本文選題：鐵蛋白　切入點(diǎn)：時(shí)間分辨熒光免疫分析法　出處：《現(xiàn)代免疫學(xué)》2016年01期

【摘要】：建立鐵蛋白(FER)時(shí)間分辨熒光免疫層析檢測法(TRFIA-POCT)并進(jìn)行臨床應(yīng)用。將FER抗體和兔IgG包被在硝酸纖維素膜(NC膜)上,以熒光微球標(biāo)記抗FER單克隆抗體及兔IgG分別作為檢測線和質(zhì)控線制備熒光免疫層析試紙條。FER-TRFIA-POCT的靈敏度為0.5ng/ml;批內(nèi)CV為3.2%~4.6%,批間CV為3.8%~5.2%;平均回收率為100.5%;熱穩(wěn)定性好;與電化學(xué)發(fā)光分析技術(shù)(ECLIA)比對,相關(guān)系數(shù)達(dá)0.8898。正常參考值范圍男性為90~350ng/ml,女性為30~260ng/ml。本法建立的FER-TRFIA-POCT是一個(gè)快速高靈敏和可靠的檢測。
[Abstract]:A time-resolved fluorescence immunochromatographic assay (TRFIA-POCTT) was established for the detection of ferritin. FER antibody and rabbit IgG were coated on the nitrocellulose membrane (NC). The sensitivity of FER-TRFIA-POCT was 0.5 ng / ml using fluorescent microspheres labeled monoclonal antibody against FER and rabbit IgG as detection line and quality control line, respectively. The sensitivity of FER-TRFIA-POCT was 0.5 ng / ml, the CV was 3.2g / ml, and the interassay CV was 3.80.2.The average recovery was 100.5 and the thermal stability was good. Compared with ECLIA, the correlation coefficient was 0.8898.The normal reference value range was 90 ~ 350ng / ml for men and 30 ~ 260ng / ml for women. The FER-TRFIA-POCT established by this method was a rapid, sensitive and reliable method.
【作者單位】： 江蘇省原子醫(yī)學(xué)研究所江蘇省分子核醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室衛(wèi)生部核醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室;江陰市人民醫(yī)院檢驗(yàn)科;
【基金】：無錫市衛(wèi)生局科研項(xiàng)目(MS201530)
【分類號】：R446.6
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本文編號：1662275