本文選題：食用白酒　切入點(diǎn)：血糖　出處：《貴陽(yáng)醫(yī)學(xué)院》2015年碩士論文

【摘要】：目的:對(duì)SD大鼠進(jìn)行灌胃,探討攝入某品牌兩種濃度食用白酒(54°和38°)后,不同時(shí)長(zhǎng)及不同劑量對(duì)大鼠空腹血糖、胰島B細(xì)胞表達(dá)胰島素(Ins)、A細(xì)胞表達(dá)胰高血糖素(Glu)及血清Ins及C肽(C-P)的改變,以期為探索科學(xué)健康飲酒提供一定實(shí)驗(yàn)依據(jù)。方法:正常成年雄性SD大鼠90只,隨機(jī)分為實(shí)驗(yàn)組(experiment group,EG)及正常對(duì)照組(normal control group,NCG)。(1)54°實(shí)驗(yàn)組45只:分為4周組(4w)、8周組(8w)、12周組(12w),其中每周組均分為低劑量組(low dose group,L組,0.8ml/kg·day)、中劑量組(middle dose group,M組,1.6ml/kg·day)、高劑量組(high dose group,H組,2.4ml/kg·day),每個(gè)劑量組5只大鼠。(2)38°實(shí)驗(yàn)組30只:分為4w、12w;各周組又分為三個(gè)劑量組,即L組、M組、H組,每個(gè)劑量組5只大鼠;(3)各周組大鼠均有5只作正常對(duì)照。實(shí)驗(yàn)組大鼠予相應(yīng)食用白酒灌胃,每日11:00Am及5:00Pm各灌胃一次,正常對(duì)照組不予處理。分別于第4w末,第8w末,第12w末測(cè)空腹血糖,取胰尾組織及血清,采用放射免疫法、免疫組化SABC法、圖像分析及形態(tài)計(jì)量法進(jìn)行研究。結(jié)果:1.54°組:(1)各實(shí)驗(yàn)組大鼠空腹血糖水平與正常對(duì)照組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。(2)血清Ins放免結(jié)果顯示:僅8w(H)組、12w(L)組大鼠血清Ins水平與正常對(duì)照組比較有所上升,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),其余各組未見(jiàn)明顯變化。(3)免疫組化結(jié)果顯示:光鏡下,Ins、Glu免疫反應(yīng)陽(yáng)性產(chǎn)物呈棕黃至棕褐色細(xì)顆粒狀,位于胞質(zhì)內(nèi)。①與正常對(duì)照組比較,除8w(H)組和12w(L)組稍有升高外(P0.05),余實(shí)驗(yàn)各組Ins陽(yáng)性細(xì)胞的免疫染色強(qiáng)度及平均光密度的差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。②與正常對(duì)照組比較,除12w(L)組稍有升高(P0.05)外,余實(shí)驗(yàn)各組Glu陽(yáng)性細(xì)胞的免疫染色強(qiáng)度及平均光密度的差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。(4)形態(tài)學(xué)計(jì)量結(jié)果顯示:與正常組比較,各實(shí)驗(yàn)組Ins陽(yáng)性細(xì)胞及Glu陽(yáng)性細(xì)胞的面數(shù)密度(NA)均未見(jiàn)明顯變化(P0.05)。2.38°組:(1)各實(shí)驗(yàn)組大鼠空腹血糖水平與正常對(duì)照組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。(2)血清C肽放免結(jié)果顯示:與正常對(duì)照組比較,僅12w(L)組血清C肽水平稍有升高(P0.05),余各實(shí)驗(yàn)組未見(jiàn)明顯改變。(3)免疫組化結(jié)果顯示:各實(shí)驗(yàn)組Ins陽(yáng)性細(xì)胞、Glu陽(yáng)性細(xì)胞的分布、數(shù)量及免疫染色強(qiáng)度與正常組比較未見(jiàn)明顯變化;①各實(shí)驗(yàn)組Ins陽(yáng)性細(xì)胞、Glu陽(yáng)性細(xì)胞平均光密度值未見(jiàn)明顯改變(P0.05);②與正常組比較,各實(shí)驗(yàn)組Ins陽(yáng)性細(xì)胞、Glu陽(yáng)性細(xì)胞的面數(shù)密度(NA)亦未見(jiàn)明顯改變(P0.05)。結(jié)論:某品牌54°及38°食用白酒灌胃,在本實(shí)驗(yàn)設(shè)定的劑量和時(shí)程內(nèi),大鼠血糖未見(jiàn)明顯異常;對(duì)胰島B細(xì)胞表達(dá)Ins、胰島A細(xì)胞表達(dá)Glu和面數(shù)密度、血清Ins及C肽無(wú)明顯影響。
[Abstract]:Objective: to study the effects of different time and dosage on fasting blood glucose of SD rats after ingesting two different concentrations of liquor (54 擄and 38 擄). In order to provide some experimental evidence for the study of scientific and healthy drinking, the expression of glucagon Glua and serum Ins and C-Pin in islet B cells were determined. Methods: 90 adult male Sprague-Dawley rats were included in this study. The experiment group was randomly divided into two groups: experimental group (n = 45) and normal control group group (n = 45). The experimental group (n = 45) was divided into 4 weeks group (n = 45) and a control group (n = 45). The experimental group was divided into 4 weeks group, 8 weeks group, 8 weeks group and 12 weeks group, respectively. Each week group was divided into low dose group group group (0. 8 ml / kg), middle dose group group (middle dose group M group) and high dose group group (1. 6 ml / kg), and the high dose group group group was divided into two groups: low dose group group (0. 8 ml / kg) and middle dose group group M group (1. 