【摘要】：免疫膠體金試紙檢測(cè)已成為一種常規(guī)的檢測(cè)方法,在生物醫(yī)學(xué)、農(nóng)牧業(yè)、環(huán)境監(jiān)測(cè)、食品安全等方面都有非常廣泛的應(yīng)用。目前試紙檢測(cè)結(jié)果的讀取還處于依賴人眼目測(cè)的定性水平,無(wú)法實(shí)現(xiàn)準(zhǔn)確的定量測(cè)量;此外,一些特殊檢測(cè)需要結(jié)果實(shí)時(shí)留存,例如毒品檢測(cè)等,不僅需要準(zhǔn)確的結(jié)果,還需要提高檢測(cè)效率。因此設(shè)計(jì)一款能夠?qū)崿F(xiàn)快速準(zhǔn)確檢測(cè)、可實(shí)時(shí)記錄存儲(chǔ)、兼容各類膠體金試紙的小型化便攜分析儀成為市場(chǎng)需求。為解決試紙檢測(cè)目前存在的部分問(wèn)題,本論文設(shè)計(jì)了一款基于樹莓派的免疫膠體金試紙檢測(cè)儀。論文主要工作如下:(1)分析膠體金試紙的工作原理,結(jié)合國(guó)內(nèi)外研究現(xiàn)狀及設(shè)計(jì)理念,針對(duì)試紙的反應(yīng)顯色特性設(shè)計(jì)適宜的檢測(cè)方案。(2)完成系統(tǒng)整體結(jié)構(gòu)的搭建,介紹系統(tǒng)檢測(cè)原理,并分別闡述了各個(gè)模塊及其功能,主要包括:均勻照明光路、中央控制系統(tǒng)樹莓派、攝像頭、打印機(jī)和觸摸屏。(3)介紹系統(tǒng)識(shí)別圖像處理圖像的具體方法。系統(tǒng)引入二維碼對(duì)不同類型試紙進(jìn)行區(qū)分,使用二維碼識(shí)別不僅能實(shí)現(xiàn)試紙檢測(cè)區(qū)定位,還能夠擴(kuò)大檢測(cè)儀使用范圍,使儀器實(shí)現(xiàn)多種試紙通用性。檢測(cè)系統(tǒng)將采集到的試紙圖像進(jìn)行灰度化、濾波除噪、圖像分割、二值化等操作,最終成功提取試紙顯色帶的灰度值數(shù)據(jù)。(4)通過(guò)調(diào)整LED工作電壓與攝像頭采集幀率進(jìn)行實(shí)驗(yàn),選擇系統(tǒng)最佳實(shí)驗(yàn)條件。利用一組灰白標(biāo)準(zhǔn)色帶對(duì)試紙的測(cè)量數(shù)據(jù)進(jìn)行修正,使測(cè)量結(jié)果更接近于真實(shí)值。另外重復(fù)性測(cè)量表明系統(tǒng)具有較高的穩(wěn)定性,變異系數(shù)(CV)控制在5‰以內(nèi)。本論文設(shè)計(jì)的免疫膠體金試紙檢測(cè)儀能不但夠準(zhǔn)確測(cè)量出試紙灰度值,而且可實(shí)時(shí)打印檢測(cè)結(jié)果,操作簡(jiǎn)單方便,能夠滿足不同類型試紙的檢測(cè)需求,具有良好的應(yīng)用價(jià)值和發(fā)展前景。
[Abstract]:Immunocolloidal gold test paper has become a conventional detection method, which has been widely used in biomedicine, agriculture and animal husbandry, environmental monitoring, food safety and so on. At present, the reading of test paper test results is still in the qualitative level that depends on human eye measurement, so it is impossible to realize accurate quantitative measurement. In addition, some special tests need real-time retention of results, such as drug detection, not only need accurate results, but also need to improve detection efficiency. Therefore, it is a market demand to design a compact portable analyzer which can be used for fast and accurate detection, real-time recording and storage, and compatible with all kinds of colloidal gold test paper. In order to solve some problems existing in paper test, an immune colloidal gold paper tester based on raspberry pie was designed in this paper. The main work of this paper is as follows: (1) analyzing the working principle of colloidal gold test paper, combining the domestic and foreign research status and design idea, designing the suitable detection scheme for the reaction color characteristic of the test paper. (2) completing the construction of the whole system structure. This paper introduces the principle of system detection, and describes each module and its functions, including: uniform lighting, central control system, raspberry pie, camera, etc. Printer and touch screen. (3) the method of image processing is introduced. The system introduces the two-dimensional code to distinguish different types of test paper. Using the two-dimension code recognition can not only realize the location of test paper detection area, but also expand the scope of use of the tester, so that the instrument can realize the versatility of various test papers. The detection system performs grayscale, filtering and denoising, image segmentation, binarization and so on. Finally, the gray value data of the test paper color display band are extracted successfully. (4) by adjusting the working voltage of LED and the frame rate collected by the camera, the optimal experimental conditions of the system are selected. A group of gray and white ribbons are used to correct the measured data of the test paper, and the results are closer to the real values. In addition, the repeatability measurement showed that the system had high stability, and the coefficient of variation (CV) was controlled within 5 鈥，

本文編號(hào)：2305674