè),其中P8、P9株攜帶耐藥基因最多;P20、C2株攜帶耐藥基因最少;57.1%的菌株攜帶7個(gè)以上的耐藥基因。.采用平皿二倍稀釋法測(cè)分離菌防耐藥突變濃度(MPC),確定分離菌株對(duì)抗菌藥物的耐藥突變選擇窗(MSW),并測(cè)定鏈球菌對(duì)瓜蔞、佩蘭醇提物的最小抑菌濃度(MIC),采用棋盤法測(cè)定其聯(lián)合抑菌指數(shù)(FIC),結(jié)果表明瓜蔞醇提物與環(huán)丙沙星,瓜蔞醇提物與氟苯尼考聯(lián)合抑制鏈球菌呈現(xiàn)相加作用;佩蘭醇提物與環(huán)丙沙星聯(lián)合抑制鏈球菌呈現(xiàn)協(xié)同作用;佩蘭醇提物與強(qiáng)力霉素聯(lián)合抑制鏈球菌呈現(xiàn)相加作用,采用微量稀釋法進(jìn)而確定MSW。其結(jié)果顯示,中西藥聯(lián)合可以縮小鏈球菌對(duì)單藥的MSW,能夠有效抑制耐藥菌株的出現(xiàn)。
[Abstract]:Streptococcus is one of the main pathogens of zoonosis, which can cause swine meningitis, septicemia, calves bacteremia, endocarditis, cow mastitis, sheep acute or chronic respiratory infectious diseases and so on. The disease caused by streptococcus mainly depends on the treatment of antibiotics, but in recent years, streptococcus has gradually appeared drug-resistant strains, because of drug resistance caused by the failure of anti-infection is common. Therefore, in this study, the pathogenic bacteria were isolated and identified from piglet meningitis, septicemia and cow mastitis in the breeding farm of Baicheng, Jilin Province, and the drug resistance of streptococcus isolated from them was detected. In order to optimize the clinical drug use plan and reduce the economic loss caused by drug-resistant bacteria, the drug resistance gene detection and screening can reduce the mutation window of streptococcus drug resistance in Chinese and western medicine mixture. In this experiment, 121 samples of diseased materials were collected from the diseased pig farm in Baicheng area of our province. 33 strains of streptococcus were obtained by selecting identification medium, Gram staining and molecular biology method. Agar dilution method was used to detect the drug resistance of the isolated strains. The results showed that the isolates were multidrug resistant, and were resistant to 6 common antibiotics (尾 -lactam, fluoroquinolones, tetracyclines, chloramphenicol, sulfonamides). Among them, 12% isolates were all resistant to the above 6 kinds of antimicrobial agents, and more than 61% were resistant to 4 or 5 of them, while the cross resistance rates to doxycycline and florfenicol were the lowest. For 24.2. Thirty three strains of Streptococcus from animals were tested for drug resistance genes. Among them, P8 P9 strain carried the most drug resistance genes, P20C 2 strain carried the least drug resistance genes, and 57.1% strains carried more than 7 drug resistance genes. Double dilution method was used to determine the antimicrobial resistance mutagenesis window (MSW),) and the minimal inhibitory concentration (MIC),) of Streptococcus to Trichosanthes and Peracetamol extracts (MIC),). The combined bacteriostatic index (FIC),) was determined by chessboard method. The results showed that the alcohol extract of Trichosanthes and ciprofloxacin, the alcohol extract of Trichosanthes and florfenicol combined with florfenicol could inhibit streptococcus. The synergistic effect of perlain alcohol extract and ciprofloxacin on the inhibition of streptococcus was observed, and the synergistic effect of perlain alcohol extract and doxycycline on the inhibition of streptococcus was found. The microdilution method was used to determine MSW.. The results showed that the combination of Chinese and western medicine could effectively inhibit the emergence of drug-resistant strains by reducing the MSW, of streptococcus to a single drug.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.5
本文編號(hào)：2353782
[Abstract]:Streptococcus is one of the main pathogens of zoonosis, which can cause swine meningitis, septicemia, calves bacteremia, endocarditis, cow mastitis, sheep acute or chronic respiratory infectious diseases and so on. The disease caused by streptococcus mainly depends on the treatment of antibiotics, but in recent years, streptococcus has gradually appeared drug-resistant strains, because of drug resistance caused by the failure of anti-infection is common. Therefore, in this study, the pathogenic bacteria were isolated and identified from piglet meningitis, septicemia and cow mastitis in the breeding farm of Baicheng, Jilin Province, and the drug resistance of streptococcus isolated from them was detected. In order to optimize the clinical drug use plan and reduce the economic loss caused by drug-resistant bacteria, the drug resistance gene detection and screening can reduce the mutation window of streptococcus drug resistance in Chinese and western medicine mixture. In this experiment, 121 samples of diseased materials were collected from the diseased pig farm in Baicheng area of our province. 33 strains of streptococcus were obtained by selecting identification medium, Gram staining and molecular biology method. Agar dilution method was used to detect the drug resistance of the isolated strains. The results showed that the isolates were multidrug resistant, and were resistant to 6 common antibiotics (尾 -lactam, fluoroquinolones, tetracyclines, chloramphenicol, sulfonamides). Among them, 12% isolates were all resistant to the above 6 kinds of antimicrobial agents, and more than 61% were resistant to 4 or 5 of them, while the cross resistance rates to doxycycline and florfenicol were the lowest. For 24.2. Thirty three strains of Streptococcus from animals were tested for drug resistance genes. Among them, P8 P9 strain carried the most drug resistance genes, P20C 2 strain carried the least drug resistance genes, and 57.1% strains carried more than 7 drug resistance genes. Double dilution method was used to determine the antimicrobial resistance mutagenesis window (MSW),) and the minimal inhibitory concentration (MIC),) of Streptococcus to Trichosanthes and Peracetamol extracts (MIC),). The combined bacteriostatic index (FIC),) was determined by chessboard method. The results showed that the alcohol extract of Trichosanthes and ciprofloxacin, the alcohol extract of Trichosanthes and florfenicol combined with florfenicol could inhibit streptococcus. The synergistic effect of perlain alcohol extract and ciprofloxacin on the inhibition of streptococcus was observed, and the synergistic effect of perlain alcohol extract and doxycycline on the inhibition of streptococcus was found. The microdilution method was used to determine MSW.. The results showed that the combination of Chinese and western medicine could effectively inhibit the emergence of drug-resistant strains by reducing the MSW, of streptococcus to a single drug.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.5
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 芮萍;馬增軍;段玲欣;劉謝榮;倪靜;沈建忠;;豬鏈球菌對(duì)氟喹諾酮類藥物的耐藥性與靶位突變相關(guān)性[J];畜牧獸醫(yī)學(xué)報(bào);2011年04期
，本文編號(hào)：2353782
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