本文關(guān)鍵詞： 肺炎克雷伯菌 pNDM-LJ blaNDM-基因 碳青霉烯酶　出處：《實(shí)用醫(yī)學(xué)雜志》2016年05期 　論文類型：期刊論文

【摘要】：目的:通過對(duì)肺炎克雷伯菌質(zhì)粒pNDM-LJ的高通量測(cè)序分析,研究其與肺炎克雷伯菌耐藥的相關(guān)性。方法:利用Illumina Miseq高通量測(cè)序平臺(tái)進(jìn)行測(cè)序,然后用Edena軟件拼接測(cè)序數(shù)據(jù),再利用RAST網(wǎng)上工具將拼接得到的contigs進(jìn)行注釋,并用BLAST網(wǎng)上工具進(jìn)行分析。結(jié)果:pNDM-LJ為54 kb大小的環(huán)狀質(zhì)粒,GC含量約為49%,預(yù)測(cè)編碼52個(gè)功能基因,在不相容群分類中屬于Inc X3群質(zhì)粒。該質(zhì)粒與已知的Inc X質(zhì)粒序列有較高的相似性,blaNDM-1基因所在的基因環(huán)境比較復(fù)雜,推測(cè)其是由多個(gè)轉(zhuǎn)座事件共同構(gòu)建的。在pNDM-LJ中,blaNDM-1基因的5′端和3′端分別與ISAba125和IS26兩種插入序列相連,形成大小約為10.8 kb的轉(zhuǎn)座子樣結(jié)構(gòu)。結(jié)論:pNDM-LJ質(zhì)粒攜帶碳青霉烯類耐藥基因blaNDM-1,可能對(duì)我國(guó)的廣泛傳播blaNDM-1基因發(fā)揮重要作用。
[Abstract]:Objective: through the high-throughput sequencing of Klebsiella pneumoniae plasmid pNDM-LJ analysis, study the correlation with drug resistance of Klebsiella pneumoniae. Methods: Illumina Miseq was sequenced by high-throughput sequencing platform, and then use the Edena software using RAST sequencing data, online tools will be spliced contigs notes, and was analyzed by BLAST. Results: pNDM-LJ online tools for circular plasmid was 54 kb in size, the content of GC is about 49% and the predicted encoding 52 functional genes in incompatible group classification belongs to Inc plasmid. X3 group had high similarity Inc X plasmid sequence of the plasmid with known blaNDM-1 gene, gene environment where the more complex, suggesting that it is constructed by a plurality of transposition events. In pNDM-LJ, the 5 'end of blaNDM-1 gene and the 3' end respectively with ISAba125 and IS26 two kinds of insertion sequence is formed in the size of approximately 10.8 KB transposon Conclusion: the pNDM-LJ plasmid carries the carbapenems resistance gene blaNDM-1, which may play an important role in the wide spread of blaNDM-1 gene in China.

【作者單位】： 廣州醫(yī)科大學(xué)附屬第一醫(yī)院檢驗(yàn)科;廣州醫(yī)科大學(xué)附屬第一醫(yī)院轉(zhuǎn)化醫(yī)學(xué)實(shí)驗(yàn)室;南方醫(yī)科大學(xué)南方醫(yī)院檢驗(yàn)科;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):81201341)
【分類號(hào)】：R446.5
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本文編號(hào)：1538735