本文關(guān)鍵詞：ISOLA全自動(dòng)平板接種儀對(duì)三種痰消化液液化和接種效果的評(píng)估　出處：《中華醫(yī)院感染學(xué)雜志》2016年20期 　論文類型：期刊論文

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【摘要】：目的評(píng)估ISOLA全自動(dòng)平板接種儀使用Digest-EUR與SPUTASOL、胰蛋白酶痰消化液處理痰標(biāo)本的液化和接種效果,以及不同試劑對(duì)細(xì)菌生長(zhǎng)的影響,為選擇痰消化液提供依據(jù)。方法收集2014年10月-2015年1月醫(yī)院住院患者120份中等粘稠痰標(biāo)本和特粘稠痰標(biāo)本,同時(shí)采用三種試劑對(duì)標(biāo)本進(jìn)行預(yù)消化處理,評(píng)估接種效果,三種試劑分別與肺炎鏈球菌、流感嗜血菌菌液接觸0、15、30、60、90、120min,觀察不同作用時(shí)間對(duì)肺炎鏈球菌和流感嗜血菌生長(zhǎng)的影響,并使用SPSS統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)分析。結(jié)果三種試劑對(duì)兩種粘度痰標(biāo)本消化處理后,進(jìn)行全自動(dòng)標(biāo)本接種均未出現(xiàn)吸頭堵塞,消化預(yù)處理效果差異無(wú)統(tǒng)計(jì)學(xué)意義;在哥倫比亞血平板、中國(guó)藍(lán)平板和巧克力平板上的接種效果差異無(wú)統(tǒng)計(jì)學(xué)意義;Digest-EUR和SPUTASOL兩種試劑與肺炎鏈球菌、流感嗜血菌菌液接觸不同時(shí)間后,均對(duì)生長(zhǎng)無(wú)明顯影響,胰蛋白酶作用15min后,即對(duì)肺炎鏈球菌、流感嗜血菌的生長(zhǎng)產(chǎn)生抑制。結(jié)論三種試劑均可用于ISOLA全自動(dòng)平板接種儀對(duì)痰標(biāo)本的消化預(yù)處理,其中Digest-EUR和SPUTASOL消化液對(duì)肺炎鏈球菌和流感嗜血菌生長(zhǎng)無(wú)明顯影響,胰蛋白酶液化15min后對(duì)肺炎鏈球菌和流感嗜血菌的生長(zhǎng)有明顯的抑制作用。
[Abstract]:Objective to evaluate the effect of liquefaction and inoculation of sputum samples treated with Digest-EUR and SPUTASOL with ISOLA automatic plate inoculation instrument. And the effects of different reagents on the growth of bacteria. Methods from October 2014 to January 2015, 120 cases of moderately viscous sputum and particularly viscous sputum were collected. At the same time, the samples were predigested with three reagents to evaluate the inoculation effect. The three reagents were exposed to Streptococcus pneumoniae and Haemophilus influenzae solution for 120min. The effects of different action time on the growth of Streptococcus pneumoniae and Haemophilus influenzae were observed and analyzed by SPSS software. No head clogging was found in all the fully automatic inoculated specimens, and there was no significant difference in the effect of digestion and pretreatment. There was no significant difference in inoculation effect between Chinese blue plate and chocolate plate in Colombian blood plate. After contact with Streptococcus pneumoniae and Haemophilus influenzae for different time, Digest-EUR and SPUTASOL had no obvious effect on growth. The growth of Streptococcus pneumoniae and Haemophilus influenzae was inhibited. Conclusion the three reagents can be used for digesting and pretreating sputum samples with ISOLA automatic plate inoculation instrument. Digest-EUR and SPUTASOL had no effect on the growth of Streptococcus pneumoniae and Haemophilus influenzae. Trypsin liquefaction for 15 min inhibited the growth of Streptococcus pneumoniae and Haemophilus influenzae.
【作者單位】： 解放軍總醫(yī)院微生物科;梅里埃診斷產(chǎn)品(上海)有限公司;
【基金】：軍隊(duì)“十二五”重大課題分課題(AWS11C001)
【分類號(hào)】：R446.5
【正文快照】： 傳統(tǒng)微生物樣本接種以重復(fù)性的手工操作為主,費(fèi)時(shí)費(fèi)力且難以滿足標(biāo)準(zhǔn)化和可追溯管理的要求。近年來隨著越來越多的全自動(dòng)標(biāo)本前處理系統(tǒng)引入實(shí)驗(yàn)室[1-2],相關(guān)評(píng)估的文獻(xiàn)相繼發(fā)表[3-4]。全自動(dòng)平板接種儀是一種用于劃線接種成品培養(yǎng)基的自動(dòng)化系統(tǒng),可實(shí)現(xiàn)微生物標(biāo)本接種的標(biāo)準(zhǔn)

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