本文關(guān)鍵詞：降鈣素原兩種免疫檢測方法的建立　出處：《長春理工大學》2015年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 降鈣素原 膠體金 酶聯(lián)免疫吸附測定 細菌感染

【摘要】：降鈣素原是有活性的降鈣素的初始結(jié)構(gòu)物質(zhì),通常是由甲狀腺C細胞分泌的,在機體中PCT對血液鈣過多的情況有應(yīng)答反應(yīng)。降鈣素原對于病人菌血癥的預(yù)測和評估疾病的嚴重程度上是很重要的依據(jù)。因此醫(yī)學臨床近年對于降鈣素原的研究及體外診斷方法引起學者廣泛關(guān)注。本課題建立了降鈣素原半定量膠體金免疫檢測和ELISA檢測方法。通過試驗對反應(yīng)條件以及實驗用耗材和試劑來進行優(yōu)化,達到最佳檢測效果。對檢測試劑的敏感性、重復(fù)性和特異性進行評估。實驗結(jié)果表明,本研究成功的建立了降鈣素原半定量膠體金免疫檢測方法,并且制作了標準比色卡。檢測范圍為10 ng/ml、2 ng/ml、0.5 ng/ml、0.1 ng/ml,顯色效果和靈敏度較好。性能評估表明研究中建立的試紙條的特異性、重復(fù)性以及熱穩(wěn)定性均表現(xiàn)良好。與市售的國產(chǎn)試劑盒相比顯色梯度明顯,檢測限更低,顯色效果更好。成功建立了ELISA檢測方法,確定了陽性樣品的臨界值OD450nm=0.2395,線性范圍在75 ng/L-1200 ng/L之間,最低檢出限為75 ng/L。性能評估顯示出了本檢測試劑特異性良好,批內(nèi)以及批間變異系數(shù)均小于7%,精密度高。與市售的國產(chǎn)試劑盒相比靈敏度更高,線性更好。
[Abstract]:Procalcitonin is the initial structural substance of active calcitonin, usually secreted by thyroid C cells. In the body, PCT is responsive to the condition of hypercalcemia. Procalcitonin is an important basis for predicting and evaluating the severity of the disease in patients with bacteremia. The research and in vitro diagnosis methods have attracted much attention. In this paper, a semi-quantitative colloidal gold immunoassay and a ELISA detection method for procalcitonin have been established. The reaction conditions and the consumables and reagents used in the experiment have been studied. All right, optimization. The sensitivity, reproducibility and specificity of the reagent were evaluated. The results showed that the semi-quantitative colloidal gold immunoassay for calcitonin was successfully established. The standard colorimetric card was made and the detection range was 10 ng / ml 2 ng / ml 0. 5 ng / ml 0. 1 ng/ml. The performance evaluation showed that the specificity, reproducibility and thermal stability of the test strip were good. Compared with the domestic kit, the color gradient was obvious and the detection limit was lower. The method of ELISA detection was established successfully and the critical value of OD450nm=0.2395 of positive samples was determined. The linear range was between 75 ng/L-1200 ng/L and the lowest detection limit was 75 ng / L. The performance evaluation showed that the specificity of the reagent was good. The coefficient of variation within and between batches is less than 7, and the precision is high. Compared with the domestic kits, the sensitivity is higher and the linearity is better.
【學位授予單位】：長春理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R446.6

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本文編號：1391163