本文關(guān)鍵詞： 光滑念珠菌 大鼠氣道上皮細(xì)胞 VGX- 腫瘤壞死因子α 白細(xì)胞介素β　出處：《重慶醫(yī)學(xué)》2016年32期 　論文類(lèi)型：期刊論文

【摘要】：目的探討未加免疫抑制劑VGX-1027大鼠氣道上皮細(xì)胞(RTEC)與加入VGX-1027的RTEC分別與光滑念珠菌孵育后炎癥因子IL-1β和TNF-α表達(dá)及意義。方法以體外培養(yǎng)未加VGX-1027孵育的RTEC(未加組)和加入VGX-1027孵育的RTEC(加入組),分別與光滑念珠菌孵育3、6、9h,觀察未加和加入組RTEC形態(tài)變化;Real time PCR檢測(cè)未加和加入組IL-1β和TNF-αmRNA表達(dá);ELISA檢測(cè)未加和加入組IL-1β和TNF-α分泌。未與光滑念珠菌孵育并未加VGX-1027的RTEC為對(duì)照組。結(jié)果隨孵育時(shí)間延長(zhǎng),IL-1β和TNF-α表達(dá)呈進(jìn)行性增高,未加組與加入組相比,未加組IL-1β和TNF-α表達(dá)更高。相同時(shí)間點(diǎn),未加組與加入組比較IL-1β和TNF-α表達(dá)均差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 RTEC可識(shí)別光滑念珠菌啟動(dòng)天然免疫,并激活下游炎癥因子IL-1β和TNF-α的分泌,介導(dǎo)炎癥反應(yīng),但是過(guò)度的炎癥反應(yīng)不能起到保護(hù)作用反而加重?fù)p傷。
[Abstract]:Objective to investigate the expression and significance of inflammatory cytokines IL-1 尾 and TNF- 偽 in airway epithelial cells of VGX-1027 rats without immunosuppressant and RTEC added with VGX-1027, respectively. IL-1 尾 and TNF- 偽 mRNA expression of IL-1 尾 and TNF- 偽 mRNA were detected by Real time PCR after incubation with Candida glabrata for 3 ~ 6 h after incubation with VGX-1027. IL-1 尾 and TNF- 偽 secretion in unadded and added groups were detected by Elisa method, but not smooth and smooth were detected by enzyme-linked immunosorbent immunosorbent assay (Elisa) for detecting the expression of IL-1 尾 and TNF- 偽 mRNA in unadded and added groups. The results showed that the expression of IL-1 尾 and TNF- 偽 increased progressively with the increase of incubation time. The expression of IL-1 尾 and TNF- 偽 in the non-addition group was higher than that in the addition group. At the same time point, the expression of IL-1 尾 and TNF- 偽 in the non-addition group was significantly higher than that in the added group. Conclusion RTEC can recognize the innate immunity of Candida smooth. It also activates the secretion of IL-1 尾 and TNF- 偽 and mediates the inflammatory response, but the excessive inflammatory response does not play a protective role but exacerbates the injury.
【作者單位】： 桂林醫(yī)學(xué)院第二附屬醫(yī)院檢驗(yàn)科;桂林醫(yī)學(xué)院第二附屬醫(yī)院重癥醫(yī)學(xué)科;
【基金】：廣西醫(yī)療衛(wèi)生重點(diǎn)科研課題(重2012007) 廣西自然科學(xué)基金項(xiàng)目(2013GXNSFAA019209)
【分類(lèi)號(hào)】：R459.7

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