【摘要】：目的使用VITEK MS質譜鑒定系統(tǒng)對臨床分離的病原菌進行鑒定,并評價其性能,為病原菌的快速鑒定提供新的方法。方法收集2014年4-7月醫(yī)院臨床分離病原菌共948株,經VITEK-2Compact細菌鑒定儀及16SrRNA、rpoB、hisA、ITS基因片段測序確認,使用VITEK MS基質輔助激光解析電離飛行時間質譜進行鑒定,并對8株真菌連續(xù)檢測測試質譜儀穩(wěn)定性。結果共分離到鏈球菌屬191株、腸球菌屬94株、葡萄球菌屬144株、腸桿菌科細菌193株、非發(fā)酵革蘭陰性菌170株、臨床常見其他菌36株、酵母菌屬102株和絲狀真菌18株。VITEK MS對入選菌株的鑒定總準確率達97.10%,但其對沙門菌屬和鮑氏不動復合群的鑒定僅能到菌屬或復合群水平;對酵母菌屬的準確鑒定率為100.00%,絲狀真菌中除鏈格孢外(數據庫未包含此菌),88.89%的絲狀真菌得到準確鑒定;VITEK MS質譜儀對真菌連續(xù)5d檢測結果一致,穩(wěn)定性良好。結論 VITKE MS表現穩(wěn)定、準確率高、耗時短,尤其在真菌的鑒定上凸顯優(yōu)勢,可作為臨床細菌快速鑒定的手段。
[Abstract]:Objective to identify and evaluate the clinical pathogenic bacteria by VITEK MS mass spectrometry, and to provide a new method for rapid identification of pathogenic bacteria. Methods A total of 948 strains of pathogenic bacteria were collected from hospitals from April to July 2014. The results were confirmed by VITEK-2Compact bacteria identification instrument and 16s rRNA-rpoBnb hisAITS gene fragment sequencing. VITEK MS matrix-assisted laser desorption ionization time of flight mass spectrometry was used to identify 948 strains of pathogenic bacteria. The stability of mass spectrometer was tested by continuous detection of 8 strains of fungi. Results A total of 191 strains of Streptococcus, 94 strains of Enterococcus, 144 strains of Staphylococcus, 193 strains of Enterobacteriaceae, 170 strains of non-fermentative Gram-negative bacteria, 36 strains of common clinical bacteria were isolated. 102 strains of yeast and 18 filamentous fungi. VITEK MS had a total accuracy of 97.1010, but the identification of Salmonella and Baumer's immobility complex could only reach the level of genus or complex. The accurate identification rate of the genus yeast was 100.00g, and 88.89% of filamentous fungi except Alternaria (which was not included in the database) were identified accurately by vitek MS mass spectrometer for 5 consecutive days, and the results were consistent, and the stability of the filamentous fungi was good. Conclusion VITKE MS has the advantages of stable performance, high accuracy, short time consuming, especially in the identification of fungi. It can be used as a method for rapid identification of clinical bacteria.
【作者單位】： 浙江省桐廬縣第一人民醫(yī)院檢驗科;浙江大學醫(yī)學院附屬第二醫(yī)院檢驗科;
【基金】：國家自然科學基金資助項目(81401720)
【分類號】：R446.5
，

本文編號：2253046