本文選題：結(jié)核病 + 潛伏感染　； 參考：《中國(guó)病原生物學(xué)雜志》2016年04期

【摘要】：目的探討7種細(xì)胞因子的mRNA表達(dá)水平作為診斷結(jié)核分枝桿菌潛伏感染生物標(biāo)志物的可能性。方法健康對(duì)照64人,活動(dòng)性肺結(jié)核50人,結(jié)核分枝桿菌潛伏感染60人,采用實(shí)時(shí)熒光定量PCR(qRT-PCR)檢測(cè)結(jié)核特異性抗原刺激的外周血中TNF-α、IFN-γ、IL-2、IL-10、IFI35、CXCL10及Foxp3的mRNA表達(dá)水平。結(jié)果 7種因子相對(duì)表達(dá)量在活動(dòng)性肺結(jié)核組分別為2.33±0.09、4.94±0.45、150±17.82、2.02±0.55、9.53±5.96、5.56±0.97、1.24±0.17,潛伏感染組分別為3.2±0.61、10.04±1.89、202±17.6、21.89±10.25、29.17±11.19、41.35±11.46、2.14±0.67,健康組分別為1.2±0.08、2.92±0.03、53±17.82、1.48±0.17、1.28±0.77、1.63±0.25、1.03±0.67。潛伏感染組與健康組相比7種細(xì)胞因子的mRNA表達(dá)水平,差異均有統(tǒng)計(jì)學(xué)意義(P0.01),相應(yīng)細(xì)胞因子檢測(cè)的敏感性、特異性分別為95.1%、82.5%,92.5%、88.7%,91.9%、90.1%,88.2%、51.5%,74.2%、61.2%,81.8%、73.4%和80.4%、51.3%;潛伏感染組和活動(dòng)性結(jié)核組比較除IFI35因子外,其余6種細(xì)胞因子的mRNA表達(dá)水平差異均有統(tǒng)計(jì)學(xué)意義(P0.05),相應(yīng)細(xì)胞因子檢測(cè)的特異性、敏感性分別為90.6%、80%,88.6%、92.7%,91.9%、86.2%,57.6%、60%,50%、58%,76%、72.2%和66.7%、67.1%;健康對(duì)照組和活動(dòng)性結(jié)核組相比較,7種細(xì)胞因子的mRNA表達(dá)水平差異均有統(tǒng)計(jì)學(xué)意義(P0.01),相應(yīng)細(xì)胞因子檢測(cè)的敏感性、特異性分別為92.3%、90%,88.6%、86.7%,94.4%、86.7%,89.7%、65.8%,82.4%、63.3%,88.2%、70%和92.9%、72.1%。結(jié)核病密切接觸者潛伏感染率為48.97%,健康組潛伏感染率為36%。結(jié)論結(jié)核病患者血清TNF-α、IFN-γ、IL-2mRNA水平升高,以潛伏感染者升高更顯著,因此可作為診斷結(jié)核分枝桿菌潛伏感染的生物標(biāo)志物。
[Abstract]:Objective to explore the possibility of mRNA expression of seven cytokines as biomarkers for the diagnosis of latent infection of Mycobacterium tuberculosis. Methods A total of 64 healthy controls, 50 active pulmonary tuberculosis patients and 60 patients with latent infection of Mycobacterium tuberculosis were used to detect the mRNA expression of TNF- 偽, IFN- 緯, IL-2IL-10, IL-10, IFI35, CXCL10 and Foxp3 mRNA in peripheral blood stimulated by specific antigen of tuberculosis (QRT-PCR). Results the relative expression levels of seven factors in active pulmonary tuberculosis group were 2.33 鹵0.09 鹵4.94 鹵0.45150 鹵17.82 鹵2.02 鹵0.559.53 鹵5.965.56 鹵0.971.24 鹵0.17, respectively, and those in latent infection group were 3.2 鹵0.61n 10.04 鹵1.89202 鹵17.629.17 鹵10.2529.17 鹵11.1929.17 鹵11.466.2.14 鹵0.67and 1.2 鹵0.082.92 鹵0.0353 鹵17.821.48 鹵0.171.28 鹵0.771.63 鹵0.251.03 respectively. The mRNA expression levels of 7 cytokines in latent infection group were significantly higher than those in healthy group (P0.01). The sensitivity and specificity of the corresponding cytokine detection were 95.1, 82.5 and 92.5, respectively. The sensitivity of the corresponding cytokines in the latent infection group was 73.4% and 80.4% and 51.3%, respectively. The sensitivity of the corresponding cytokines was 95.1% and 82.5%, respectively. The sensitivity of the corresponding cytokines in the latent infection group was higher than that in the active group, and the specificity was 91.9%. The sensitivity of the corresponding cytokines in the latent infection group was 73.4% and 80.4% in the active group, except IFI35 in the latent infection group and the active group, in addition to IFI35, the difference between the latent infection group and the active group was significant. The mRNA expression levels of the other six cytokines were significantly different (P0.05). 鏁忔劅鎬у垎鍒負(fù)90.6%,80%,88.6%,92.7%,91.9%,86.2%,57.6%,60%,50%,58%,76%,72.2%鍜，

本文編號(hào)：2105709