本文選題：喹諾酮 + gyrA基因　； 參考：《中國人獸共患病學報》2016年10期

【摘要】：目的為闡明寧波地區(qū)耐多藥結核分枝桿菌(Multiple drug-resistant tuberculosis,MDR-TB)的gyr基因突變特征,深入研究MDR-TB對喹諾酮類藥物耐藥與gyr基因突變特征的關系。方法采用1%比例法對MDR-TB進行氧氟沙星藥敏檢測實驗,通過DNA直接測序法分析MDR-TB的gyr基因突變情況。結果 120株MDR-TB臨床分離株中有34株對喹諾酮耐藥,總耐藥率為28.33%(34/120)。34株耐喹諾酮菌株中,30株gyr基因發(fā)生突變,突變率為88.24%(30/34)。30株gyr基因發(fā)生突變的菌株中gyrA基因突變有29株,占96.67%(29/30),突變位點包括90、91和94位氨基酸;gyrB基因突變有2株,其中1株均合并gyrA基因突變,占6.67%(2/30),突變位點包括499和502位氨基酸。結論寧波地區(qū)MDRTB對喹諾酮類藥物耐藥形勢較為嚴峻,gyrA基因突變與MDR-TB對喹諾酮類藥物耐藥相關。
[Abstract]:Objective to study the relationship between MDR-TB resistance to quinolones and gyr gene mutation in Ningbo area in order to elucidate the characteristics of gyr gene mutation in multidrug resistant Mycobacterium tuberculosis (MDR-TB). Methods 1% ratio method was used to detect the susceptibility of MDR-TB to ofloxacin and the mutation of gyr gene in MDR-TB was analyzed by DNA direct sequencing. Results among the 120 clinical isolates of MDR-TB, 34 were resistant to quinolones, and the total resistance rate was 28.33%. Among the 34 / 120 / 34 quinolone-resistant strains, 30 gyr genes were mutated, and the mutation rate was 88.24% 30% 34 / 30 gyr gene mutation in 29 strains of gyrA gene. There were two mutations in 90.91 and 94 amino acid gyrB genes, one of which was combined with gyrA gene mutation, which accounted for 6.6770 / 30, and the mutation sites included 499 and 502 amino acids. Conclusion MDRTB resistance to quinolones is more severe in Ningbo area. The mutation of gyra gene is associated with MDR-TB resistance to quinolones.
【作者單位】： 浙江省寧波市疾病預防控制中心結核病防制所;
【基金】：寧波市自然科學基金(No.2015A610195) 浙江省公共衛(wèi)生應急檢測關鍵技術重點實驗室開放基金資助~~
【分類號】：R440
，

本文編號：1881024