本文選題：手足口病　切入點(diǎn)：腸道病毒型(EV)　出處：《中國(guó)公共衛(wèi)生》2016年06期 　論文類型：期刊論文

【摘要】：目的分析貴州省貴陽(yáng)市2013—2015年手足口病患兒腸道病毒71型(EV71)VP1和VP4基因特征,為手足口病疫苗的選用和臨床診治提供參考依據(jù)。方法采集2013年8月—2015年4月在貴陽(yáng)市第五人民醫(yī)院就診的120例手足口病輕癥患兒鼻咽拭子和(或)糞便標(biāo)本進(jìn)行EV71病毒VP1和VP4全序列的逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)(RT-PCR)擴(kuò)增和測(cè)序,并與美國(guó)國(guó)立生物技術(shù)信息中心公布的EV71 A、B、C基因型代表株進(jìn)行系統(tǒng)進(jìn)化分析,根據(jù)進(jìn)化分析結(jié)果對(duì)此2個(gè)片段隨機(jī)抽取20例與其他地區(qū)相同基因型進(jìn)行氨基酸位點(diǎn)變異分析。結(jié)果所有樣本病毒株均為C4基因亞型,與2008年北京、上海、安徽以及2011年深圳分離株成簇,20例EV71病毒株在VP1和VP4氨基酸位點(diǎn)變異與以上城市流行毒株相比,其中12例樣本EV7的VP1片段氨基酸序列293位丙氨酸(A)變異為絲氨酸(S),其余位點(diǎn)均只有1例樣本發(fā)生A26T、N31D、N282S突變,而VP4片段只有1個(gè)樣本發(fā)生K52R突變。結(jié)論貴陽(yáng)市2013—2015年手足口病患兒的EV71流行株均屬C基因型的C4亞型,VP1片段常見(jiàn)A293S、A26T、N31D和N282S突變,VP4片段偶有K52R突變。
[Abstract]:Objective to analyze the characteristics of enterovirus 71 (EV71) VP1 and VP4 gene in children with hand-foot-mouth disease (HFMD) from 2013 to 2015 in Guiyang, Guizhou Province. Methods from August 2013 to April 2015, 120 children with hand foot and mouth disease who were treated in Guiyang 5th people's Hospital were collected nasopharyngeal swabs and / or fecal specimens from August 2013 to April 2015 in order to provide reference for the selection, clinical diagnosis and treatment of hand-foot-mouth disease vaccine. EV71 virus VP1 and VP4 sequences were amplified and sequenced by reverse transcription-polymerase chain reaction (RT-PCR). The phylogenetic analysis was carried out with the representative strain of EV71 Agna C genotype published by the National Biotechnology Information Center of the United States. According to the results of evolutionary analysis, 20 samples of these two fragments were randomly selected for amino acid locus variation analysis with the same genotype as other regions. Results all of the sample strains were C4 gene subtypes, which were similar to those of Beijing and Shanghai in 2008. In Anhui and Shenzhen in 2011, 20 strains of EV71 virus were clustered and mutated at the amino acid sites of VP1 and VP4, compared with the epidemic strains in the above cities. Among them, the amino acid sequence of VP1 fragment of EV7 in 12 cases was mutated into serine tassel, and only one of the other samples had a mutation of A26TX N31DN282S. However, K52R mutation was found in only one sample of VP4 fragment in Guiyang from 2013 to 2015. Conclusion K52R mutation is occasionally found in the C genotype C subtype C subtype VP1 fragment of A293SU A26TN 31D and N282S mutation VP4 in children with hand-foot-mouth disease in Guiyang from 2013 to 2015.
【作者單位】： 貴州醫(yī)科大學(xué)檢驗(yàn)學(xué)院;貴陽(yáng)市公共衛(wèi)生救治中心;
【分類號(hào)】：R725.1;R440
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本文編號(hào)：1619286