6 ml / kg). The experimental group (n = 30) was divided into 4 weeks and 12 weeks, and each week group was divided into three dose groups. There were 5 rats in each week group as normal control group. Rats in the experimental group were given corresponding drinking liquor, 11:00Am and 5:00Pm were given orally once a day, and the normal control group was not treated at the end of 4 weeks. Fasting blood glucose was measured at the end of the 8th week and the end of the 12th week. The pancreatic tail tissue and serum were collected. Radioimmunoassay and immunohistochemical SABC method were used. Image analysis and morphometry were used to study the results. Results the fasting blood glucose levels of the rats in the 1: 1.54 擄group were compared with those in the normal control group. The results of serum Ins radioimmunoassay showed that the serum Ins level of rats in the group of only 8 weeks of HH) was higher than that in the control group at 12 ws (P < 0.05), and the serum Ins level of the rats in the control group was significantly higher than that in the control group (P < 0.05). The difference was statistically significant (P 0.05), but there was no significant change in the other groups. The results of immunohistochemistry showed that the immunoreactive products of Instrol Glu were brown and brown, and they were located in cytoplasm of 1. 1 as compared with the normal control group. Except for the 8w HG and 12wL) groups, the immunoreactivity and average optical density of the Ins positive cells in the other groups were not significantly different from those in the normal control group, except for the 12 wk L) group, which was slightly higher than that of the control group (P 0.05), and the other two groups had no significant difference in the immunostaining intensity and the average optical density (P 0.05), except for the 12 wk L) group, which had a slight increase (P 0.05). There was no significant difference in the immunostaining intensity and average optical density of Glu positive cells in the remaining experimental groups. The results of morphological measurement showed that: compared with the normal group, there was no significant difference between the two groups. There was no significant change in the number density of Ins positive cells and Glu positive cells in each experimental group. (P0.05U. 2.38 擄group) the fasting blood glucose levels of rats in each experimental group were compared with those of the normal control group. There was no significant difference in serum C-peptide radioimmunoassay results: compared with the normal control group, there was no significant difference in serum C-peptide radioimmunoassay. The level of serum C-peptide was slightly increased in 12 weeks (P < 0.05), but no significant change was found in the other experimental groups. The immunohistochemical results showed that the distribution of Ins positive cells in each experimental group was not obvious, and the distribution of Glu-positive cells in the other experimental groups was higher than that in the control group (P < 0.05). There was no significant change in the number and the intensity of immunostaining compared with the normal group. The average optical density of the Ins positive cells in each experimental group was not significantly changed compared with the normal group, and there was no significant change in the average optical density of the Glu-positive cells in each experimental group compared with the normal group. The surface number density of Ins positive cells in each experimental group was not significantly changed (P 0.05). Conclusion: a brand of 54 擄and 38 擄edible liquor was perfused by stomach, and there was no obvious abnormality of blood glucose in rats during the time course and dose set in this experiment. [WT5HZ] [WT5BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ]. The expression of Ins in islet B cells, Glu expression and surface number density in islet A cells, serum Ins and C-peptide were not significantly affected.
【學(xué)位授予單位】：貴陽(yáng)醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R446.6

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