本文選題：大鼠　切入點(diǎn)：睡眠剝奪　出處：《新鄉(xiāng)醫(yī)學(xué)院》2015年碩士論文

【摘要】：背景現(xiàn)有證據(jù)表明,在睡眠剝奪期間與清醒期間有著不同的基因表達(dá)。睡眠剝奪對(duì)5-HT、DA等神經(jīng)遞質(zhì)的改變已有相關(guān)的研究,但它們之間的平衡轉(zhuǎn)換和協(xié)同作用的調(diào)節(jié)機(jī)制目前尚不清楚。目的通過檢測大鼠在不同時(shí)間睡眠剝奪三個(gè)腦區(qū)(海馬、下丘腦、紋狀體)5-HT受體1A、DA受體D1基因和DA受體D2基因表達(dá)改變情況,分析睡眠剝奪對(duì)這些基因表達(dá)的影響,探討不同神經(jīng)遞質(zhì)通路參與睡眠調(diào)節(jié)的差異。方法1選擇10周齡雄性SD大鼠24只。分別進(jìn)行24小時(shí)、48小時(shí)、72小時(shí)REM睡眠剝奪。2采用曠場實(shí)驗(yàn)檢測動(dòng)物潛伏期、水平分?jǐn)?shù)、直立次數(shù)。驗(yàn)證睡眠剝奪體力和精神疲勞模型。3每組取大鼠海馬、下丘腦、紋狀體三個(gè)腦區(qū)腦組織,利用RT-PCR技術(shù)分別檢測5羥色胺1A受體和多巴胺D1R、D2R基因的表達(dá)情況。4應(yīng)用SPSS11.5統(tǒng)計(jì)軟件對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行相應(yīng)的統(tǒng)計(jì)與處理。計(jì)量資料進(jìn)行統(tǒng)計(jì)分析選用均數(shù)±標(biāo)準(zhǔn)差(x±s)。兩組間均數(shù)進(jìn)行對(duì)比與分析采用獨(dú)立樣本t驗(yàn)；多組間均數(shù)進(jìn)行比較采用單因素方差分析,進(jìn)行兩兩比較采用LSD檢驗(yàn),進(jìn)行相關(guān)性分析采用Pearson相關(guān),P0.05表示統(tǒng)計(jì)結(jié)果的差異具有顯著性。結(jié)果1 SD大鼠經(jīng)過睡眠剝奪后進(jìn)行曠場實(shí)驗(yàn),水平得分及垂直得分均有很大的波動(dòng),大鼠對(duì)環(huán)境的認(rèn)知能力明顯下降,體力、精神疲勞。說明睡眠剝奪對(duì)機(jī)體的精神活動(dòng)產(chǎn)生抑制。2睡眠剝奪影響了大鼠海馬(F=56.203,P=0.000)和紋狀體(F=77.288,P=0.000)區(qū)域5-HT1A的基因表達(dá),差異具有統(tǒng)計(jì)學(xué)意義,在海馬和紋狀體區(qū)域各睡眠剝奪組大鼠的表達(dá)量均高于對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),在海馬剝奪72小時(shí)組高于剝奪24小時(shí)(P=0.005)和48小時(shí)(P=0.010)組,紋狀體區(qū)域則各組動(dòng)物的差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。各組大鼠下丘腦區(qū)域5-HT1A的基因表達(dá)差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。3睡眠剝奪影響了大鼠海馬(F=74.708,P=0.000)、紋狀體(F=80.687,P=0.000)區(qū)域D2基因的表達(dá),差異具有統(tǒng)計(jì)學(xué)意義,各睡眠剝奪組大鼠在海馬和紋狀體區(qū)域的表達(dá)量均低于對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),剝奪時(shí)間長的動(dòng)物基因表達(dá)量也更低,海馬與紋狀體腦區(qū)基因表達(dá)量各組動(dòng)物的差異均具有統(tǒng)計(jì)學(xué)意義(P均0.05)。各組大鼠下丘腦區(qū)域D2R的基因表達(dá)表達(dá)差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。4相關(guān)分析顯示對(duì)照組各腦區(qū)5-HT1A和D2R基因的表達(dá)不存在相關(guān)性,睡眠剝奪24小時(shí)在海馬(r=-0.868,P=0.025)、紋狀體(r=-0.883,P=0.02)、下丘腦(r=-0.841,P=0.036)兩個(gè)基因的表達(dá)均呈負(fù)相關(guān),睡眠剝奪48小時(shí)和72小時(shí)在海馬(r=-0.881,P=0.02；r=-0.878,P=0.021)、紋狀體(r=-0.735,P=0.006；r=-0.855,P=0.03)兩個(gè)基因的表達(dá)也呈負(fù)相關(guān)。5睡眠剝奪對(duì)大鼠海馬、紋狀體和下丘腦區(qū)域D1R基因表達(dá)表達(dá)差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。小結(jié)睡眠剝奪大鼠在海馬和紋狀體5-HT1A與D2R基因表達(dá)均發(fā)生顯著變化,5-HT1A基因表達(dá)增高、D2R基因表達(dá)下降,并呈負(fù)相關(guān),表明5-羥色胺系統(tǒng)和多巴胺系統(tǒng)在受體水平對(duì)睡眠節(jié)律的調(diào)節(jié)具有協(xié)同性。
[Abstract]:The background of the current evidence shows that there is in sleep deprivation during the waking period and different gene expression. Sleep deprivation on 5-HT, on DA related neurotransmitter changes already, but the regulatory mechanism of balance conversion and synergy among them is unclear. Objective to detect rats in sleep deprivation three brain regions (hippocampus, hypothalamus, striatum) 5-HT receptor 1A, DA receptor expression of D1 gene and DA receptor D2 gene changes, analysis of effects of sleep deprivation on the expression of these genes, the differences between different neurotransmitter pathways involved in sleep regulation. Methods: 1 10 week old male SD 24 rats were 24. Hours, 48 hours, 72 hours of sleep deprivation REM.2 using the open field test animal level scores, rearing latency, sleep deprivation. Verify the physical and mental fatigue model.3 from each rat hippocampus, hypothalamus, striatum