【摘要】：目的:為確認(rèn)新型過敏原特異性Ig E抗體檢測(cè)試劑盒(芯片法)的可信度,將芯片法與熒光酶聯(lián)免疫法進(jìn)行對(duì)比分析。方法:芯片法運(yùn)用熒光免疫吸附分析的原理,針對(duì)6種過敏原的特異性Ig E進(jìn)行檢測(cè),并對(duì)比與熒光酶聯(lián)免疫法檢測(cè)結(jié)果的陽性一致率、陰性一致率、整體一致率,探討kappa值。共有456例樣本用于對(duì)比分析。結(jié)果:芯片法所檢測(cè)的陽性率分別為屋塵螨88.6%、貓毛皮屑92.9%、雞蛋白92.5%、蝦91.5%、蚌99.3%、蟹92.2%;而熒光酶聯(lián)免疫法的陽性率分別為屋塵螨88.6%、貓毛皮屑92.9%、雞蛋白92.5%、蝦85.2%、蚌93.1%、蟹92.2%。6種過敏原的kappa值分別為屋塵螨1.000、貓皮屑1.000、雞蛋白1.000、蝦0.695、蚌0.172與蟹1.000。對(duì)于4種常見過敏原屋塵螨、貓皮屑、雞蛋白與蟹,芯片法與熒光酶聯(lián)免疫法的測(cè)定結(jié)果完全一致。蝦與蚌的檢測(cè)一致性偏低,可能因?yàn)殛幮詷颖緮?shù)偏低、檢測(cè)值位于閾值附近及芯片法敏感度較高所導(dǎo)致。結(jié)論:芯片法與熒光酶聯(lián)免疫法檢測(cè)變應(yīng)原特異性Ig E具有高度一致性,芯片法的檢測(cè)結(jié)果敏感性高,是臨床上過敏原特異性Ig E檢測(cè)的可靠工具。
[Abstract]:Aim: to confirm the reliability of the new allergen specific Ig E antibody detection kit (chip method), the chip assay and the fluorescence enzyme linked immunosorbent assay (FIA) were compared and analyzed. Methods: the principle of fluorescence immunosorbent assay (FIA) was used to detect the specific Ig E of 6 allergens. The positive, negative and overall kappa values were compared with the results of fluorescence enzyme-linked immunosorbent assay (FIA). A total of 456 samples were used for comparative analysis. Results: the positive rates of the microarray method were 88.6m, 92.9cm, 92.5, 91.5, 99.3and 92.2respectively, while the positive rates of fluorescence enzyme-linked immunosorbent assay were 88.6m for house dust mite, 92.9for cat hair dander, 92.5 for egg white, 85.2for shrimp, 93.1for mussel, 92.2for crab. The kappa values of allergen were 1.000 for house dust mite, 1.000 for cat dander, 1.000 for egg white, 0.695 for shrimp, 0.172 for mussel and 1.000for crab. For four common allergen house dust mites, cat dandruff, egg white and crab, the results of microarray and fluorescence enzyme linked immunosorbent assay were in good agreement. The low consistency between shrimp and clam may be due to the low number of negative samples, the detection value located near the threshold value and the high sensitivity of microarray method. Conclusion: the detection of allergen-specific Ig E by microarray and fluorescence enzyme-linked immunosorbent assay is highly consistent, and the sensitivity of microarray assay is high. It is a reliable tool for the detection of allergen specific Ig E in clinic.
【作者單位】： 北京大學(xué)第三醫(yī)院皮膚科;
【分類號(hào)】：R446.6
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本文編號(hào)：2218396