本文關(guān)鍵詞： 核酸檢測 血清學檢測 血液篩查　出處：《中國艾滋病性病》2016年03期 　論文類型：期刊論文

【摘要】：目的對溫州地區(qū)無償獻血者的血液標本進行血清學與核酸聯(lián)合檢測,全面評估血清學與核酸檢測(NAT)在降低輸血相關(guān)傳染性疾病中的互補性。方法對2014年10月至2015年6月,溫州市中心血站采集的無償獻血者的血液標本,用酶聯(lián)免疫吸附試驗(ELISA)對血液傳染性指標乙型肝炎病毒表面抗原(HBsAg)、丙型肝炎病毒(HCV)抗體、艾滋病病毒(HIV)抗體、蒼白球螺旋體(TP)抗體進行檢測,丙氨酸轉(zhuǎn)氨酶(ALT)采用速率法,同時采用核酸檢測技術(shù)對乙型肝炎病毒(HBV)DNA、HCV RNA、HIV RNA進行6人份三項目聯(lián)合檢測,并對檢測結(jié)果進行分析。結(jié)果 33 736份血液標本中,ELISA(+)NAT(+)95份,其中HBsAg(+)NAT(+)72份,符合率60.5%;HCV抗體(+)NAT(+)13份,符合率11.2%;HIV抗體(+)NAT(+)10份,符合率30.3%。41份ELISA(-)核酸(+)樣本,且均為HBV DNA陽性。ELISA(+)NAT(-)173份。ELISA雙試劑陽性樣本中,HBV、HCV、HIV S/CO≥5的樣本組的核酸陽性符合率高于S/CO5的樣本組。結(jié)論核酸檢測能有效地降低輸血傳播疾病的發(fā)生,但也存在漏檢的可能,不能完全取代血清學檢測,只有兩者有機結(jié)合才能真正保證血液的安全。
[Abstract]:Objective to detect the serum and nucleic acid of blood samples from unpaid blood donors in Wenzhou area. A comprehensive assessment of the complementarity between serology and nucleic acid testing (Nat) in reducing transfusion-related infectious diseases was conducted from October 2014 to June 2015. Blood samples collected from blood donors from Wenzhou city center blood station were tested by Elisa to detect hepatitis B virus surface antigen (HBsAg). The antibody against hepatitis C virus (HCV), HIV / AIDS (HIV / AIDS) and Treponema pallidum (TPTP) were detected, and alanine aminotransferase (alt) was used as rate method. At the same time, the nucleic acid detection technique was used to detect the RNA of hepatitis B virus (HBV) in hepatitis B virus (HBV) DNA. Results of 33 736 blood samples, 95 were Nat (Nat) and 72 were HBsAg (Nat). The coincidence rate was 60.5%; HCV antibody (Nat 13 copies, coincidence rate 11.2%); HIV antibody (Nat 10, coincidence rate 30.3.41 ELISA-) nucleic acid () samples. All the samples were positive for HBV DNA. The positive coincidence rate of nucleic acid in the HIV / CO 鈮，

本文編號：1443611