The brain of three brain regions, the use of RT-PCR technology to detect 5 serotonin 1A receptor and dopamine D1R, D2R gene expression of.4 using SPSS11.5 statistical software for statistical processing and corresponding to the experimental data. The measurement data were used for statistical analysis the mean and standard deviation (x + s) between the two groups were analyzed. Comparison and analysis by using independent sample t test; multiple groups were compared using one-way ANOVA, 22 were compared with LSD test and correlation analysis using Pearson correlation, P0.05 said the difference was significant. The statistical results of 1 SD rats after sleep deprivation after the open field test scores and level fluctuation the vertical score significantly, cognitive ability of rats decreased significantly in the environment, physical and mental fatigue. It inhibited the effect of.2 sleep deprivation rat hippocampus on the mental activity of sleep deprivation (F=56. 203, P=0.000) and striatum (F=77.288, P=0.000) expression region of 5-HT1A gene, there was significant difference in the expression of hippocampus and striatum of the sleep deprivation group rats were higher than the control group, the difference was statistically significant (P0.05) in the hippocampus, 72 hours deprivation group was higher than that of deprivation for 24 hours (P=0.005) and 48 h (P=0.010) group, the difference of each animal striatum were statistically significant (P0.05). Each group the hypothalamus 5-HT1A rat gene expression difference was not statistically significant (P0.05).3 sleep deprivation affects the hippocampus (F=74.708, P=0.000), striatum (F=80.687, P=0.000) expression of D2 gene region, the difference was statistically significant, the sleep deprivation group rats were lower than the control group in the expression of hippocampus and striatum volume, the difference was statistically significant (P0.05), deprived of animal gene expression is lower in long time, The difference of gene expression in hippocampus and striatum area of each animal were statistically significant (P < 0.05). Each region of the hypothalamus of rat D2R gene expression difference was not statistically significant (P0.05).4 correlation analysis showed that 5-HT1A and D2R control group of brain gene expression is associated with sleep deprivation for 24 hours (r=-0.868, P=0.025) in the hippocampus, striatum, hypothalamus (r=-0.883, P=0.02) (r=-0.841, P=0.036) two gene expression was negatively related to sleep deprivation for 48 hours and 72 hours in the hippocampus (r=-0.881, P=0.02; r= -0.878, P=0.021), striatum (r=-0.735, P=0.006; r=-0.855, P=0.03) two the gene was also negatively related to.5 sleep deprivation on rat hippocampus, striatum and hypothalamus D1R gene expression difference was not statistically significant (P0.05). Summary of sleep deprivation rats in the hippocampus and striatum of 5-HT1A and D2R gene All of them had significant changes. The expression of 5-HT1A gene increased, and the expression of D2R gene decreased. It was negatively correlated, indicating that 5- serotonin system and dopamine system play a synergistic role in the regulation of sleep rhythm at the receptor level.

【學(xué)位授予單位】：新鄉(xiāng)醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R740

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