【摘要】：免疫膠體金試紙檢測已成為一種常規(guī)的檢測方法,在生物醫(yī)學、農(nóng)牧業(yè)、環(huán)境監(jiān)測、食品安全等方面都有非常廣泛的應用。目前試紙檢測結果的讀取還處于依賴人眼目測的定性水平,無法實現(xiàn)準確的定量測量;此外,一些特殊檢測需要結果實時留存,例如毒品檢測等,不僅需要準確的結果,還需要提高檢測效率。因此設計一款能夠?qū)崿F(xiàn)快速準確檢測、可實時記錄存儲、兼容各類膠體金試紙的小型化便攜分析儀成為市場需求。為解決試紙檢測目前存在的部分問題,本論文設計了一款基于樹莓派的免疫膠體金試紙檢測儀。論文主要工作如下:(1)分析膠體金試紙的工作原理,結合國內(nèi)外研究現(xiàn)狀及設計理念,針對試紙的反應顯色特性設計適宜的檢測方案。(2)完成系統(tǒng)整體結構的搭建,介紹系統(tǒng)檢測原理,并分別闡述了各個模塊及其功能,主要包括:均勻照明光路、中央控制系統(tǒng)樹莓派、攝像頭、打印機和觸摸屏。(3)介紹系統(tǒng)識別圖像處理圖像的具體方法。系統(tǒng)引入二維碼對不同類型試紙進行區(qū)分,使用二維碼識別不僅能實現(xiàn)試紙檢測區(qū)定位,還能夠擴大檢測儀使用范圍,使儀器實現(xiàn)多種試紙通用性。檢測系統(tǒng)將采集到的試紙圖像進行灰度化、濾波除噪、圖像分割、二值化等操作,最終成功提取試紙顯色帶的灰度值數(shù)據(jù)。(4)通過調(diào)整LED工作電壓與攝像頭采集幀率進行實驗,選擇系統(tǒng)最佳實驗條件。利用一組灰白標準色帶對試紙的測量數(shù)據(jù)進行修正,使測量結果更接近于真實值。另外重復性測量表明系統(tǒng)具有較高的穩(wěn)定性,變異系數(shù)(CV)控制在5‰以內(nèi)。本論文設計的免疫膠體金試紙檢測儀能不但夠準確測量出試紙灰度值,而且可實時打印檢測結果,操作簡單方便,能夠滿足不同類型試紙的檢測需求,具有良好的應用價值和發(fā)展前景。
[Abstract]:Immunocolloidal gold test paper has become a conventional detection method, which has been widely used in biomedicine, agriculture and animal husbandry, environmental monitoring, food safety and so on. At present, the reading of test paper test results is still in the qualitative level that depends on human eye measurement, so it is impossible to realize accurate quantitative measurement. In addition, some special tests need real-time retention of results, such as drug detection, not only need accurate results, but also need to improve detection efficiency. Therefore, it is a market demand to design a compact portable analyzer which can be used for fast and accurate detection, real-time recording and storage, and compatible with all kinds of colloidal gold test paper. In order to solve some problems existing in paper test, an immune colloidal gold paper tester based on raspberry pie was designed in this paper. The main work of this paper is as follows: (1) analyzing the working principle of colloidal gold test paper, combining the domestic and foreign research status and design idea, designing the suitable detection scheme for the reaction color characteristic of the test paper. (2) completing the construction of the whole system structure. This paper introduces the principle of system detection, and describes each module and its functions, including: uniform lighting, central control system, raspberry pie, camera, etc. Printer and touch screen. (3) the method of image processing is introduced. The system introduces the two-dimensional code to distinguish different types of test paper. Using the two-dimension code recognition can not only realize the location of test paper detection area, but also expand the scope of use of the tester, so that the instrument can realize the versatility of various test papers. The detection system performs grayscale, filtering and denoising, image segmentation, binarization and so on. Finally, the gray value data of the test paper color display band are extracted successfully. (4) by adjusting the working voltage of LED and the frame rate collected by the camera, the optimal experimental conditions of the system are selected. A group of gray and white ribbons are used to correct the measured data of the test paper, and the results are closer to the real values. In addition, the repeatability measurement showed that the system had high stability, and the coefficient of variation (CV) was controlled within 5 鈥，

本文編號：2305674