本文選題：洞穴 + 放線菌　； 參考：《中國(guó)抗生素雜志》2016年03期

【摘要】：目的勘探湖北利川喀斯特洞穴放線菌多樣性并進(jìn)行抗菌活性篩選,以期發(fā)現(xiàn)新的藥用微生物資源,為新抗生素發(fā)現(xiàn)奠定基礎(chǔ)。方法采用10種分離培養(yǎng)基,從來(lái)自3個(gè)洞穴內(nèi)的38份樣品中分離放線菌;通過(guò)基于16S r RNA基因序列的系統(tǒng)發(fā)育學(xué)分析,開(kāi)展放線菌多樣性研究;對(duì)67株放線菌發(fā)酵液乙酸乙酯萃取濃縮物和菌絲體丙酮浸提濃縮物進(jìn)行抗菌活性篩選;陽(yáng)性菌株進(jìn)行次級(jí)代謝產(chǎn)物生物合成基因PKSⅠ、PKSⅡ和NRPS的初步篩選。結(jié)果從38份樣品中共分離得到197株放線菌,它們分布于6個(gè)目13個(gè)科24個(gè)屬,其中鏈霉菌為優(yōu)勢(shì)菌屬,菌株T3-7-1為微桿菌科的一個(gè)潛在新分類單元;發(fā)酵67株放線菌,均在至少一個(gè)抗菌活性檢測(cè)中顯示為陽(yáng)性;67株陽(yáng)性菌株中有63株基于PKSⅠ、PKSⅡ或NRPS基因的引物PCR擴(kuò)增出相應(yīng)的目的條帶,表明這些放線菌可能含有相應(yīng)的PKSⅠ、PKSⅡ或NRPS基因。結(jié)論湖北利川喀斯特洞穴中蘊(yùn)藏著豐富的放線菌資源,具有從中發(fā)現(xiàn)放線菌新物種及新抗生素的潛力,值得進(jìn)一步研究。
[Abstract]:Objective to explore the diversity of actinomycetes in karst caves of Lichuan County Hubei Province and screen their antibacterial activities in order to find new medicinal microbial resources and lay a foundation for the discovery of new antibiotics. Methods Actinomycetes were isolated from 38 samples from 3 caves on 10 culture medium, and the diversity of actinomycetes was studied by phylogenetic analysis based on 16s r RNA gene sequence. The antimicrobial activity of 67 strains of actinomycetes was screened with ethyl acetate extract concentrate and mycelium acetone extract concentrate, and the secondary metabolite biosynthesis genes PKS 鈪，

本文編號(hào)：1902086